DAAF1_CHLRE
ID DAAF1_CHLRE Reviewed; 432 AA.
AC Q09JZ4; A8HQD4;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Leucine-rich repeat-containing protein ODA7;
DE AltName: Full=Dynein assembly factor 1, axonemal homolog;
DE AltName: Full=Leucine-rich repeat-containing protein 50 homolog;
DE AltName: Full=Outer row dynein-assembly protein 7;
GN Name=ODA7; ORFNames=CHLREDRAFT_116664;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND INTERACTION WITH DYNEINS.
RX PubMed=17194703; DOI=10.1074/jbc.m607509200;
RA Freshour J., Yokoyama R., Mitchell D.R.;
RT "Chlamydomonas flagellar outer row dynein assembly protein ODA7 interacts
RT with both outer row and I1 inner row dyneins.";
RL J. Biol. Chem. 282:5404-5412(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503, and cw92;
RX PubMed=17932292; DOI=10.1126/science.1143609;
RA Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J.,
RA Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L.,
RA Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H.,
RA Kapitonov V.V., Ren Q., Ferris P., Lindquist E., Shapiro H., Lucas S.M.,
RA Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L.,
RA Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H.,
RA Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M.,
RA Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A.,
RA Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L.,
RA Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C.,
RA Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J.,
RA Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L.,
RA Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L.,
RA Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C.,
RA Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J.,
RA Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P.,
RA Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R.,
RA Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P.,
RA Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y.,
RA Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L.,
RA Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.;
RT "The Chlamydomonas genome reveals the evolution of key animal and plant
RT functions.";
RL Science 318:245-250(2007).
CC -!- FUNCTION: Cilium-specific protein required for cilia structures.
CC Axonemal dynein-associated protein that participates in a structural
CC link between inner and outer row dyneins.
CC {ECO:0000269|PubMed:17194703}.
CC -!- SUBUNIT: Interacts with both outer row and I1 inner row dyneins.
CC {ECO:0000269|PubMed:17194703}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, cilium axoneme
CC {ECO:0000269|PubMed:17194703}.
CC -!- DISRUPTION PHENOTYPE: Absence of outer row dynein and a reduced
CC flagellar beat. Axonemal outer row dynein assembly is prevented by
CC blocking association of heavy chains and intermediate chains in the
CC cytoplasm. {ECO:0000269|PubMed:17194703}.
CC -!- SIMILARITY: Belongs to the DNAAF1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EDP09771.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; DQ886489; ABI63572.1; -; mRNA.
DR EMBL; DS496108; EDP09771.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_001690033.1; XM_001689981.1.
DR AlphaFoldDB; Q09JZ4; -.
DR SMR; Q09JZ4; -.
DR STRING; 3055.EDP09771; -.
DR PaxDb; Q09JZ4; -.
DR EnsemblPlants; PNW88430; PNW88430; CHLRE_01g029150v5.
DR GeneID; 5715340; -.
DR Gramene; PNW88430; PNW88430; CHLRE_01g029150v5.
DR KEGG; cre:CHLRE_01g029150v5; -.
DR eggNOG; ENOG502QQFE; Eukaryota.
DR HOGENOM; CLU_040741_1_0_1; -.
DR InParanoid; Q09JZ4; -.
DR OrthoDB; 891307at2759; -.
DR GO; GO:0005930; C:axoneme; IEA:InterPro.
DR GO; GO:0044297; C:cell body; IDA:BHF-UCL.
DR GO; GO:0005737; C:cytoplasm; IDA:BHF-UCL.
DR GO; GO:0070840; F:dynein complex binding; IEA:InterPro.
DR GO; GO:0070286; P:axonemal dynein complex assembly; IMP:BHF-UCL.
DR GO; GO:0003341; P:cilium movement; IMP:BHF-UCL.
DR GO; GO:0036159; P:inner dynein arm assembly; IEA:InterPro.
DR GO; GO:0036158; P:outer dynein arm assembly; IEA:InterPro.
DR GO; GO:2000155; P:positive regulation of cilium-dependent cell motility; IMP:BHF-UCL.
DR GO; GO:0033365; P:protein localization to organelle; IMP:BHF-UCL.
DR GO; GO:0003356; P:regulation of cilium beat frequency; IMP:BHF-UCL.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR027734; DNAAF1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR PANTHER; PTHR45973:SF19; PTHR45973:SF19; 1.
DR PROSITE; PS51450; LRR; 6.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Cytoskeleton;
KW Leucine-rich repeat; Repeat.
FT CHAIN 1..432
FT /note="Leucine-rich repeat-containing protein ODA7"
FT /id="PRO_0000363967"
FT REPEAT 47..68
FT /note="LRR 1"
FT REPEAT 69..90
FT /note="LRR 2"
FT REPEAT 91..112
FT /note="LRR 3"
FT REPEAT 113..134
FT /note="LRR 4"
FT REPEAT 138..159
FT /note="LRR 5"
FT DOMAIN 173..211
FT /note="LRRCT"
FT REGION 297..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 368..432
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 212..243
FT /evidence="ECO:0000255"
SQ SEQUENCE 432 AA; 47070 MW; A0999CB069D11542 CRC64;
MCACMTKEAL LEVCKQNGLY RTASLNDKLY CNFKGFSQIA CLEDYVNLKA LFLEGNVLET
LEGLPPLADL KCLYVQQNCI WKISGLEAVP GLDTLNISNN QLTKLEGLAC CPALRTLIAT
HNHLVTLDSV AHLAECKALQ TLDLQNNELE DPGIVDILKQ IPDLRCLYLK GNPVVSNIKN
YRKVLVTSIP SLTYLDDRPV FDNERKIAQA WLEGGLEGER AMRNQLKEEE EERSRKNHEF
MMQMRAAGWR ERRKRMGLPD GDTDPALDDM SDGEYEFDEE PEELVEARQR LAAYTARPGE
EEPAELASAR QGLARDGKPI QEGAWGSGAA AESDSAIYLQ SVKAAQAELD VVRQQQPRQL
PTAQVLIEEL DEPCGGKAPA ADEGSTPPAL SPMTSPSGSE GQGGEGVAAA KKGAASGAAE
GISAAVDIND LD
