DAAF1_MOUSE
ID DAAF1_MOUSE Reviewed; 634 AA.
AC Q9D2H9; Q9CVS9;
DT 18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Dynein axonemal assembly factor 1;
DE AltName: Full=Leucine-rich repeat-containing protein 50;
GN Name=Dnaaf1; Synonyms=Lrrc50;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-462; SER-465 AND SER-488, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Cilium-specific protein required for the stability of the
CC ciliary architecture. Plays a role in cytoplasmic preassembly of dynein
CC arms (By similarity). Involved in regulation of microtubule-based cilia
CC and actin-based brush border microvilli (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DNAAF1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK006656; BAB24692.1; -; mRNA.
DR EMBL; AK019633; BAB31818.1; -; mRNA.
DR EMBL; BC050751; AAH50751.1; -; mRNA.
DR CCDS; CCDS22709.1; -.
DR RefSeq; NP_080924.1; NM_026648.4.
DR AlphaFoldDB; Q9D2H9; -.
DR SMR; Q9D2H9; -.
DR IntAct; Q9D2H9; 2.
DR MINT; Q9D2H9; -.
DR STRING; 10090.ENSMUSP00000090790; -.
DR iPTMnet; Q9D2H9; -.
DR PhosphoSitePlus; Q9D2H9; -.
DR PaxDb; Q9D2H9; -.
DR PRIDE; Q9D2H9; -.
DR ProteomicsDB; 279230; -.
DR Antibodypedia; 30549; 190 antibodies from 15 providers.
DR DNASU; 68270; -.
DR Ensembl; ENSMUST00000093100; ENSMUSP00000090790; ENSMUSG00000031831.
DR GeneID; 68270; -.
DR KEGG; mmu:68270; -.
DR UCSC; uc009npw.1; mouse.
DR CTD; 123872; -.
DR MGI; MGI:1915520; Dnaaf1.
DR VEuPathDB; HostDB:ENSMUSG00000031831; -.
DR eggNOG; ENOG502QQFE; Eukaryota.
DR GeneTree; ENSGT00940000158494; -.
DR HOGENOM; CLU_027574_0_0_1; -.
DR InParanoid; Q9D2H9; -.
DR OMA; IAHNHLE; -.
DR OrthoDB; 891307at2759; -.
DR PhylomeDB; Q9D2H9; -.
DR TreeFam; TF315818; -.
DR BioGRID-ORCS; 68270; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Dnaaf1; mouse.
DR PRO; PR:Q9D2H9; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9D2H9; protein.
DR Bgee; ENSMUSG00000031831; Expressed in seminiferous tubule of testis and 54 other tissues.
DR Genevisible; Q9D2H9; MM.
DR GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0070840; F:dynein complex binding; ISS:UniProtKB.
DR GO; GO:0070286; P:axonemal dynein complex assembly; ISO:MGI.
DR GO; GO:0035082; P:axoneme assembly; IBA:GO_Central.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0003341; P:cilium movement; ISO:MGI.
DR GO; GO:0071907; P:determination of digestive tract left/right asymmetry; ISO:MGI.
DR GO; GO:0071910; P:determination of liver left/right asymmetry; ISO:MGI.
DR GO; GO:0035469; P:determination of pancreatic left/right asymmetry; ISO:MGI.
DR GO; GO:0001947; P:heart looping; ISO:MGI.
DR GO; GO:0036159; P:inner dynein arm assembly; ISO:MGI.
DR GO; GO:0060972; P:left/right pattern formation; ISO:MGI.
DR GO; GO:0030324; P:lung development; ISO:MGI.
DR GO; GO:0044458; P:motile cilium assembly; ISO:MGI.
DR GO; GO:0036158; P:outer dynein arm assembly; ISO:MGI.
DR GO; GO:0003356; P:regulation of cilium beat frequency; ISO:MGI.
DR Gene3D; 3.80.10.10; -; 2.
DR InterPro; IPR027734; DNAAF1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR PANTHER; PTHR45973:SF19; PTHR45973:SF19; 1.
DR PROSITE; PS51450; LRR; 6.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Leucine-rich repeat; Phosphoprotein;
KW Reference proteome; Repeat.
FT CHAIN 1..634
FT /note="Dynein axonemal assembly factor 1"
FT /id="PRO_0000232890"
FT REPEAT 101..123
FT /note="LRR 1"
FT REPEAT 124..145
FT /note="LRR 2"
FT REPEAT 146..167
FT /note="LRR 3"
FT REPEAT 168..189
FT /note="LRR 4"
FT REPEAT 190..211
FT /note="LRR 5"
FT REPEAT 215..236
FT /note="LRR 6"
FT DOMAIN 249..288
FT /note="LRRCT"
FT REGION 1..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 326..358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 481..505
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 559..634
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..66
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 609..625
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 349
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6AYH9"
FT MOD_RES 462
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 465
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 488
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 634 AA; 69704 MW; 4B174F328D433754 CRC64;
MHPEVSEPPV DSVAEPSLEE SAGDHGDAGP GIRKEEISET KETCAGPCTT SCPSQQQPSG
DNGSEGFCTH SRDDREDRGP RMTKQFLQKL CKQHKLYVTP ALNDTLYLHF KGFDRIENLE
EYTGLRCLWL ECNGIQRIEN LQAQSELRCL FLQVNLLHKI ENLEPLQKLD ALNLSNNYIK
TIENLSCLPV LNTLQMAHNR LETVADIEHL RECLRLCVLD LSHNALSDPE ILSVLESMPC
LRVLNLMGNP VTKHIPNYRR TVTVRLKHLT YLDDRPVFPK DRACAEAWAR GGYAAEKEER
RQWESREHKK ITDSLEALAM IKRRAEERKK ARDRGETPLP DSEGSIPTSP EAEEKQPMGE
IQKKMELFVE ESFEAKDELF PETPGGEKEL HVVVVNGAVE NPDLSGSLAH NQTPVVVTPE
ESTSPVAATD GARTEDIEAV AVEIKERLFI DDLPDLEDAE GTDVSVEDQI KETDIPRIQA
ISSLSDDSDP ELDELSLSTS EATPTGATGA LSHIFAISKG PSTAATVPFT DICKPIATTD
LESQGQDCGA AASRPLIQEL NDEPAEEAAN QPLPPQTCAS DPALAHPSED GDSDSQLPAA
TLLGDGAEDE AESSVHPKEP STRVGLEDIE FGLD
