ID   D5_VARV                 Reviewed;         785 AA.
AC   P0DST8; P33069;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   03-AUG-2022, entry version 11.
DE   RecName: Full=Primase D5;
DE            EC=3.6.4.-;
GN   ORFNames=D5R, F5R;
OS   Variola virus.
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10255;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Bangladesh-1975;
RX   PubMed=8264798; DOI=10.1038/366748a0;
RA   Massung R.F., Esposito J.J., Liu L.I., Qi J., Utterback T.R., Knight J.C.,
RA   Aubin L., Yuran T.E., Parsons J.M., Loparev V.N., Selivanov N.A.,
RA   Cavallaro K.F., Kerlavage A.R., Mahy B.W.J., Venter J.C.;
RT   "Potential virulence determinants in terminal regions of variola smallpox
RT   virus genome.";
RL   Nature 366:748-751(1993).
CC   -!- FUNCTION: Primase which may have roles in initiation of DNA replication
CC       or lagging-strand synthesis. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with A20. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the poxviridae D5 family. {ECO:0000305}.
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DR   EMBL; L22579; AAA60843.1; -; Genomic_DNA.
DR   PIR; B36847; B36847.
DR   PIR; T28533; T28533.
DR   RefSeq; NP_042139.1; NC_001611.1.
DR   GeneID; 1486421; -.
DR   KEGG; vg:1486421; -.
DR   Proteomes; UP000119805; Genome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   InterPro; IPR004968; DNA_primase/NTPase_C.
DR   InterPro; IPR014015; Helicase_SF3_DNA-vir.
DR   InterPro; IPR014818; Phage/plasmid_primase_P4_C.
DR   Pfam; PF08706; D5_N; 1.
DR   Pfam; PF03288; Pox_D5; 1.
DR   PROSITE; PS51206; SF3_HELICASE_1; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Helicase; Hydrolase; Nucleotide-binding.
FT   CHAIN           1..785
FT                   /note="Primase D5"
FT                   /id="PRO_0000448178"
FT   DOMAIN          477..639
FT                   /note="SF3 helicase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
FT   REGION          342..469
FT                   /note="Primase"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000255"
FT   BINDING         503..510
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00551"
SQ   SEQUENCE   785 AA;  90471 MW;  CBB873AD8C1F367B CRC64;
     MDAVIRGNDV IFVLKTIGVP SVCRQNEDPR FVEAFKCDEL ERYIKNNPEC TLFESLRDEE
     AYSIVRIFMD VDLDACLDEI DYLTAIQDFI IEVSNCVARF AFTECGAIHE NVIKSMRSNF
     SLTKSTNRDK TSFHIIFLDT YTTMDTLIAM KRTLLELSRS SENPLTRSID TAVYRKKTTL
     RVVGTRKNPN CDTIHVMQPP HDNIEDYLFT YVDMNNNSYY FSLQRRLEDL VPDKLWEPGF
     ISFEDAIKRV SKIFINSIIN FNDLDENNFT TVPLVIDYVT PCALCKKRSH KHPHQLSLEN
     DAIRIYKTGN PHSCKVKIVP LDGNKLFNIA QRILDTNSVL LTERGDHIVW INNSWKFNSE
     EPLITKLILS IRHQLPKEYS SELLCPRKRK TVEANIRDML VDSVETDTYP DKLPFKNGVL
     DLVDGMFYSG DDAKKYTCTV STGFKFDDTK FVEDSPEMEE LINIINDIQP LTDENKKNRE
     LYEKTLSSCL CGATKGCLTF FFGETATGKS TTKRLLKSAI SDLFVETGQT ILTDVLDKGP
     NPFIANMHLK RSVFCSELPD FACSGTKKIR SDNIKKLTEP CVIGRPCFSN KINNRNHATI
     IIDTNYKPVF DRIDNALMRR IAVVRFRTHF SQPSGREAAE NNDAYDKVKL LDEGLDGKIQ
     NNRYRFAFLY LLVKWYRKYH VPIMKLYPTP EEIPDFAFYL KIGTLLVSSS VKHIPLMTDL
     SKKGYILHDN VVTLPLTTFQ QKISKYFNSR LFGHDIESFI NRHKKFANVS DEYLQYIFIE
     DISSP