DAA10_HUMAN
ID DAA10_HUMAN Reviewed; 357 AA.
AC Q96MX6; Q96CR6;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 171.
DE RecName: Full=Dynein axonemal assembly factor 10 {ECO:0000305};
DE AltName: Full=WD repeat-containing protein 92;
DE AltName: Full=WD repeat-containing protein Monad {ECO:0000303|PubMed:16487927};
GN Name=DNAAF10 {ECO:0000312|HGNC:HGNC:25176}; Synonyms=WDR92;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=B-cell, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=16487927; DOI=10.1016/j.bbrc.2006.02.009;
RA Saeki M., Irie Y., Ni L., Yoshida M., Itsuki Y., Kamisaki Y.;
RT "Monad, a WD40 repeat protein, promotes apoptosis induced by TNF-alpha.";
RL Biochem. Biophys. Res. Commun. 342:568-572(2006).
RN [5]
RP INTERACTION WITH PIH1D1.
RX PubMed=21078300; DOI=10.1016/j.bbrc.2010.11.031;
RA Inoue M., Saeki M., Egusa H., Niwa H., Kamisaki Y.;
RT "PIH1D1, a subunit of R2TP complex, inhibits doxorubicin-induced
RT apoptosis.";
RL Biochem. Biophys. Res. Commun. 403:340-344(2010).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP IDENTIFICATION IN THE PAQOSOME COMPLEX, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=31738558; DOI=10.1021/acs.jproteome.9b00599;
RA Cloutier P., Poitras C., Faubert D., Bouchard A., Blanchette M.,
RA Gauthier M.S., Coulombe B.;
RT "Upstream ORF-Encoded ASDURF Is a Novel Prefoldin-like Subunit of the
RT PAQosome.";
RL J. Proteome Res. 19:18-27(2020).
RN [8]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS).
RX PubMed=21468892; DOI=10.1007/s13238-011-1018-1;
RA Xu C., Min J.;
RT "Structure and function of WD40 domain proteins.";
RL Protein Cell 2:202-214(2011).
CC -!- FUNCTION: Key assembly factor specifically required for the stability
CC of axonemal dynein heavy chains in cytoplasm.
CC {ECO:0000250|UniProtKB:A8J3F6}.
CC -!- SUBUNIT: Component of the PAQosome complex which is responsible for the
CC biogenesis of several protein complexes and which consists of R2TP
CC complex members RUVBL1, RUVBL2, RPAP3 and PIH1D1, URI complex members
CC PFDN2, PFDN6, PDRG1, UXT and URI1 as well as ASDURF, POLR2E and
CC DNAAF10/WDR92 (PubMed:31738558). Interacts with PIH1D1; the interaction
CC associates DNAAF10 with the R2TP complex (PubMed:21078300). Interacts
CC with several dynein axonemal assembly factors (By similarity).
CC {ECO:0000250|UniProtKB:A8J3F6, ECO:0000269|PubMed:21078300,
CC ECO:0000269|PubMed:31738558}.
CC -!- INTERACTION:
CC Q96MX6; Q9H6T3: RPAP3; NbExp=5; IntAct=EBI-2434101, EBI-356928;
CC -!- SUBCELLULAR LOCATION: Dynein axonemal particle
CC {ECO:0000250|UniProtKB:A8J3F6}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96MX6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96MX6-2; Sequence=VSP_045123;
CC -!- TISSUE SPECIFICITY: Widely expressed with the highest expression in
CC testis. {ECO:0000269|PubMed:16487927}.
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DR EMBL; AK056303; BAB71143.1; -; mRNA.
DR EMBL; AC017083; AAY14713.1; -; Genomic_DNA.
DR EMBL; BC014022; AAH14022.2; -; mRNA.
DR EMBL; BC066657; AAH66657.1; -; mRNA.
DR CCDS; CCDS1884.1; -. [Q96MX6-1]
DR CCDS; CCDS58712.1; -. [Q96MX6-2]
DR RefSeq; NP_001243405.1; NM_001256476.1. [Q96MX6-2]
DR RefSeq; NP_612467.1; NM_138458.3. [Q96MX6-1]
DR PDB; 3I2N; X-ray; 1.95 A; A=1-357.
DR PDBsum; 3I2N; -.
DR AlphaFoldDB; Q96MX6; -.
DR SMR; Q96MX6; -.
DR BioGRID; 125479; 122.
DR ComplexPortal; CPX-6145; PAQosome co-chaperone complex.
DR IntAct; Q96MX6; 35.
DR MINT; Q96MX6; -.
DR STRING; 9606.ENSP00000295121; -.
DR iPTMnet; Q96MX6; -.
DR PhosphoSitePlus; Q96MX6; -.
DR SwissPalm; Q96MX6; -.
DR BioMuta; WDR92; -.
DR DMDM; 74760987; -.
DR EPD; Q96MX6; -.
DR jPOST; Q96MX6; -.
DR MassIVE; Q96MX6; -.
DR MaxQB; Q96MX6; -.
DR PaxDb; Q96MX6; -.
DR PeptideAtlas; Q96MX6; -.
DR PRIDE; Q96MX6; -.
DR ProteomicsDB; 76211; -.
DR ProteomicsDB; 77429; -. [Q96MX6-1]
DR Antibodypedia; 48145; 112 antibodies from 23 providers.
