ID   DAAF3_CHLRE             Reviewed;         710 AA.
AC   F5A894;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 17.
DE   RecName: Full=Dynein axonemal assembly factor 3 homolog;
DE   AltName: Full=Dynein assembly blocked protein 1;
DE   AltName: Full=Paralyzed flagella protein 22;
GN   Name=DAB1; Synonyms=PF22;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=CC-503;
RX   PubMed=22387996; DOI=10.1038/ng.1106;
RA   Mitchison H.M., Schmidts M., Loges N.T., Freshour J., Dritsoula A.,
RA   Hirst R.A., O'Callaghan C., Blau H., Al Dabbagh M., Olbrich H.,
RA   Beales P.L., Yagi T., Mussaffi H., Chung E.M., Omran H., Mitchell D.R.;
RT   "Mutations in axonemal dynein assembly factor DNAAF3 cause primary ciliary
RT   dyskinesia.";
RL   Nat. Genet. 44:381-389(2012).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=429335; DOI=10.1016/s0021-9258(17)30186-2;
RA   Huang B., Piperno G., Luck D.J.;
RT   "Paralyzed flagella mutants of Chlamydomonas reinhardtii. Defective for
RT   axonemal doublet microtubule arms.";
RL   J. Biol. Chem. 254:3091-3099(1979).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=1387875; DOI=10.1083/jcb.118.6.1455;
RA   Piperno G., Mead K., Shestak W.;
RT   "The inner dynein arms I2 interact with a 'dynein regulatory complex' in
RT   Chlamydomonas flagella.";
RL   J. Cell Biol. 118:1455-1463(1992).
CC   -!- FUNCTION: Required for the assembly of axonemal inner and outer dynein
CC       arms. Involved in preassembly of dyneins into complexes before their
CC       transport into cilia. {ECO:0000269|PubMed:22387996}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22387996}.
CC   -!- DISRUPTION PHENOTYPE: Nonmotile flagella, with paralyzed half-length
CC       flagella and disrupted outer dynein arms assembly.
CC       {ECO:0000269|PubMed:22387996}.
CC   -!- SIMILARITY: Belongs to the DNAAF3 family. {ECO:0000305}.
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DR   EMBL; HQ424432; AEC04845.1; -; Genomic_DNA.
DR   AlphaFoldDB; F5A894; -.
DR   PRIDE; F5A894; -.
DR   EnsemblPlants; PNW87771; PNW87771; CHLRE_01g001657v5.
DR   Gramene; PNW87771; PNW87771; CHLRE_01g001657v5.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0070286; P:axonemal dynein complex assembly; IMP:UniProtKB.
DR   InterPro; IPR039304; DNAAF3.
DR   InterPro; IPR028235; DNAAF3_C.
DR   InterPro; IPR027974; DUF4470.
DR   PANTHER; PTHR22118; PTHR22118; 2.
DR   Pfam; PF14737; DUF4470; 1.
DR   Pfam; PF14740; DUF4471; 1.
PE   3: Inferred from homology;
KW   Cilium biogenesis/degradation; Cytoplasm.
FT   CHAIN           1..710
FT                   /note="Dynein axonemal assembly factor 3 homolog"
FT                   /id="PRO_0000416897"
FT   REGION          403..487
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   710 AA;  72736 MW;  CF7039A32B29F26E CRC64;
     MDEHNVHHFW GISPAVDLGT LLPTGDGAAD LPVDQPVRFL QVAPYDARHT LTTLSRACRH
     PALMRQLPPG QPACRLYVWE DSPEGLARHV LLAAVLLDGG VPAAQRGQLL LELHGNAVLR
     RRAAEYLDEK ARQLESLFVG LAAGQPPPAG PEAEARGLAA LAALLDLSLL KFQEKDLIVE
     ALQRWRLPNP TSSTSTSAAS ASAAAAPYDM VAAWDGRCRK VYGERYDFRR NMVDWDYHMR
     LQPAGTPGCD PASGSIIHFH HFRHWRLHGV AHELRDSAYN SANRCRGEGG VGEFKDRTGR
     DVGRSVSAWG FWADVLNSPY HAFGTACEQP EFYRITNKQF VRTAVDVAEH NIAALLHELR
     TGRRLELGEG QEAHRAQAAR GPTTLEDLTA AAEEAAAAAS GAGAEAGAGA GPGGEAAAGA
     SSSSGKEEAA AAAAAGKEQG QGEGQGEDWT AGSGSGAPGA GTGQAVAEGR EGPGGPQDSD
     PAAAASTAAP AAAAAAASSS STSVPTYSSG GRAQVAKAMA AAAAAAGAGA GAGAEASSSG
     AASAASAAPT AGASSGAADG KAAEAAAAPL DEAAREQMAA EEAAVRAAEA ALDAAARQRA
     GRFRLVLVTG DLAKTLTGRA KYAGAFSGLS LGHRHTHMLE PQYKLAAAAA PGARLVAENA
     RHVLQLSQEQ AALFAAKMDE LAAAGGWRPL PAAQRPPGIT EAAAVYVRAA