ID   DAD1_SOLLC              Reviewed;         116 AA.
AC   Q9SMC4;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1;
DE            Short=Oligosaccharyl transferase subunit DAD1;
DE   AltName: Full=Defender against cell death 1;
DE            Short=DAD-1;
GN   Name=DAD1;
OS   Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC   Solanum subgen. Lycopersicon.
OX   NCBI_TaxID=4081;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Prisca;
RA   Hoeberichts F.A., Woltering E.J.;
RT   "Cloning and analysis of a defender against apoptotic cell death (DAD1)
RT   homologue from tomato.";
RL   J. Plant Physiol. 158:125-128(2001).
CC   -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC       catalyzes the initial transfer of a defined glycan
CC       (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC       pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC       consensus motif in nascent polypeptide chains, the first step in
CC       protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC       the complex associates with the Sec61 complex at the channel-forming
CC       translocon complex that mediates protein translocation across the
CC       endoplasmic reticulum (ER). All subunits are required for a maximal
CC       enzyme activity. {ECO:0000250|UniProtKB:P46964}.
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC   -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC       {ECO:0000250|UniProtKB:P46964}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DAD/OST2 family. {ECO:0000305}.
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DR   EMBL; AJ250003; CAB61887.1; -; mRNA.
DR   RefSeq; NP_001233817.1; NM_001246888.1.
DR   AlphaFoldDB; Q9SMC4; -.
DR   SMR; Q9SMC4; -.
DR   STRING; 4081.Solyc08g076460.2.1; -.
DR   PaxDb; Q9SMC4; -.
DR   EnsemblPlants; Solyc08g076460.3.1; Solyc08g076460.3.1; Solyc08g076460.3.
DR   GeneID; 543753; -.
DR   Gramene; Solyc08g076460.3.1; Solyc08g076460.3.1; Solyc08g076460.3.
DR   KEGG; sly:543753; -.
DR   eggNOG; KOG1746; Eukaryota.
DR   HOGENOM; CLU_111220_2_0_1; -.
DR   InParanoid; Q9SMC4; -.
DR   OMA; FIFAHII; -.
DR   OrthoDB; 1586516at2759; -.
DR   PhylomeDB; Q9SMC4; -.
DR   UniPathway; UPA00378; -.
DR   Proteomes; UP000004994; Chromosome 8.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0008250; C:oligosaccharyltransferase complex; IBA:GO_Central.
DR   GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR   InterPro; IPR003038; DAD/Ost2.
DR   PANTHER; PTHR10705; PTHR10705; 1.
DR   Pfam; PF02109; DAD; 1.
DR   PIRSF; PIRSF005588; DAD; 1.
PE   3: Inferred from homology;
KW   Apoptosis; Endoplasmic reticulum; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..116
FT                   /note="Dolichyl-diphosphooligosaccharide--protein
FT                   glycosyltransferase subunit DAD1"
FT                   /id="PRO_0000124025"
FT   TOPO_DOM        1..32
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        33..53
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        54..56
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        78..95
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        96..116
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   116 AA;  12671 MW;  F4EABA533E8BDBA6 CRC64;
     MAKSSATKDA QALFHSLRSA YAATPTNLKI IDLYVIFAIS TALIQVVYMA IVGSFPFNSF
     LSGVLSCIGT AVLAVCLRIQ VNKENKEFKD LPPERAFADF VLCNLVLHLV IMNFLG