DACA_LISM4
ID DACA_LISM4 Reviewed; 273 AA.
AC A0A0H3GM48;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 16-SEP-2015, sequence version 1.
DT 25-MAY-2022, entry version 32.
DE RecName: Full=Diadenylate cyclase {ECO:0000255|HAMAP-Rule:MF_01499};
DE Short=DAC {ECO:0000255|HAMAP-Rule:MF_01499};
DE EC=2.7.7.85 {ECO:0000255|HAMAP-Rule:MF_01499};
DE AltName: Full=Cyclic-di-AMP synthase {ECO:0000255|HAMAP-Rule:MF_01499};
DE Short=c-di-AMP synthase {ECO:0000255|HAMAP-Rule:MF_01499};
GN Name=dacA {ECO:0000255|HAMAP-Rule:MF_01499}; OrderedLocusNames=LMRG_01274;
OS Listeria monocytogenes serotype 1/2a (strain 10403S).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria.
OX NCBI_TaxID=393133;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=10403S;
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Borowsky M., Borodovsky M., Young S.K., Zeng Q., Koehrsen M.,
RA Fitzgerald M., Wiedmann M., Swaminathan B., Lauer P., Portnoy D.,
RA Cossart P., Buchrieser C., Higgins D., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A., Borenstein D., Brown A., Chapman S.B., Chen Z.,
RA Dunbar C.D., Engels R., Freedman E., Gearin G., Gellesch M., Goldberg J.,
RA Griggs A., Gujja S., Heilman E., Heiman D., Howarth C., Jen D., Larson L.,
RA Lui A., MacDonald J., Mehta T., Montmayeur A., Neiman D., Park D.,
RA Pearson M., Priest M., Richards J., Roberts A., Saif S., Shea T.,
RA Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C.,
RA Haas B., Nusbaum C., Birren B.;
RT "The genome sequence of Listeria monocytogenes strain 10403S.";
RL Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=10403S;
RX PubMed=23716572; DOI=10.1128/mbio.00282-13;
RA Witte C.E., Whiteley A.T., Burke T.P., Sauer J.D., Portnoy D.A.,
RA Woodward J.J.;
RT "Cyclic di-AMP is critical for Listeria monocytogenes growth, cell wall
RT homeostasis, and establishment of infection.";
RL MBio 4:E00282-E00282(2013).
RN [3]
RP DISRUPTION PHENOTYPE.
RC STRAIN=10403S;
RX PubMed=25965978; DOI=10.1021/jacs.5b00275;
RA Kellenberger C.A., Chen C., Whiteley A.T., Portnoy D.A., Hammond M.C.;
RT "RNA-based fluorescent biosensors for live cell imaging of second messenger
RT cyclic di-AMP.";
RL J. Am. Chem. Soc. 137:6432-6435(2015).
CC -!- FUNCTION: Catalyzes the condensation of 2 ATP molecules into cyclic di-
CC AMP (c-di-AMP), a signaling compound secreted into the host's cytosol
CC where it triggers the cytosolic surveillance pathway (CSP), a host
CC pathway of innate immunity characterized by expression of beta
CC interferon (IFN-beta) and coregulated genes (Probable). Overexpression
CC increases export of c-di-AMP (PubMed:23716572). c-di-AMP is a second
CC messenger that mediates growth, cell wall stability and virulence
CC (Probable). {ECO:0000269|PubMed:23716572, ECO:0000305|PubMed:23716572}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 ATP = 3',3'-c-di-AMP + 2 diphosphate; Xref=Rhea:RHEA:35655,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:71500; EC=2.7.7.85;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01499};
CC -!- SUBUNIT: Probably a homodimer. {ECO:0000255|HAMAP-Rule:MF_01499}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01499};
CC Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01499}.
CC -!- DISRUPTION PHENOTYPE: Essential, it cannot be deleted; in depletion
CC experiments doubling time increases from 44 to 84 minutes, cells have
CC decreased export of c-di-AMP, increased susceptibility to the
CC antibiotic cefuroxime, a weakened cell wall, 10-fold lower growth in
CC host macrophages, are about 10(3) less virulent in mice and more
CC susceptible to in-host bacteriolysis (PubMed:23716572). In a viable
CC dacA-relA-relP-relQ deletion mutant about 2-fold decreased
CC intracellular levels of c-di-AMP (PubMed:25965978).
CC {ECO:0000269|PubMed:23716572, ECO:0000269|PubMed:25965978}.
CC -!- SIMILARITY: Belongs to the adenylate cyclase family. DacA/CdaA
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01499}.
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DR EMBL; CP002002; AEO07105.1; -; Genomic_DNA.
DR RefSeq; WP_003722380.1; NC_017544.1.
DR AlphaFoldDB; A0A0H3GM48; -.
DR SMR; A0A0H3GM48; -.
DR EnsemblBacteria; AEO07105; AEO07105; LMRG_01274.
DR GeneID; 67410225; -.
DR KEGG; lmt:LMRG_01274; -.
DR HOGENOM; CLU_038561_0_1_9; -.
DR OMA; ILWQGEL; -.
DR Proteomes; UP000001288; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004016; F:adenylate cyclase activity; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0106408; F:diadenylate cyclase activity; IEA:UniProtKB-EC.
DR GO; GO:0006171; P:cAMP biosynthetic process; IEA:InterPro.
DR GO; GO:0019932; P:second-messenger-mediated signaling; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.1700.10; -; 1.
DR HAMAP; MF_01499; DacA; 1.
DR InterPro; IPR014046; C-di-AMP_synthase.
DR InterPro; IPR034701; CdaA.
DR InterPro; IPR045585; CdaA_N.
DR InterPro; IPR036888; DNA_integrity_DisA_N_sf.
DR InterPro; IPR003390; DNA_integrity_scan_DisA_N.
DR Pfam; PF19293; CdaA_N; 1.
DR Pfam; PF02457; DAC; 1.
DR PIRSF; PIRSF004793; UCP004793; 1.
DR SUPFAM; SSF143597; SSF143597; 1.
DR TIGRFAMs; TIGR00159; TIGR00159; 1.
DR PROSITE; PS51794; DAC; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW Nucleotidyltransferase; Transferase; Transmembrane; Transmembrane helix.
FT CHAIN 1..273
FT /note="Diadenylate cyclase"
FT /id="PRO_0000436051"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01499"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01499"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01499"
FT DOMAIN 82..242
FT /note="DAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01130"
SQ SEQUENCE 273 AA; 30445 MW; 878951A75FB06D88 CRC64;
MDFSNMSILH YLANIVDILV VWFVIYKVIM LIRGTKAVQL LKGIFIIIAV KLLSGFFGLQ
TVEWITDQML TWGFLAIIII FQPELRRALE TLGRGNIFTR YGSRIEREQH HLIESIEKST
QYMAKRRIGA LISVARDTGM DDYIETGIPL NAKISSQLLI NIFIPNTPLH DGAVIIKGNE
IASAASYLPL SDSPFLSKEL GTRHRAALGI SEVTDSITIV VSEETGGISL TKGGELFRDV
SEEELHKILL KELVTVTAKK PSIFSKWKGG KSE
