ID   D5_VACCW                Reviewed;         785 AA.
AC   P04305; Q76ZS2;
DT   20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-1987, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Primase D5;
DE            EC=3.6.4.-;
GN   OrderedLocusNames=VACWR110; ORFNames=D5R;
OS   Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS   WR)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10254;
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3739227; DOI=10.1016/0042-6822(86)90011-5;
RA   Niles E.G., Condit R.C., Caro P., Davidson K., Matusick L., Seto J.;
RT   "Nucleotide sequence and genetic map of the 16-kb vaccinia virus HindIII D
RT   fragment.";
RL   Virology 153:96-112(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3033268; DOI=10.1128/jvi.61.5.1398-1406.1987;
RA   Roseman N.A., Hruby D.E.;
RT   "Nucleotide sequence and transcript organization of a region of the
RT   vaccinia virus genome which encodes a constitutively expressed gene
RT   required for DNA replication.";
RL   J. Virol. 61:1398-1406(1987).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA   Wohlhueter R.;
RT   "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT   redundancy and an error rate of 0.16/10kb.";
RL   Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   INTERACTION WITH A20.
RX   PubMed=12490386; DOI=10.1006/viro.2002.1721;
RA   Ishii K., Moss B.;
RT   "Mapping interaction sites of the A20R protein component of the vaccinia
RT   virus DNA replication complex.";
RL   Virology 303:232-239(2002).
RN   [5]
RP   FUNCTION, AND MUTAGENESIS OF ASP-170 AND GLY-503.
RX   PubMed=18000036; DOI=10.1073/pnas.0709276104;
RA   De Silva F.S., Lewis W., Berglund P., Koonin E.V., Moss B.;
RT   "Poxvirus DNA primase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:18724-18729(2007).
CC   -!- FUNCTION: Primase which may have roles in initiation of DNA replication
CC       or lagging-strand synthesis. {ECO:0000269|PubMed:18000036}.
CC   -!- SUBUNIT: Interacts with A20. {ECO:0000269|PubMed:12490386}.
CC   -!- SIMILARITY: Belongs to the poxviridae D5 family. {ECO:0000305}.
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DR   EMBL; M15058; AAA48259.1; -; Genomic_DNA.
DR   EMBL; M16021; AAA69629.1; -; Genomic_DNA.
DR   EMBL; AY243312; AAO89389.1; -; Genomic_DNA.
DR   PIR; B93025; QQVZ8.
DR   RefSeq; YP_232992.1; NC_006998.1.
DR   DIP; DIP-2181N; -.
DR   IntAct; P04305; 1.
DR   MINT; P04305; -.
DR   PRIDE; P04305; -.
DR   GeneID; 3707566; -.
DR   KEGG; vg:3707566; -.
DR   Proteomes; UP000000344; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003896; F:DNA primase activity; IDA:UniProtKB.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0017111; F:nucleoside-triphosphatase activity; IDA:UniProtKB.
DR   GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IDA:UniProtKB.
DR   GO; GO:0039693; P:viral DNA genome replication; IDA:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR004968; DNA_primase/NTPase_C.
DR   InterPro; IPR014015; Helicase_SF3_DNA-vir.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR014818; Phage/plasmid_primase_P4_C.
DR   Pfam; PF08706; D5_N; 1.
DR   Pfam; PF03288; Pox_D5; 1.
DR   PROSITE; PS51206; SF3_HELICASE_1; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..785
FT                   /note="Primase D5"
FT                   /id="PRO_0000099440"
FT   DOMAIN          477..639
FT                   /note="SF3 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT   REGION          342..469
FT                   /note="Primase"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000255"
FT   BINDING         503..510
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT   MUTAGEN         170
FT                   /note="D->A: Loss of primase activity."
FT                   /evidence="ECO:0000269|PubMed:18000036"
FT   MUTAGEN         503
FT                   /note="G->A: Loss of NTPase activity, no effect on primase
FT                   activity."
FT                   /evidence="ECO:0000269|PubMed:18000036"
SQ   SEQUENCE   785 AA;  90357 MW;  317984B1B7FE21D7 CRC64;
     MDAAIRGNDV IFVLKTIGVP SACRQNEDPR FVEAFKCDEL ERYIENNPEC TLFESLRDEE
     AYSIVRIFMD VDLDACLDEI DYLTAIQDFI IEVSNCVARF AFTECGAIHE NVIKSMRSNF
     SLTKSTNRDK TSFHIIFLDT YTTMDTLIAM KRTLLELSRS SENPLTRSID TAVYRRKTTL
     RVVGTRKNPN CDTIHVMQPP HDNIEDYLFT YVDMNNNSYY FSLQQRLEDL VPDKLWEPGF
     ISFEDAIKRV SKIFINSIIN FNDLDENNFT TVPLVIDYVT PCALCKKRSH KHPHQLSLEN
     GAIRIYKTGN PHSCKVKIVP LDGNKLFNIA QRILDTNSVL LTERGDHIVW INNSWKFNSE
     EPLITKLILS IRHQLPKEYS SELLCPRKRK TVEANIRDML VDSVETDTYP DKLPFKNGVL
     DLVDGMFYSG DDAKKYTCTV STGFKFDDTK FVEDSPEMEE LMNIINDIQP LTDENKKNRE
     LYEKTLSSCL CGATKGCLTF FFGETATGKS TTKRLLKSAI GDLFVETGQT ILTDVLDKGP
     NPFIANMHLK RSVFCSELPD FACSGSKKIR SDNIKKLTEP CVIGRPCFSN KINNRNHATI
     IIDTNYKPVF DRIDNALMRR IAVVRFRTHF SQPSGREAAE NNDAYDKVKL LDEGLDGKIQ
     NNRYRFAFLY LLVKWYRKYH VPIMKLYPTP EEIPDFAFYL KIGTLLVSSS VKHIPLMTDL
     SKKGYILYDN VVTLPLTTFQ QKISKYFNSR LFGHDIESFI NRHKKFANVS DEYLQYIFIE
     DISSP