DACH2_MOUSE
ID DACH2_MOUSE Reviewed; 634 AA.
AC Q925Q8; Q8BMA0;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Dachshund homolog 2;
DE Short=Dach2;
GN Name=Dach2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Swiss Webster / NIH;
RX PubMed=11287190; DOI=10.1016/s0925-4773(01)00307-0;
RA Davis R.J., Shen W., Sandler Y.I., Heanue T.A., Mardon G.;
RT "Characterization of mouse Dach2, a homologue of Drosophila dachshund.";
RL Mech. Dev. 102:169-179(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, AND INTERACTION WITH SIX6.
RX PubMed=12130660; DOI=10.1126/science.1073263;
RA Li X., Perissi V., Liu F., Rose D.W., Rosenfeld M.G.;
RT "Tissue-specific regulation of retinal and pituitary precursor cell
RT proliferation.";
RL Science 297:1180-1183(2002).
RN [5]
RP FUNCTION.
RX PubMed=12112464; DOI=10.1002/dvdy.10107;
RA Kardon G., Heanue T.A., Tabin C.J.;
RT "Pax3 and Dach2 positive regulation in the developing somite.";
RL Dev. Dyn. 224:350-355(2002).
CC -!- FUNCTION: Transcription factor that is involved in regulation of
CC organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act
CC as a corepressor of SIX6 in regulating proliferation by directly
CC repressing cyclin-dependent kinase inhibitors, including the p27Kip1
CC promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal
CC retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and
CC HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation
CC of myogenesis. In the developing somite, expression of DACH2 and PAX3
CC is regulated by the overlying ectoderm, and DACH2 and PAX3 positively
CC regulate each other's expression. Probably binds to DNA via its
CC DACHbox-N domain. {ECO:0000269|PubMed:12112464,
CC ECO:0000269|PubMed:12130660}.
CC -!- SUBUNIT: Interacts with SIX6. Interacts with EYA2 (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q925Q8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q925Q8-2; Sequence=VSP_009493, VSP_009494;
CC Name=3;
CC IsoId=Q925Q8-3; Sequence=VSP_009495, VSP_009496, VSP_009497;
CC -!- TISSUE SPECIFICITY: Expressed in embryo, and at lower levels in the
CC newborn. {ECO:0000269|PubMed:11287190}.
CC -!- DEVELOPMENTAL STAGE: From 8.5 to 14.5 dpc detected in nervous tissue.
CC In the brain detected at 8.5 dpc within the prospective hindbrain, but
CC not within the developing forebrain or midbrain. At 9.5 dpc expressed
CC within the ventral prosencephalon, hindbrain and forebrain. Expression
CC within the forebrain neuroectoderm flanked the optic vesicle with
CC rostral and caudal restrictions. In addition, dorsal and ventral
CC expression domains were observed within the hindbrain. At 10.5 to 12.5
CC dpc detected in the dorsal mesencephalon, in addition to the
CC telencephalon and hindbrain. At 14.5 dpc visible in the olfactory
CC bulbs. Detected from 9.5 to 12.5 dpc in the dorsal neural tube with the
CC highest expression near the hindbrain. At 9.5 and 10.5 dpc detected in
CC cells located in the dorsal and ventral neural tube and dorsal root
CC ganglia. Detected during the development of the optic and the auditory
CC systems. At 10.5 dpc, a small ring of ocular staining was observed
CC suggesting expression in the lens pit. At 10.5 dpc expressed in the
CC developing lens epithelium and in the mesenchyme surrounding the
CC retina. However, expression in the lens placode ectoderm was not
CC detected, suggesting that DACH2 is activated after lens vesicle
CC formation. Low levels of expression could also be detected in the
CC peripheral neuroretina at 10.5 and 12.5 dpc. Detected in the otic pit
CC at 9.5 dpc and in the otic endolymphatic duct at 10.5, 11.5 and 12.5
CC dpc. From 10.5 to 14.5 dpc detected in the developing fore and hind
CC limbs. At 10.5 and 11.5 dpc observed in the anterior and posterior
CC margins and presumptive hand plate of the limb bud. At 9.5 and 10.5 dpc
CC expressed in the limb. At 12.5 dpc is detected in the hand plate with
CC strong expression at the margins of the limb plate. At 14.5 dpc
CC detected in the hand plate and lateral edges of the digits. At 8.5 dpc
CC expression was not detected in the developing somites. In contrast,
CC from 9.5 to 12.5 dpc, expression is detected in a repeated pattern
CC located lateral to the neural tube and in the interlimb bud region
CC suggesting expression in somite derivatives. At 9.5 dpc detected in the
CC forelimb dermamyotome. Also located along the lateral portion of the
CC trunk and within a dorsal domain within the limb bud. At 10.5 dpc
CC expressed in lateral and limb mesoderm. From 9.5 to 10.5 dpc detected
CC in head mesenchyme and the branchial arches. At 9.5 and 10 dpc.
