DAAF1_DROME
ID DAAF1_DROME Reviewed; 1483 AA.
AC Q8INT5; Q6YNS3;
DT 10-FEB-2009, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Dynein axonemal assembly factor 1 homolog;
DE AltName: Full=Defective transmitter-recycling protein;
DE AltName: Full=Leucine-rich repeat-containing protein 50 homolog;
GN Name=dtr; ORFNames=CG31623;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Oregon-R;
RA Lee H.S., Burg M.G., Sarthy P.V., Leung H.-T., Koliantz G., Pak W.L.;
RT "Molecular characterization of the Drosophila dtr gene encoding a novel
RT protein involved in neurotransmitter distribution and recycling.";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Cilium-specific protein required for cilia structures.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNAAF1 family. {ECO:0000305}.
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DR EMBL; AY049773; AAL12209.1; -; mRNA.
DR EMBL; AE014134; AAN11119.2; -; Genomic_DNA.
DR RefSeq; NP_724335.2; NM_165375.3.
DR AlphaFoldDB; Q8INT5; -.
DR SMR; Q8INT5; -.
DR IntAct; Q8INT5; 7.
DR STRING; 7227.FBpp0081044; -.
DR PaxDb; Q8INT5; -.
DR PRIDE; Q8INT5; -.
DR EnsemblMetazoa; FBtr0081516; FBpp0081044; FBgn0023090.
DR GeneID; 318856; -.
DR KEGG; dme:Dmel_CG31623; -.
DR UCSC; CG31623-RA; d. melanogaster.
DR CTD; 318856; -.
DR FlyBase; FBgn0023090; dtr.
DR VEuPathDB; VectorBase:FBgn0023090; -.
DR eggNOG; ENOG502QQFE; Eukaryota.
DR GeneTree; ENSGT00940000158494; -.
DR HOGENOM; CLU_001523_0_0_1; -.
DR InParanoid; Q8INT5; -.
DR OMA; WKREGYE; -.
DR OrthoDB; 162620at2759; -.
DR PhylomeDB; Q8INT5; -.
DR BioGRID-ORCS; 318856; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Trxr-1; fly.
DR GenomeRNAi; 318856; -.
DR PRO; PR:Q8INT5; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0023090; Expressed in testis and 7 other tissues.
DR Genevisible; Q8INT5; DM.
DR GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR GO; GO:0045202; C:synapse; IEA:GOC.
DR GO; GO:0070840; F:dynein complex binding; ISS:UniProtKB.
DR GO; GO:0035082; P:axoneme assembly; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IMP:FlyBase.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0036159; P:inner dynein arm assembly; IEA:InterPro.
DR GO; GO:0036158; P:outer dynein arm assembly; IEA:InterPro.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR027734; DNAAF1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR PANTHER; PTHR45973:SF9; PTHR45973:SF9; 1.
DR PROSITE; PS51450; LRR; 6.
PE 2: Evidence at transcript level;
KW Cell projection; Cilium; Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..1483
FT /note="Dynein axonemal assembly factor 1 homolog"
FT /id="PRO_0000363937"
FT REPEAT 34..56
FT /note="LRR 1"
FT REPEAT 57..78
FT /note="LRR 2"
FT REPEAT 79..100
FT /note="LRR 3"
FT REPEAT 101..122
FT /note="LRR 4"
FT REPEAT 125..146
FT /note="LRR 5"
FT REPEAT 150..171
FT /note="LRR 6"
FT DOMAIN 185..223
FT /note="LRRCT"
FT REGION 249..282
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 300..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 945..986
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1167..1213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 252..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..327
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1167..1185
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 1110
FT /note="D -> E (in Ref. 1; AAL12209)"
FT /evidence="ECO:0000305"
FT CONFLICT 1269
FT /note="R -> C (in Ref. 1; AAL12209)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1483 AA; 168194 MW; D4FF9825C472E604 CRC64;
MSNSNRKEIT GLNRMTPKGL KELCKKDKLY QTPRLNDVLY LHYQGFQCIE SLEEYTELKC
LWLECNAISE IQGLEKLSKL KCLFLQNNLI TKIENLDPCR ELDTLNLSSN HIRKIQNIGT
NVLPVLNTLT ISSNYLKDSE SLSDLIQCKT LSVLDLSNNR IDDILIVKIF EQMLNLKVLV
LQGNPVVSRL PQYRKTLILA CKELTYLDSR PVFPRDRACA EAWKRDGYEG ERKENNRWNR
AERRKTRESI NCTIRMRNSH RPPDQQDPLL RSSDSEDDTC AETARKKVAL ENGCVDDLWE
EVSGEQPISE DGTNSSSSLE DNDGTSSQDD LIAEKLSNRR TLEGRPTVLY ETEVSNVKSA
NNDINIFEEG VGKASQIIIE DKPVTKIKLI NEPSCMNDKS AMNCTSMGPV VKENDFDEDA
EIKNVDDMVP CQNLIKSNED INSEFGFSSK LKQDFDQTCT ALRNDAECKE FDEELTIKES
ESKPFNEMYD IFAAEDDKLN ETLDLNLNET TCPHHVHDNF FSEQNKMPSF YEEDIMINLL
PKTKEKTLLE KDSIIENEKC AHDLDEMGRQ MEEDLAELRQ STQNLIGISI DETARTDSET
DEEDLIAQQD PYSPLLKQQF KDRRMKIMLT EETKAQEESL SDRNIALSNE SDQDDKQDQL
FAKILDDATE NIPKRIFGTG CDALSSVWPQ EECLLQLTLP ENKESPAQED TFKKSITNST
SFEKANEICV RMDQKMAEEE EALGKLLHDL EGEINNVYDI NTKVKYEENT STNESDAVSI
CTSLLDDIIV GLTFDEVLCH EKPKSFEFGL IESDEEFSYS FEPKLEKLVP PALEDPARGK
SLRECLDTFS DFVSSMADPK LPVMLGRNPT SGVEKIRAAQ ELLKTKNLSD LYKDTAESLN
SQVAKEIEKR KRRVAASATR CFNQRDKYDD TLELVENRLM IVKKDSGDLE ELPPPPPLIS
DTESEDDNPT EDNYTPGNEV HRHGTKTQDS KEHLMSNLLN QNQDDIIEGK KNDSAEDEFY
SLEAMTTFGN LDAEFFQKLD LQKVNASEDS EPAINCMRSY NELQAYMKSG SLNTQLNSEE
AEMLQTMCSR VASDVDKPKS QNSKEEEEDD LLKKMVLRMK EYEEREHQLQ LISHETSSEL
GPIKLSIGGS KLFEQNTKIP ESVLVHSENE PTRNNKPINT KNTNDKESSD IMEKNGDPSV
TTSFKTSHKS IDDDIQSDVS TDYESGEEVV VVEPPTLSEA VLKSFYSDEF EADSKMVHEL
EEATRRNLRR YNPNIMHIST SNHPFSTNNI LPTKTSTSEL SEGAKAKWAK IAERLHEFLD
PETIANLNKE QFGESDECED SQDDNITDIT VEETNLKKDT KLVVLEEYNN THTCDGNSCP
SKLDDSDQFA SLKGFEKIKE TPTHNMGLSS VMSASQNKTS ETLPNEIGNK ITDFSSFKTS
STDLINSEGV KTEQIQCNLQ ILSDEGDVVV EELSVNAQVS SFK
