ID   DAAF5_XENLA             Reviewed;         839 AA.
AC   A0JMW2;
DT   07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 1.
DT   03-AUG-2022, entry version 58.
DE   RecName: Full=Dynein axonemal assembly factor 5 {ECO:0000305};
DE   AltName: Full=HEAT repeat-containing protein 2;
GN   Name=dnaaf5 {ECO:0000312|Xenbase:XB-GENE-5902240}; Synonyms=heatr2;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355 {ECO:0000312|EMBL:AAI26028.1};
RN   [1] {ECO:0000312|EMBL:AAI26028.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Ovary {ECO:0000312|EMBL:AAI26028.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=30561330; DOI=10.7554/elife.38497;
RA   Huizar R.L., Lee C., Boulgakov A.A., Horani A., Tu F., Marcotte E.M.,
RA   Brody S.L., Wallingford J.B.;
RT   "A liquid-like organelle at the root of motile ciliopathy.";
RL   Elife 7:0-0(2018).
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=33263282; DOI=10.7554/elife.58662;
RA   Lee C., Cox R.M., Papoulas O., Horani A., Drew K., Devitt C.C., Brody S.L.,
RA   Marcotte E.M., Wallingford J.B.;
RT   "Functional partitioning of a liquid-like organelle during assembly of
RT   axonemal dyneins.";
RL   Elife 9:0-0(2020).
CC   -!- FUNCTION: Cytoplasmic protein involved in the delivery of the dynein
CC       machinery to the motile cilium. It is required for the assembly of the
CC       axonemal dynein inner and outer arms, two structures attached to the
CC       peripheral outer doublet A microtubule of the axoneme, that play a
CC       crucial role in cilium motility. {ECO:0000250|UniProtKB:Q86Y56}.
CC   -!- SUBUNIT: Interacts with DNAI2; probably involved in outer arm dynein
CC       assembly. {ECO:0000250|UniProtKB:Q86Y56}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q86Y56}. Dynein
CC       axonemal particle {ECO:0000269|PubMed:30561330,
CC       ECO:0000269|PubMed:33263282}. Note=Observed only in the cytoplasm of
CC       ciliated cells and absent from cilia. {ECO:0000250|UniProtKB:Q86Y56}.
CC   -!- SIMILARITY: Belongs to the DNAAF5 family. {ECO:0000305}.
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DR   EMBL; BC126027; AAI26028.1; -; mRNA.
DR   RefSeq; NP_001090507.1; NM_001097038.1.
DR   AlphaFoldDB; A0JMW2; -.
DR   DNASU; 779420; -.
DR   GeneID; 779420; -.
DR   KEGG; xla:779420; -.
DR   CTD; 779420; -.
DR   Xenbase; XB-GENE-5902240; dnaaf5.L.
DR   OrthoDB; 123510at2759; -.
DR   Proteomes; UP000186698; Chromosome 9_10L.
DR   Bgee; 779420; Expressed in testis and 1 other tissue.
DR   GO; GO:0120293; C:dynein axonemal particle; IDA:UniProtKB.
DR   GO; GO:0030030; P:cell projection organization; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.10.10; -; 3.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR000357; HEAT.
DR   InterPro; IPR021133; HEAT_type_2.
DR   Pfam; PF02985; HEAT; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
DR   PROSITE; PS50077; HEAT_REPEAT; 1.
PE   2: Evidence at transcript level;
KW   Cilium biogenesis/degradation; Cytoplasm; Reference proteome; Repeat.
FT   CHAIN           1..839
FT                   /note="Dynein axonemal assembly factor 5"
FT                   /id="PRO_0000452440"
FT   REPEAT          10..48
FT                   /note="HEAT 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          54..92
FT                   /note="HEAT 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          94..137
FT                   /note="HEAT 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          140..178
FT                   /note="HEAT 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          181..219
FT                   /note="HEAT 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          221..257
FT                   /note="HEAT 7"
FT                   /evidence="ECO:0000255"
FT   REPEAT          259..297
FT                   /note="HEAT 8"
FT                   /evidence="ECO:0000255"
FT   REPEAT          578..617
FT                   /note="HEAT 9"
FT                   /evidence="ECO:0000255"
FT   REPEAT          675..713
FT                   /note="HEAT 10"
FT                   /evidence="ECO:0000255"
FT   REPEAT          717..755
FT                   /note="HEAT 11"
FT                   /evidence="ECO:0000255"
FT   REPEAT          723..761
FT                   /note="HEAT 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00103"
SQ   SEQUENCE   839 AA;  95182 MW;  320C47177D4B7C3C CRC64;
     MAAEGEQRAT SDVTKALARH LNCLNDENKM IRRRALAAIQ KEAADEKLAS AVLQHVFLEL
     LKPLLRCLSD PMEKCRELSI QIIVYCVSHV PRPEEALPYL MPALTQRLGQ QQLVELSEEL
     RLAMVELLTL LVEVCGKKLA PYLDEMIQIF QRTMVDPFPD VKKESCKCAS NYATCIPEHF
     HMQAESLIKP LMQTISHQHS KVRVAVIQTT GTVIQYSSGK SVDDVLSHLA QRLFDDSVQV
     RQAVTVVVGD WLLKLQDRYS FFHKLIPLLL SSTTDEIPEI RKLALDYWEK IGSQWEKENE
     EDLKDKLDFS APCPSYYPAG VQRPGLGCRE LIFRNLSKIL PAISRDVTNW VAGTRIKSSQ
     LLAVLLLHGE DHITQHMELL LSTLYHSCLD EENQVVINSV KSAELIGTFV NPEVFLKLIL
     SALQKSPLAS HLMTLAACIR GCSREMLKTH LTKITSVISQ SDVCQGSERV LYQEQLLCCI
     QSLIDVCQQD CTEVSLQCMN VLITILASPA SESLHKKVQD TLLSLSEVQG LNGPHDLYRQ
     HMQQLLDWVS QSHNHWTSYS VEQRQFEVIA TESGPVMGET LHLFVPILKM CLQPTREPQM
     RLKLFTMLSK LLLKASETVN SQGQLHRYSE SFINDMIVPN LKWQAGRTAG AIRTVAVSCL
     WVLLQSETLS QQEILQVEDN LMPRVITTLE EDSKMCRLMS CCIITALLST CERQLRPDKL
     NKIYPELLKR LDDASDEVRV AAAKTLYQWF KCITDEYERT TYKGHLEFLY RGLLVHLDDP
     DASLQLIVLD LLKEGSAVCP SLLVQEIEAV RHKHRSPAYC DQLLHYIQKY TSPTPTECT