DAD2_ARATH
ID DAD2_ARATH Reviewed; 115 AA.
AC O22622; Q94A89; Q9SLH6;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD2;
DE Short=Oligosaccharyl transferase subunit DAD2;
DE AltName: Full=Defender against cell death 2;
DE Short=AtDAD2;
DE Short=DAD-2;
GN Name=DAD2; OrderedLocusNames=At2g35520; ORFNames=T32F12.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Danon A., Gallois P.;
RT "AtDAD2: a new gene from the DAD1 family in Arabidopsis.";
RL Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Sugiura T., Naito K., Asahi T., Suzuki H.;
RT "Differential regulation of two types of the defender against apoptotic
RT cell death 1 (dad1) genes in senescing cotyledons and petals of Arabidopsis
RT thaliana.";
RL Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Subunit of the oligosaccharyl transferase (OST) complex that
CC catalyzes the initial transfer of a defined glycan
CC (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-
CC pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr
CC consensus motif in nascent polypeptide chains, the first step in
CC protein N-glycosylation. N-glycosylation occurs cotranslationally and
CC the complex associates with the Sec61 complex at the channel-forming
CC translocon complex that mediates protein translocation across the
CC endoplasmic reticulum (ER). All subunits are required for a maximal
CC enzyme activity. {ECO:0000250|UniProtKB:P46964}.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC -!- SUBUNIT: Component of the oligosaccharyltransferase (OST) complex.
CC {ECO:0000250|UniProtKB:P46964}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O22622-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O22622-2; Sequence=VSP_008913, VSP_008914;
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the DAD/OST2 family. {ECO:0000305}.
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DR EMBL; AF030172; AAB86478.1; -; Genomic_DNA.
DR EMBL; Y17609; CAC80055.1; -; Genomic_DNA.
DR EMBL; AC005314; AAC36169.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC09116.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM62783.1; -; Genomic_DNA.
DR EMBL; AK119013; BAC43589.1; -; mRNA.
DR EMBL; AY049271; AAK83613.1; -; mRNA.
DR EMBL; AY087959; AAM65506.1; -; mRNA.
DR PIR; F84769; F84769.
DR RefSeq; NP_001318358.1; NM_001336559.1. [O22622-1]
DR RefSeq; NP_565807.1; NM_129104.4. [O22622-1]
DR AlphaFoldDB; O22622; -.
DR SMR; O22622; -.
DR BioGRID; 3463; 63.
DR IntAct; O22622; 63.
DR STRING; 3702.AT2G35520.2; -.
DR PaxDb; O22622; -.
DR ProteomicsDB; 224703; -. [O22622-1]
DR EnsemblPlants; AT2G35520.1; AT2G35520.1; AT2G35520. [O22622-1]
DR EnsemblPlants; AT2G35520.3; AT2G35520.3; AT2G35520. [O22622-1]
DR GeneID; 818117; -.
DR Gramene; AT2G35520.1; AT2G35520.1; AT2G35520. [O22622-1]
DR Gramene; AT2G35520.3; AT2G35520.3; AT2G35520. [O22622-1]
DR KEGG; ath:AT2G35520; -.
DR Araport; AT2G35520; -.
DR eggNOG; KOG1746; Eukaryota.
DR InParanoid; O22622; -.
DR OMA; AFLEFCF; -.
DR PhylomeDB; O22622; -.
DR UniPathway; UPA00378; -.
DR PRO; PR:O22622; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O22622; baseline and differential.
DR Genevisible; O22622; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008250; C:oligosaccharyltransferase complex; IBA:GO_Central.
DR GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR InterPro; IPR003038; DAD/Ost2.
DR PANTHER; PTHR10705; PTHR10705; 1.
DR Pfam; PF02109; DAD; 1.
DR PIRSF; PIRSF005588; DAD; 1.
PE 3: Inferred from homology;
KW Alternative splicing; Apoptosis; Endoplasmic reticulum; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..115
FT /note="Dolichyl-diphosphooligosaccharide--protein
FT glycosyltransferase subunit DAD2"
FT /id="PRO_0000124020"
FT TOPO_DOM 1..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..55
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..94
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 29..53
FT /note="IIDLYVCFAVFTALIQVAYMALVGS -> VHHYHFVLIFYESSLKLKNPLNV
FT LV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_008913"
FT VAR_SEQ 54..115
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14593172"
FT /id="VSP_008914"
SQ SEQUENCE 115 AA; 12647 MW; A9B76DDF6ABA5F62 CRC64;
MVKSTSKDAQ DLFHSLHSAY TATPTNLKII DLYVCFAVFT ALIQVAYMAL VGSFPFNSFL
SGVLSCIGTA VLAVCLRIQV NKENKEFKDL APERAFADFV LCNLVLHLVI INFLG
