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DACC_ECOLI
ID   DACC_ECOLI              Reviewed;         400 AA.
AC   P08506; P77287;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 190.
DE   RecName: Full=D-alanyl-D-alanine carboxypeptidase DacC;
DE            Short=DD-carboxypeptidase;
DE            Short=DD-peptidase;
DE            EC=3.4.16.4;
DE   AltName: Full=Penicillin-binding protein 6;
DE            Short=PBP-6;
DE   Flags: Precursor;
GN   Name=dacC; OrderedLocusNames=b0839, JW0823;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=3279397; DOI=10.1093/nar/16.4.1617;
RA   Broome-Smith J.K., Ioannidis I., Edelman A., Spratt B.G.;
RT   "Nucleotide sequences of the penicillin-binding protein 5 and 6 genes of
RT   Escherichia coli.";
RL   Nucleic Acids Res. 16:1617-1617(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [5]
RP   PROTEIN SEQUENCE OF 28-52.
RX   PubMed=7042389; DOI=10.1016/0014-5793(82)80840-5;
RA   Waxman D.J., Amanuma H., Strominger J.L.;
RT   "Amino acid sequence homologies between Escherichia coli penicillin-binding
RT   protein 5 and class A beta-lactamases.";
RL   FEBS Lett. 139:159-163(1982).
RN   [6]
RP   SUBCELLULAR LOCATION, AND DOMAIN.
RX   PubMed=3330754; DOI=10.1111/j.1365-2958.1987.tb00522.x;
RA   Jackson M.E., Pratt J.M.;
RT   "An 18 amino acid amphiphilic helix forms the membrane-anchoring domain of
RT   the Escherichia coli penicillin-binding protein 5.";
RL   Mol. Microbiol. 1:23-28(1987).
CC   -!- FUNCTION: Removes C-terminal D-alanyl residues from sugar-peptide cell
CC       wall precursors.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also
CC         transpeptidation of peptidyl-alanyl moieties that are N-acyl
CC         substituents of D-alanine.; EC=3.4.16.4;
CC   -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:3330754};
CC       Peripheral membrane protein {ECO:0000269|PubMed:3330754}; Periplasmic
CC       side {ECO:0000269|PubMed:3330754}. Note=N-terminus lies in the
CC       periplasmic space, targeted there by the C-terminal amphiphilic helix
CC       (PMID:3330754).
CC   -!- DOMAIN: The C-terminus forms an amphiphilic helix that targets the
CC       protein to the periplasmic side of the inner cell membrane.
CC       {ECO:0000269|PubMed:3330754}.
CC   -!- SIMILARITY: Belongs to the peptidase S11 family. {ECO:0000305}.
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DR   EMBL; X06480; CAA29775.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73926.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35542.1; -; Genomic_DNA.
DR   PIR; G64821; G64821.
DR   RefSeq; NP_415360.1; NC_000913.3.
DR   RefSeq; WP_001300708.1; NZ_SSZK01000002.1.
DR   PDB; 3IT9; X-ray; 2.10 A; A/B/C/D=28-378.
DR   PDB; 3ITA; X-ray; 1.80 A; A/B/C/D=28-378.
DR   PDB; 3ITB; X-ray; 1.80 A; A/B/C/D=28-378.
DR   PDBsum; 3IT9; -.
DR   PDBsum; 3ITA; -.
DR   PDBsum; 3ITB; -.
DR   AlphaFoldDB; P08506; -.
DR   SMR; P08506; -.
DR   BioGRID; 4262825; 228.
DR   IntAct; P08506; 2.
DR   MINT; P08506; -.
DR   STRING; 511145.b0839; -.
DR   ChEMBL; CHEMBL2354204; -.
DR   DrugBank; DB01602; Bacampicillin.
DR   DrugBank; DB00578; Carbenicillin.
DR   DrugBank; DB09319; Carindacillin.
DR   DrugBank; DB00274; Cefmetazole.
DR   DrugBank; DB01329; Cefoperazone.
DR   DrugBank; DB01331; Cefoxitin.
DR   DrugBank; DB00430; Cefpiramide.
DR   DrugBank; DB01332; Ceftizoxime.
DR   DrugBank; DB09050; Ceftolozane.
DR   DrugBank; DB01000; Cyclacillin.
DR   DrugBank; DB00303; Ertapenem.
DR   DrugCentral; P08506; -.
DR   MEROPS; S11.003; -.
DR   jPOST; P08506; -.
DR   PaxDb; P08506; -.
DR   PRIDE; P08506; -.
DR   EnsemblBacteria; AAC73926; AAC73926; b0839.
DR   EnsemblBacteria; BAA35542; BAA35542; BAA35542.
DR   GeneID; 945455; -.
DR   KEGG; ecj:JW0823; -.
DR   KEGG; eco:b0839; -.
