ID   DAAF3_DANRE             Reviewed;         469 AA.
AC   F1Q7Z7; E9QIE9; F1QTP0;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2012, sequence version 2.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Dynein axonemal assembly factor 3;
GN   Name=dnaaf3; ORFNames=si:dkey-224b4.3;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX   PubMed=22387996; DOI=10.1038/ng.1106;
RA   Mitchison H.M., Schmidts M., Loges N.T., Freshour J., Dritsoula A.,
RA   Hirst R.A., O'Callaghan C., Blau H., Al Dabbagh M., Olbrich H.,
RA   Beales P.L., Yagi T., Mussaffi H., Chung E.M., Omran H., Mitchell D.R.;
RT   "Mutations in axonemal dynein assembly factor DNAAF3 cause primary ciliary
RT   dyskinesia.";
RL   Nat. Genet. 44:381-389(2012).
CC   -!- FUNCTION: Required for the assembly of axonemal inner and outer dynein
CC       arms. Involved in preassembly of dyneins into complexes before their
CC       transport into cilia. {ECO:0000269|PubMed:22387996}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Dynein axonemal particle
CC       {ECO:0000250|UniProtKB:Q32NQ7}.
CC   -!- TISSUE SPECIFICITY: Mainly expressed in cell types that express
CC       axonemal dyneins. {ECO:0000269|PubMed:22387996}.
CC   -!- DISRUPTION PHENOTYPE: Static olfactory placode cilia; reduced movement
CC       of debris in the olfactory placode. The morphology and number of cilia
CC       in the olfactory placode is unaffected, but an abnormal ultrastructure
CC       in these cilia is observed, including reduced or missing outer and
CC       inner dynein arms. {ECO:0000269|PubMed:22387996}.
CC   -!- SIMILARITY: Belongs to the DNAAF3 family. {ECO:0000305}.
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DR   EMBL; CR954298; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; F1Q7Z7; -.
DR   STRING; 7955.ENSDARP00000118558; -.
DR   PaxDb; F1Q7Z7; -.
DR   PRIDE; F1Q7Z7; -.
DR   Ensembl; ENSDART00000137777; ENSDARP00000118558; ENSDARG00000092662.
DR   ZFIN; ZDB-GENE-091204-296; dnaaf3.
DR   eggNOG; ENOG502QT97; Eukaryota.
DR   GeneTree; ENSGT00390000002069; -.
DR   HOGENOM; CLU_024420_2_1_1; -.
DR   InParanoid; F1Q7Z7; -.
DR   OMA; PEYEQCK; -.
DR   OrthoDB; 681465at2759; -.
DR   PhylomeDB; F1Q7Z7; -.
DR   TreeFam; TF323981; -.
DR   PRO; PR:F1Q7Z7; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 24.
DR   Bgee; ENSDARG00000092662; Expressed in testis and 6 other tissues.
DR   GO; GO:0120293; C:dynein axonemal particle; ISS:UniProtKB.
DR   GO; GO:0070286; P:axonemal dynein complex assembly; IMP:ZFIN.
DR   GO; GO:0003341; P:cilium movement; IMP:ZFIN.
DR   GO; GO:0044458; P:motile cilium assembly; IMP:UniProtKB.
DR   InterPro; IPR039304; DNAAF3.
DR   InterPro; IPR028235; DNAAF3_C.
DR   InterPro; IPR027974; DUF4470.
DR   PANTHER; PTHR22118; PTHR22118; 1.
DR   Pfam; PF14737; DUF4470; 1.
DR   Pfam; PF14740; DUF4471; 1.
PE   2: Evidence at transcript level;
KW   Cilium biogenesis/degradation; Cytoplasm; Reference proteome.
FT   CHAIN           1..469
FT                   /note="Dynein axonemal assembly factor 3"
FT                   /id="PRO_0000416895"
FT   REGION          313..356
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        317..356
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   469 AA;  52577 MW;  57FC852FA01E408C CRC64;
     MSAGRTFEGA GCVTWWGFGP ARDLLNSDTH KVRLQEELNV LLVGSGDPRH ILKTITGLTH
     SDTLHVWVIE NSMEVIARQL LLLYISLLPP DKMSVHKKTE VFLEVFGNLE IRKETEESVK
     KAAAQLSISI TYSLSSDSLS HSCLDTSLLK FKERDELVRI FKLWERPPSA PASVSKVWDA
     RVRQHLGSRY DSRQGAFDWD LNMKLHQRGC GVINKHQYAK WRETGVTFEM REGLYQTANQ
     SLLSTRVFNH RGNGVALRGY WGDIVSSPYL SFGIETENKE LLKTQNNHYV KTAQDISEVN
     LLELFECLAA RGRSPLNEDP PNTSSSCCQS TESRKTEENS QSDPSASQTQ PVEHSPTQEL
     DLLNVNGVKV SFLSPDSLSK LPLKSKYRNL FNTIFCSASM VHQLDSALRE IAAPDAALVI
     ELATFLLDLS KEQVSGFAVK VKEIAEESGF TPAHDQNSDK YAVFTQKNN