ID   DACB_MYCPN              Reviewed;         202 AA.
AC   P75528;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Diadenylate cyclase {ECO:0000255|HAMAP-Rule:MF_00838};
DE            Short=DAC {ECO:0000255|HAMAP-Rule:MF_00838};
DE            EC=2.7.7.85 {ECO:0000255|HAMAP-Rule:MF_00838};
DE   AltName: Full=Cyclic-di-AMP synthase {ECO:0000255|HAMAP-Rule:MF_00838};
DE            Short=c-di-AMP synthase {ECO:0000255|HAMAP-Rule:MF_00838};
DE   AltName: Full=Diadenylyl cyclase {ECO:0000303|PubMed:23812326};
GN   Name=dacB {ECO:0000255|HAMAP-Rule:MF_00838, ECO:0000303|PubMed:23812326};
GN   OrderedLocusNames=MPN_244; ORFNames=K04_orf202, MP588;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
RN   [2]
RP   GENE NAME.
RX   PubMed=23812326; DOI=10.1038/nrmicro3069;
RA   Corrigan R.M., Gruendling A.;
RT   "Cyclic di-AMP: another second messenger enters the fray.";
RL   Nat. Rev. Microbiol. 11:513-524(2013).
CC   -!- FUNCTION: Catalyzes the condensation of 2 ATP molecules into cyclic di-
CC       AMP (c-di-AMP), a second messenger used to regulate differing processes
CC       in different bacteria. {ECO:0000255|HAMAP-Rule:MF_00838}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 ATP = 3',3'-c-di-AMP + 2 diphosphate; Xref=Rhea:RHEA:35655,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:71500; EC=2.7.7.85;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00838};
CC   -!- SUBUNIT: Probably oligomerizes. {ECO:0000255|HAMAP-Rule:MF_00838}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_00838};
CC       Single-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_00838}.
CC   -!- SIMILARITY: Belongs to the adenylate cyclase family. DacB/CdaS
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_00838}.
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DR   EMBL; U00089; AAB96236.1; -; Genomic_DNA.
DR   PIR; S73914; S73914.
DR   RefSeq; NP_109932.1; NC_000912.1.
DR   RefSeq; WP_010874601.1; NC_000912.1.
DR   AlphaFoldDB; P75528; -.
DR   SMR; P75528; -.
DR   STRING; 272634.MPN_244; -.
DR   EnsemblBacteria; AAB96236; AAB96236; MPN_244.
DR   GeneID; 66609110; -.
DR   KEGG; mpn:MPN_244; -.
DR   PATRIC; fig|272634.6.peg.263; -.
DR   HOGENOM; CLU_038561_2_0_14; -.
DR   OMA; YAATFYK; -.
DR   BioCyc; MPNE272634:G1GJ3-387-MON; -.
DR   BRENDA; 2.7.7.85; 3534.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004016; F:adenylate cyclase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0106408; F:diadenylate cyclase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006171; P:cAMP biosynthetic process; IEA:InterPro.
DR   GO; GO:0019932; P:second-messenger-mediated signaling; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.1700.10; -; 1.
DR   HAMAP; MF_00838; DacB; 1.
DR   InterPro; IPR014046; C-di-AMP_synthase.
DR   InterPro; IPR034701; CdaA.
DR   InterPro; IPR034693; CdaS.
DR   InterPro; IPR036888; DNA_integrity_DisA_N_sf.
DR   InterPro; IPR003390; DNA_integrity_scan_DisA_N.
DR   Pfam; PF02457; DAC; 1.
DR   PIRSF; PIRSF004793; UCP004793; 1.
DR   SUPFAM; SSF143597; SSF143597; 1.
DR   TIGRFAMs; TIGR00159; TIGR00159; 1.
DR   PROSITE; PS51794; DAC; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW   Nucleotidyltransferase; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..202
FT                   /note="Diadenylate cyclase"
FT                   /id="PRO_0000210418"
FT   TRANSMEM        6..26
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00838"
FT   DOMAIN          29..185
FT                   /note="DAC"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00838"
SQ   SEQUENCE   202 AA;  22733 MW;  F2E3F12431ADCE18 CRC64;
     MMTVEVFSVI ILVLLFLILA LTLLFVLLNK RTRSFVIRTF TGLFRSKHTT SQKNFYDNLT
     STLLRLSTDK IGAIIAIENQ DSLESYVNIG YRVTSDFSPE LLVTIFYNKQ SPLHDGAVIV
     RDYQIVSVSS YFPMTRQLID VSYGSRHRSA LGLTEKCDAI VFIVSETTGK ISVAVRGVIK
     TLSSNSDRLQ DQIIHYLTVK PG