DAD2_SCHPO
ID DAD2_SCHPO Reviewed; 94 AA.
AC Q9UTG8;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=DASH complex subunit dad2;
DE AltName: Full=High osmolarity sensitivity protein 2;
DE AltName: Full=Outer kinetochore protein dad2;
GN Name=dad2; Synonyms=hos2; ORFNames=SPAC1805.07c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION.
RX PubMed=11193425; DOI=10.1271/bbb.64.2493;
RA Nakamichi N., Yamamoto E., Yamada H., Aiba H., Mizuno T.;
RT "Identification and characterization of a novel gene, hos2+, the function
RT of which is necessary for growth under high osmotic stress in fission
RT yeast.";
RL Biosci. Biotechnol. Biochem. 64:2493-2496(2000).
RN [3]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
CC -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC subcomplex of the outer kinetochore that is essential for proper
CC chromosome segregation. The DASH complex mediates the formation and
CC maintenance of bipolar kinetochore-microtubule attachments by forming
CC closed rings around spindle microtubules and establishing interactions
CC with proteins from the central kinetochore. May be necessary for growth
CC under high osmotic stress. {ECO:0000269|PubMed:11193425,
CC ECO:0000269|PubMed:16079914}.
CC -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC microtubules. {ECO:0000269|PubMed:16079914}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16079914}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:16079914}. Chromosome,
CC centromere, kinetochore {ECO:0000269|PubMed:16079914}. Note=Associates
CC with the mitotic spindle and the kinetochore. Kinetochore association
CC occurs only during mitosis.
CC -!- SIMILARITY: Belongs to the DASH complex DAD2 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB55848.1; -; Genomic_DNA.
DR PIR; T37892; T37892.
DR RefSeq; NP_593918.1; NM_001019347.2.
DR AlphaFoldDB; Q9UTG8; -.
DR SMR; Q9UTG8; -.
DR BioGRID; 278881; 365.
DR STRING; 4896.SPAC1805.07c.1; -.
DR MaxQB; Q9UTG8; -.
DR PaxDb; Q9UTG8; -.
DR EnsemblFungi; SPAC1805.07c.1; SPAC1805.07c.1:pep; SPAC1805.07c.
DR GeneID; 2542418; -.
DR KEGG; spo:SPAC1805.07c; -.
DR PomBase; SPAC1805.07c; dad2.
DR VEuPathDB; FungiDB:SPAC1805.07c; -.
DR eggNOG; ENOG502SG7I; Eukaryota.
DR HOGENOM; CLU_138063_5_0_1; -.
DR InParanoid; Q9UTG8; -.
DR OMA; KQHEYAS; -.
DR PhylomeDB; Q9UTG8; -.
DR PRO; PR:Q9UTG8; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0042729; C:DASH complex; IDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:1990023; C:mitotic spindle midzone; IDA:PomBase.
DR GO; GO:0044732; C:mitotic spindle pole body; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0008608; P:attachment of spindle microtubules to kinetochore; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:1990758; P:mitotic sister chromatid biorientation; TAS:PomBase.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IC:PomBase.
DR InterPro; IPR013963; DASH_Dad2.
DR PANTHER; PTHR28036; PTHR28036; 1.
DR Pfam; PF08654; DASH_Dad2; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..94
FT /note="DASH complex subunit dad2"
FT /id="PRO_0000211596"
FT REGION 72..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 18..38
FT /evidence="ECO:0000255"
FT COMPBIAS 72..88
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 94 AA; 10319 MW; 064CFD495102560A CRC64;
MLQARIEEKQ KEYELICKLR DSSNDMVQQI ETLAAKLETL TDGSEAVATV LNNWPSIFES
IQIASQHSGA LVRIPPSTSN TNASATEQGD VEEV