CC expressed in the head mesoderm associated with the developing eye. At
CC 10.5 dpc this pattern appears as a ring of expression surrounding the
CC eye. At 11.5 dpc expression is still detectable in the branchial arches
CC with strong expression at the cranial sinus. At 14.5 dpc mammary gland
CC primordia. Detected in the nasal openings and vibrissae.
CC {ECO:0000269|PubMed:11287190}.
CC -!- DOMAIN: The DACHbox-N domain forms a structure containing a DNA binding
CC motif similar to that of the forkhead/winged helix domain.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DACH/dachshund family. {ECO:0000305}.
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DR EMBL; AF257217; AAK39983.1; -; mRNA.
DR EMBL; AK033050; BAC28138.1; -; mRNA.
DR EMBL; BC059233; AAH59233.1; -; mRNA.
DR CCDS; CCDS30363.1; -. [Q925Q8-1]
DR CCDS; CCDS53175.1; -. [Q925Q8-2]
DR CCDS; CCDS81163.1; -. [Q925Q8-3]
DR RefSeq; NP_001136042.1; NM_001142570.1. [Q925Q8-2]
DR RefSeq; NP_001276661.1; NM_001289732.1. [Q925Q8-3]
DR RefSeq; NP_001276662.1; NM_001289733.1.
DR RefSeq; NP_001276663.1; NM_001289734.1.
DR RefSeq; NP_291083.1; NM_033605.2. [Q925Q8-1]
DR RefSeq; XP_011246018.1; XM_011247716.1. [Q925Q8-3]
DR AlphaFoldDB; Q925Q8; -.
DR SMR; Q925Q8; -.
DR IntAct; Q925Q8; 1.
DR STRING; 10090.ENSMUSP00000064393; -.
DR iPTMnet; Q925Q8; -.
DR PhosphoSitePlus; Q925Q8; -.
DR MaxQB; Q925Q8; -.
DR PaxDb; Q925Q8; -.
DR PeptideAtlas; Q925Q8; -.
DR PRIDE; Q925Q8; -.
DR ProteomicsDB; 279271; -. [Q925Q8-1]
DR ProteomicsDB; 279272; -. [Q925Q8-2]
DR ProteomicsDB; 279273; -. [Q925Q8-3]
DR Antibodypedia; 417; 172 antibodies from 30 providers.
DR DNASU; 93837; -.
DR Ensembl; ENSMUST00000067219; ENSMUSP00000064393; ENSMUSG00000025592. [Q925Q8-1]
DR Ensembl; ENSMUST00000113380; ENSMUSP00000109007; ENSMUSG00000025592. [Q925Q8-3]
DR Ensembl; ENSMUST00000113382; ENSMUSP00000109009; ENSMUSG00000025592. [Q925Q8-2]
DR GeneID; 93837; -.
DR KEGG; mmu:93837; -.
DR UCSC; uc009udp.1; mouse. [Q925Q8-3]
DR UCSC; uc009udr.2; mouse. [Q925Q8-2]
DR UCSC; uc009uds.1; mouse. [Q925Q8-1]
DR CTD; 117154; -.
