DAAF5_DROME
ID DAAF5_DROME Reviewed; 845 AA.
AC Q8INF7;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Dynein axonemal assembly factor 5 {ECO:0000305};
DE AltName: Full=HEAT repeat-containing protein 2 homolog {ECO:0000305};
GN Name=HEATR2 {ECO:0000312|FlyBase:FBgn0051320};
GN ORFNames=CG31320 {ECO:0000312|FlyBase:FBgn0051320};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Ovary;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE, AND TISSUE
RP SPECIFICITY.
RX PubMed=25232951; DOI=10.1371/journal.pgen.1004577;
RA Diggle C.P., Moore D.J., Mali G., zur Lage P., Ait-Lounis A., Schmidts M.,
RA Shoemark A., Garcia Munoz A., Halachev M.R., Gautier P., Yeyati P.L.,
RA Bonthron D.T., Carr I.M., Hayward B., Markham A.F., Hope J.E.,
RA von Kriegsheim A., Mitchison H.M., Jackson I.J., Durand B., Reith W.,
RA Sheridan E., Jarman A.P., Mill P.;
RT "HEATR2 plays a conserved role in assembly of the ciliary motile
RT apparatus.";
RL PLoS Genet. 10:E1004577-E1004577(2014).
CC -!- FUNCTION: Cytoplasmic protein involved in the delivery of the dynein
CC machinery to the motile cilium. It is required for the assembly of the
CC axonemal dynein inner and outer arms, two structures attached to the
CC peripheral outer doublet A microtubule of the axoneme, that play a
CC crucial role in cilium motility. {ECO:0000269|PubMed:25232951}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:25232951}. Dynein
CC axonemal particle {ECO:0000250|UniProtKB:Q86Y56}. Note=Observed only in
CC the cytoplasm of ciliated cells and absent from cilia.
CC {ECO:0000269|PubMed:25232951}.
CC -!- TISSUE SPECIFICITY: Expressed in testis. {ECO:0000305|PubMed:25232951}.
CC -!- DEVELOPMENTAL STAGE: Expressed in chordonotal neurons during their
CC differentiation, after neuronal specification but before cilium
CC formation. Also expressed in the non-ciliated mesoderm of stage 12
CC embryos. {ECO:0000269|PubMed:25232951}.
CC -!- DISRUPTION PHENOTYPE: Homozygous deletion-mutant flies are not viable.
CC The larvae are smaller but do not display obvious morphological
CC phenotype. RNAi-mediated knockdown in sensory neurons affects flies
CC movement coordination and impairs hearing. Testis-specific RNAi-
CC mediated knockdown does not alter spermatogenesis and in particular the
CC formation of sperm axonemes but it impairs sperm motility.
CC {ECO:0000269|PubMed:25232951}.
CC -!- SIMILARITY: Belongs to the DNAAF5 family. {ECO:0000305}.
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DR EMBL; AE014297; AAN13608.1; -; Genomic_DNA.
DR EMBL; BT006021; AAO74704.1; -; mRNA.
DR RefSeq; NP_731905.1; NM_169581.2.
DR AlphaFoldDB; Q8INF7; -.
DR STRING; 7227.FBpp0082402; -.
DR PaxDb; Q8INF7; -.
DR PRIDE; Q8INF7; -.
DR DNASU; 318680; -.
DR EnsemblMetazoa; FBtr0082943; FBpp0082402; FBgn0051320.
DR GeneID; 318680; -.
DR KEGG; dme:Dmel_CG31320; -.
DR UCSC; CG31320-RA; d. melanogaster.
DR CTD; 318680; -.
DR FlyBase; FBgn0051320; HEATR2.
DR VEuPathDB; VectorBase:FBgn0051320; -.
DR eggNOG; ENOG502QRXT; Eukaryota.
DR GeneTree; ENSGT00390000005666; -.
DR HOGENOM; CLU_010823_1_0_1; -.
DR InParanoid; Q8INF7; -.