DR   PATRIC; fig|1411691.4.peg.1439; -.
DR   EchoBASE; EB0199; -.
DR   eggNOG; COG1686; Bacteria.
DR   HOGENOM; CLU_027070_8_1_6; -.
DR   InParanoid; P08506; -.
DR   OMA; TNEAKYC; -.
DR   PhylomeDB; P08506; -.
DR   BioCyc; EcoCyc:EG10203-MON; -.
DR   BioCyc; MetaCyc:EG10203-MON; -.
DR   UniPathway; UPA00219; -.
DR   EvolutionaryTrace; P08506; -.
DR   PRO; PR:P08506; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IDA:EcoliWiki.
DR   GO; GO:0030288; C:outer membrane-bounded periplasmic space; IDA:EcoCyc.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   GO; GO:0004180; F:carboxypeptidase activity; IDA:EcoCyc.
DR   GO; GO:0008658; F:penicillin binding; IDA:EcoCyc.
DR   GO; GO:0009002; F:serine-type D-Ala-D-Ala carboxypeptidase activity; ISM:EcoCyc.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   GO; GO:0009252; P:peptidoglycan biosynthetic process; IDA:EcoCyc.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
DR   GO; GO:0009410; P:response to xenobiotic stimulus; IMP:EcoCyc.
DR   Gene3D; 2.60.410.10; -; 1.
DR   Gene3D; 3.40.710.10; -; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR015956; Peniciliin-bd_prot_C_sf.
DR   InterPro; IPR018044; Peptidase_S11.
DR   InterPro; IPR012907; Peptidase_S11_C.
DR   InterPro; IPR037167; Peptidase_S11_C_sf.
DR   InterPro; IPR001967; Peptidase_S11_N.
DR   Pfam; PF07943; PBP5_C; 1.
DR   Pfam; PF00768; Peptidase_S11; 1.
DR   PRINTS; PR00725; DADACBPTASE1.
DR   SMART; SM00936; PBP5_C; 1.
DR   SUPFAM; SSF56601; SSF56601; 1.
DR   SUPFAM; SSF69189; SSF69189; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Carboxypeptidase; Cell inner membrane; Cell membrane;
KW   Cell shape; Cell wall biogenesis/degradation; Direct protein sequencing;
KW   Hydrolase; Membrane; Peptidoglycan synthesis; Protease; Reference proteome;
KW   Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000269|PubMed:7042389"
FT   CHAIN           28..400
FT                   /note="D-alanyl-D-alanine carboxypeptidase DacC"
FT                   /id="PRO_0000027233"
FT   REGION          383..400
FT                   /note="Required for inner membrane binding"
FT   ACT_SITE        66
FT                   /note="Acyl-ester intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        69
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        132
FT                   /evidence="ECO:0000250"
FT   BINDING         235
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        231
FT                   /note="V -> E (in Ref. 1; CAA29775)"
FT                   /evidence="ECO:0000305"
FT   STRAND          39..46
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   TURN            47..49
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          52..57
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           65..67
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           68..81
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          90..92
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           95..97
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   TURN            99..101
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           103..105
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          118..120
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           121..129
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           134..145
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           148..161
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          171..173
FT                   /evidence="ECO:0007829|PDB:3ITB"
FT   HELIX           184..197
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           199..202
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           203..206
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          209..212
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          215..218
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           222..225
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          230..239
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   TURN            240..242
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          243..252
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          255..266
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           267..284
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          285..288
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          297..313
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           315..317
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          320..323
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   HELIX           327..329
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          330..343
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          350..358
FT                   /evidence="ECO:0007829|PDB:3ITA"
FT   STRAND          361..370
FT                   /evidence="ECO:0007829|PDB:3ITA"
SQ   SEQUENCE   400 AA;  43609 MW;  4D7F3BDCEA64C775 CRC64;
     MTQYSSLLRG LAAGSAFLFL FAPTAFAAEQ TVEAPSVDAR AWILMDYASG KVLAEGNADE
     KLDPASLTKI MTSYVVGQAL KADKIKLTDM VTVGKDAWAT GNPALRGSSV MFLKPGDQVS
     VADLNKGVII QSGNDACIAL ADYVAGSQES FIGLMNGYAK KLGLTNTTFQ TVHGLDAPGQ
     FSTARDMALL GKALIHDVPE EYAIHKEKEF TFNKIRQPNR NRLLWSSNLN VDGMKTGTTA
     GAGYNLVASA TQGDMRLISV VLGAKTDRIR FNESEKLLTW GFRFFETVTP IKPDATFVTQ
     RVWFGDKSEV NLGAGEAGSV TIPRGQLKNL KASYTLTEPQ LTAPLKKGQV VGTIDFQLNG
     KSIEQRPLIV MENVEEGGFF GRVWDFVMMK FHQWFGSWFS
 
 
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