ID   DAAF1_BOVIN             Reviewed;         643 AA.
AC   Q3SYS4;
DT   10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-FEB-2009, sequence version 2.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Dynein axonemal assembly factor 1;
DE   AltName: Full=Leucine-rich repeat-containing protein 50;
GN   Name=DNAAF1; Synonyms=LRRC50;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Testis;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Cilium-specific protein required for the stability of the
CC       ciliary architecture. Plays a role in cytoplasmic preassembly of dynein
CC       arms (By similarity). Involved in regulation of microtubule-based cilia
CC       and actin-based brush border microvilli (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DNAAF1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI03421.1; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; BC103420; AAI03421.1; ALT_SEQ; mRNA.
DR   RefSeq; NP_001030422.2; NM_001035345.2.
DR   AlphaFoldDB; Q3SYS4; -.
DR   SMR; Q3SYS4; -.
DR   STRING; 9913.ENSBTAP00000024994; -.
DR   PaxDb; Q3SYS4; -.
DR   PRIDE; Q3SYS4; -.
DR   GeneID; 523187; -.
DR   KEGG; bta:523187; -.
DR   CTD; 123872; -.
DR   eggNOG; ENOG502QQFE; Eukaryota.
DR   InParanoid; Q3SYS4; -.
DR   OrthoDB; 891307at2759; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR   GO; GO:0070840; F:dynein complex binding; ISS:UniProtKB.
DR   GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR   GO; GO:0036159; P:inner dynein arm assembly; IEA:InterPro.
DR   GO; GO:0036158; P:outer dynein arm assembly; IEA:InterPro.
DR   Gene3D; 3.80.10.10; -; 2.
DR   InterPro; IPR027734; DNAAF1.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   PANTHER; PTHR45973:SF19; PTHR45973:SF19; 1.
DR   PROSITE; PS51450; LRR; 6.
PE   2: Evidence at transcript level;
KW   Cell projection; Cilium; Leucine-rich repeat; Phosphoprotein;
KW   Reference proteome; Repeat.
FT   CHAIN           1..643
FT                   /note="Dynein axonemal assembly factor 1"
FT                   /id="PRO_0000363929"
FT   REPEAT          107..129
FT                   /note="LRR 1"
FT   REPEAT          130..151
FT                   /note="LRR 2"
FT   REPEAT          152..173
FT                   /note="LRR 3"
FT   REPEAT          174..195
FT                   /note="LRR 4"
FT   REPEAT          196..217
FT                   /note="LRR 5"
FT   REPEAT          221..242
FT                   /note="LRR 6"
FT   DOMAIN          256..294
FT                   /note="LRRCT"
FT   REGION          1..85
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          332..405
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          537..643
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        32..47
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        50..71
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        364..399
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        544..558
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        615..643
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         477
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9D2H9"
FT   MOD_RES         500
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9D2H9"
SQ   SEQUENCE   643 AA;  71786 MW;  4A321667AD5B014A CRC64;
     MHPEASEPVV DGVAEQECAQ EPGVEESAGD HGNAGEGGRK EEMNDPKETC VGPLNTSCLS
     EQKQSGDSWS DGRLAHQRAD KEDRGARMTK KFLQKLCKQH KLYITPALND TLYLHYKGFD
     RIENLEEYTG LRCLWLECNG IQKIENLEAQ TELRCLFLQV NLLHKIENLE PLQKLDALNI
     SNNYIKTIEN LSCLPVLNTL QMAHNHLETV EDIQHLRECA RLCVLDLSHN KLSDPEILRV
     LESMPDLRVL NLMGNPVIKN IPNYRRILTV RLKHLTYLDD RPVFPKDRAC AEAWARGGYA
     AEKEERQQRE MRERKKITDS IEALAALRRQ AEERKWQRAS QEQGEEPTPA GAGDVDAEVE
     GEEEPQEKDT RQKIEQFVKE SFEAKDELFP EKPEEPSLLE ELPLDVGEPK GVLPSEVLPA
     SPRAAWAELA PQTVATESAS GTEPDGAENL EATRLETKEQ LFIDDLPNLE DVNAGESLGD
     RDREQCFPKI TAISSLSDAS EPDMDDISLL ALENTSSDTL SSIFAVPKDT SWRVETETPF
     TDILKVAPER DPETQRKASE VPRPLIQELG DDEEPSGPPP LPPVCEGEAV PAPCTEDSDG
     DLLPAPAPGN GPEKDEVQSE VEPEERLGSE AGENREDIEF GLD