ID   D5_VACCC                Reviewed;         785 AA.
AC   P21010;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Primase D5;
DE            EC=3.6.4.-;
GN   ORFNames=D5R;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Primase which may have roles in initiation of DNA replication
CC       or lagging-strand synthesis. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with A20. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae D5 family. {ECO:0000305}.
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DR   EMBL; M35027; AAA48102.1; -; Genomic_DNA.
DR   PIR; D42515; D42515.
DR   SASBDB; P21010; -.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR004968; DNA_primase/NTPase_C.
DR   InterPro; IPR014015; Helicase_SF3_DNA-vir.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014818; Phage/plasmid_primase_P4_C.
DR   Pfam; PF08706; D5_N; 1.
DR   Pfam; PF03288; Pox_D5; 1.
DR   PROSITE; PS51206; SF3_HELICASE_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..785
FT                   /note="Primase D5"
FT                   /id="PRO_0000099439"
FT   DOMAIN          477..639
FT                   /note="SF3 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT   REGION          342..469
FT                   /note="Primase"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000255"
FT   BINDING         503..510
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
SQ   SEQUENCE   785 AA;  90369 MW;  8F9156509C92164B CRC64;
     MDAAIRGNDV IFVLKTIGVP SACRQNEDPR FVEAFKCDEL KRYIDNNPEC TLFESLRDEE
     AYSIVRIFMD VDLDACLDEI DYLTAIQDFI IEVSNCVARF AFTECGAIHE NVIKSMRSNF
     SLTKSTNRDK TSFHIIFLDT YTTMDTLIAM KRTLLELSRS SENPLTRSID TAVYRRKTTL
     RVVGTRKNPN CDTIHVMQPP HDNIEDYLFT YVDMNNNSYY FSLQRRLEDL VPDKLWEPGF
     ISFEDAIKRV SKIFINSIIN FNDLDENNFT TVPLVIDYVT PCALCKKRSH KHPHQLSLEN
     GAIRIYKTGN PHSCKVKIVP LDGNKLFNIA QRILDTNSVL LTERGDYIVW INNSWKFNSE
     EPLITKLILS IRHQLPKEYS SELLCPRKRK TVEANIRDML VDSVETDTYP DKLPFKNGVL
     DLVDGMFYSG DDAKKYTCTV STGFKFDDTK FVEDSPEMEE LMNIINDIQP LTDENKKNRE
     LYEKTLSSCL CGATKGCLTF FFGETATGKS TTKRLLKSAI GDLFVETGQT ILTDVLDKGP
     NPFIANMHLK RSVFCSELPD FACSGSKKIR SDNIKKLTEP CVIGRPCFSN KINNRNHATI
     IIDTNYKPVF DRIDNALMRR IAVVRFRTHF SQPSGREAAE NNDAYDKVKL LDEGLDGKIQ
     NNRYRFAFLY LLVKWYKKYH VPIMKLYPTP EEIPDFAFYL KIGTLLVSSS VKHIPLMTDL
     SKKGYILYDN VVTLPLTTFQ QKISKYFNSR LFGHDIESFI NRHKKFANVS DEYLQYIFIE
     DISSP