DR OMA; RKHMGPL; -.
DR OrthoDB; 123510at2759; -.
DR PhylomeDB; Q8INF7; -.
DR BioGRID-ORCS; 318680; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 318680; -.
DR PRO; PR:Q8INF7; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0051320; Expressed in testis and 33 other tissues.
DR Genevisible; Q8INF7; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0120293; C:dynein axonemal particle; ISS:UniProtKB.
DR GO; GO:0045505; F:dynein intermediate chain binding; ISS:FlyBase.
DR GO; GO:0008344; P:adult locomotory behavior; IMP:FlyBase.
DR GO; GO:0003341; P:cilium movement; ISS:UniProtKB.
DR GO; GO:0060294; P:cilium movement involved in cell motility; IMP:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:UniProtKB.
DR GO; GO:0036159; P:inner dynein arm assembly; IMP:UniProtKB.
DR GO; GO:0036158; P:outer dynein arm assembly; IMP:UniProtKB.
DR GO; GO:0007605; P:sensory perception of sound; IMP:UniProtKB.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 2: Evidence at transcript level;
KW Cilium biogenesis/degradation; Cytoplasm; Reference proteome; Repeat.
FT CHAIN 1..845
FT /note="Dynein axonemal assembly factor 5"
FT /id="PRO_0000431657"
FT REPEAT 47..84
FT /note="HEAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 138..175
FT /note="HEAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 180..217
FT /note="HEAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 260..297
FT /note="HEAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 332..369
FT /note="HEAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 523..561
FT /note="HEAT 6"
FT /evidence="ECO:0000255"
FT REPEAT 674..715
FT /note="HEAT 7"
FT /evidence="ECO:0000255"
FT REPEAT 766..803
FT /note="HEAT 8"
FT /evidence="ECO:0000255"
SQ SEQUENCE 845 AA; 96290 MW; 38BEC5CA2852DDC0 CRC64;
MGFDIDSQKI CSDLESSDRR LKTTVLDELL DKCKEATNSE SADKIADVFD KLYLHLLKCY
EDRFESVRSK AIQVVSAFLS SLPPTDFHLM NVVSTLAERM GKAETVEPSE EIRLLYIAQL
NLMICLYEKM ENVGVFRECY PLVVKILIKS IKDDYPVVQR EGCSAVVTLS RLADTQEFRP
FTESILLPLY TMLNHKHAQA RISAIQAIAR LSLHMDASGD AMRRLFNEVS PLLMDTMPLV
RREVGQMGIL MLMELLDRYS FFERILPLVL CCLKDESPEV LNHIYPQWLK CGIQYFNENE
AELSQQEISD LPVENYPKDV KRPTIGCRGL VQRSLRLLQL ITRETSDWKD NVRLHALKLL
YQFVLHAEAA MTAKFFEIYG DLAHACIDHV AEVNAEAAKV ADLMGRLLSY DAWIDHGFDG
LERNARESYM RCFYHMFTAS LGGTYEQLMR LAKLLRCTDY SHTLKPGFQH YILKLLNTIV
DKTLKINAGQ NELEDLYESV YVCAIKVMAL SSSLENVGDE DVNFGQTLIE KMVKLLNTSV
PKIHERWFHL ALQDVINLDA ALEDNAEPVM LLNGLINMCH IRATYVHDLI EKVKIVFQHC
CDSAQVKIFS SLSLATLFWS KTMNVERESS TQMLSEFVSQ IVEPYLTWKA GSNAEAMRSL
AMATLCALAQ GAESESVEVL PSLAKYMPSL LEDRNVTTRH YAIKAVVYFR EMSVEDLKPL
AYATMQRMDD PSAGIRILAA LAMGKLKPKF AETDTEENYE KEVWDAIVKR AMDLLLLYHE
SPEKDMRAAV AVTLKVLAKS HPEAWEERYQ RALPMAQKKD LLTELYDKLT INEDSHTEVT
SASQD