DR DNASU; 116143; -.
DR Ensembl; ENST00000295121.11; ENSP00000295121.6; ENSG00000243667.7. [Q96MX6-1]
DR Ensembl; ENST00000409164.1; ENSP00000386746.1; ENSG00000243667.7. [Q96MX6-2]
DR GeneID; 116143; -.
DR KEGG; hsa:116143; -.
DR MANE-Select; ENST00000295121.11; ENSP00000295121.6; NM_138458.4; NP_612467.1.
DR UCSC; uc002see.3; human. [Q96MX6-1]
DR CTD; 116143; -.
DR DisGeNET; 116143; -.
DR GeneCards; DNAAF10; -.
DR HGNC; HGNC:25176; DNAAF10.
DR HPA; ENSG00000243667; Low tissue specificity.
DR MIM; 610729; gene.
DR neXtProt; NX_Q96MX6; -.
DR OpenTargets; ENSG00000243667; -.
DR VEuPathDB; HostDB:ENSG00000243667; -.
DR eggNOG; KOG0034; Eukaryota.
DR GeneTree; ENSGT00950000183091; -.
DR HOGENOM; CLU_062543_0_0_1; -.
DR InParanoid; Q96MX6; -.
DR OMA; RWEATMK; -.
DR PhylomeDB; Q96MX6; -.
DR TreeFam; TF351064; -.
DR PathwayCommons; Q96MX6; -.
DR SignaLink; Q96MX6; -.
DR BioGRID-ORCS; 116143; 558 hits in 1096 CRISPR screens.
DR ChiTaRS; WDR92; human.
DR EvolutionaryTrace; Q96MX6; -.
DR GenomeRNAi; 116143; -.
DR Pharos; Q96MX6; Tbio.
DR PRO; PR:Q96MX6; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q96MX6; protein.
DR Bgee; ENSG00000243667; Expressed in right uterine tube and 105 other tissues.
DR ExpressionAtlas; Q96MX6; baseline and differential.
DR Genevisible; Q96MX6; HS.
DR GO; GO:0120293; C:dynein axonemal particle; ISS:UniProtKB.
DR GO; GO:1990062; C:RPAP3/R2TP/prefoldin-like complex; IPI:ComplexPortal.
DR GO; GO:0043130; F:ubiquitin binding; IBA:GO_Central.
DR GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR GO; GO:0070286; P:axonemal dynein complex assembly; ISS:UniProtKB.
DR GO; GO:0050821; P:protein stabilization; IC:ComplexPortal.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 2.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Apoptosis; Cytoplasm;
KW Reference proteome; Repeat; WD repeat.
FT CHAIN 1..357
FT /note="Dynein axonemal assembly factor 10"
FT /id="PRO_0000301671"
FT REPEAT 63..105
FT /note="WD 1"
FT REPEAT 115..154
FT /note="WD 2"
FT REPEAT 162..205
FT /note="WD 3"
FT REPEAT 207..249
FT /note="WD 4"
FT REPEAT 257..297
FT /note="WD 5"
FT REPEAT 319..357
FT /note="WD 6"
FT VAR_SEQ 289..357
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_045123"
FT VARIANT 241
FT /note="M -> V (in dbSNP:rs13009282)"
FT /id="VAR_053438"
FT STRAND 9..16
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 18..26
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 30..38
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 44..51
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 53..66
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 68..72
FT /evidence="ECO:0007829|PDB:3I2N"
FT TURN 78..80
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 83..87
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 92..95
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 104..107
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 114..121
FT /evidence="ECO:0007829|PDB:3I2N"
FT HELIX 122..124
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 131..136
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 141..144
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 153..156
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 167..173
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 182..187
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 190..196
FT /evidence="ECO:0007829|PDB:3I2N"
FT TURN 197..200
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 201..207
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 212..218
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 220..223
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 226..232
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 235..240
FT /evidence="ECO:0007829|PDB:3I2N"
FT TURN 246..248
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 252..256
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 262..268
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 271..279
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 282..290
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 310..318
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 324..329
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 331..333
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 336..341
FT /evidence="ECO:0007829|PDB:3I2N"
FT STRAND 344..351
FT /evidence="ECO:0007829|PDB:3I2N"
SQ SEQUENCE 357 AA; 39740 MW; CD48E8A09D8A8F95 CRC64;
MSAFEKPQII AHIQKGFNYT VFDCKWVPCS AKFVTMGNFA RGTGVIQLYE IQHGDLKLLR
EIEKAKPIKC GTFGATSLQQ RYLATGDFGG NLHIWNLEAP EMPVYSVKGH KEIINAIDGI
GGLGIGEGAP EIVTGSRDGT VKVWDPRQKD DPVANMEPVQ GENKRDCWTV AFGNAYNQEE
RVVCAGYDNG DIKLFDLRNM ALRWETNIKN GVCSLEFDRK DISMNKLVAT SLEGKFHVFD
MRTQHPTKGF ASVSEKAHKS TVWQVRHLPQ NRELFLTAGG AGGLHLWKYE YPIQRSKKDS
EGIEMGVAGS VSLLQNVTLS TQPISSLDWS PDKRGLCVCS SFDQTVRVLI VTKLNKI