DR MGI; MGI:1890446; Dach2.
DR VEuPathDB; HostDB:ENSMUSG00000025592; -.
DR eggNOG; KOG3915; Eukaryota.
DR GeneTree; ENSGT00390000001134; -.
DR HOGENOM; CLU_027923_0_0_1; -.
DR InParanoid; Q925Q8; -.
DR OMA; PSQMDHQ; -.
DR OrthoDB; 429758at2759; -.
DR PhylomeDB; Q925Q8; -.
DR TreeFam; TF316697; -.
DR BioGRID-ORCS; 93837; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Dach2; mouse.
DR PRO; PR:Q925Q8; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q925Q8; protein.
DR Bgee; ENSMUSG00000025592; Expressed in vas deferens and 141 other tissues.
DR ExpressionAtlas; Q925Q8; baseline and differential.
DR Genevisible; Q925Q8; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0046545; P:development of primary female sexual characteristics; IGI:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.10.260.20; -; 1.
DR InterPro; IPR009061; DNA-bd_dom_put_sf.
DR InterPro; IPR003380; SKI/SNO/DAC.
DR InterPro; IPR037000; Ski_DNA-bd_sf.
DR Pfam; PF02437; Ski_Sno; 1.
DR SUPFAM; SSF46955; SSF46955; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Developmental protein; DNA-binding;
KW Nucleus; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..634
FT /note="Dachshund homolog 2"
FT /id="PRO_0000095600"
FT REGION 76..162
FT /note="DACHbox-N"
FT REGION 171..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 244..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 378..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 488..568
FT /note="DACHbox-C"
FT COILED 494..588
FT /evidence="ECO:0000255"
FT COMPBIAS 244..276
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 390..415
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..174
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_009495"
FT VAR_SEQ 170..182
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_009493"
FT VAR_SEQ 449
FT /note="E -> DIQLSQHHLLNTFLHWIELAPLSKKSLHHQK (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_009496"
FT VAR_SEQ 498
FT /note="Q -> QVNNISINKIN (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_009497"
FT VAR_SEQ 619..634
FT /note="GGNYYCLAMAQQLCSA -> A (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_009494"
FT CONFLICT 507
FT /note="N -> D (in Ref. 2; BAC28138)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 634 AA; 68588 MW; 838A491FFF20C410 CRC64;
MAVSAPPVIS ATSSSAGVPG GLFRAEPLYS SPGEPPRLTP NMINSFMANN HNGSVLGGGI
GGGSGGSSNT NTNECRMVDM HGVKVASFLM DGQELICLPQ VFDLFLKHLV GGLHTVYTKL
KRLDISPVVC TVEQVRILRG LGAIQPGVNR CKLITRKDFE TLFTDCTNAR RKRQMTRKQA
VNSSRPGRPP KRSLGVLQDN ARLLPHAVPG LLSPGLITPT GITAAAMAEA MKLQKMKLMA
MNTLQGNGSQ NGTESEPDDL NSTTGGSESS WDKDKIQSPL AASGPQHGIA HAALAGQPGL
GGAPTLNPLQ QNHLLSNRLD LPFMMMPHPL LPVSLPPASV AMAMNQMNHL NTIANMAAAA
QIHSPLSRAG ASVIKERIPE SPSPAPSLEE SHRPGSQTSS HPSSSVSSSP SQMDHHSERM
VMMPNNREEL IVDQDNGQSI KKFQRDNKEE VPAQIPVMKS PLDKIQLAPG QALHPGFPGP
FIFADSLSSV ETLLTNIQGL LKVALDNARI QEKQIQQEKK ELRIELFRER EIRENLERQL
AVELQSRSTM QKRLKKEKKA KRKLQEALEF ESKRREQVEQ ALKQATSGDS GLRMLKDSGI
PDIEIENSGT PHDSAAMQGG NYYCLAMAQQ LCSA
