ID   DAD3_KLULA              Reviewed;          85 AA.
AC   Q6CKH5;
DT   21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=DASH complex subunit DAD3;
DE   AltName: Full=Outer kinetochore protein DAD3;
GN   Name=DAD3; OrderedLocusNames=KLLA0F10637g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. The DASH complex mediates the formation and
CC       maintenance of bipolar kinetochore-microtubule attachments by forming
CC       closed rings around spindle microtubules and establishing interactions
CC       with proteins from the central kinetochore (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC       microtubules. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton,
CC       spindle {ECO:0000250}. Chromosome, centromere, kinetochore
CC       {ECO:0000250}. Note=Associates with the mitotic spindle and the
CC       kinetochore. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DASH complex DAD3 family. {ECO:0000305}.
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DR   EMBL; CR382126; CAG98272.1; -; Genomic_DNA.
DR   RefSeq; XP_455564.1; XM_455564.1.
DR   AlphaFoldDB; Q6CKH5; -.
DR   SMR; Q6CKH5; -.
DR   STRING; 28985.XP_455564.1; -.
DR   PRIDE; Q6CKH5; -.
DR   EnsemblFungi; CAG98272; CAG98272; KLLA0_F10637g.
DR   GeneID; 2895731; -.
DR   KEGG; kla:KLLA0_F10637g; -.
DR   eggNOG; ENOG502S7SV; Eukaryota.
DR   HOGENOM; CLU_118180_2_1_1; -.
DR   InParanoid; Q6CKH5; -.
DR   OMA; LNNTHED; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0042729; C:DASH complex; IEA:EnsemblFungi.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0072686; C:mitotic spindle; IEA:InterPro.
DR   GO; GO:0051010; F:microtubule plus-end binding; IEA:EnsemblFungi.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:1990758; P:mitotic sister chromatid biorientation; IEA:EnsemblFungi.
DR   GO; GO:0051987; P:positive regulation of attachment of spindle microtubules to kinetochore; IEA:EnsemblFungi.
DR   GO; GO:0031116; P:positive regulation of microtubule polymerization; IEA:EnsemblFungi.
DR   InterPro; IPR013965; DASH_Dad3.
DR   PANTHER; PTHR28017; PTHR28017; 1.
DR   Pfam; PF08656; DASH_Dad3; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..85
FT                   /note="DASH complex subunit DAD3"
FT                   /id="PRO_0000175949"
FT   COILED          13..41
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   85 AA;  9816 MW;  009B949B42B59979 CRC64;
     MSSELTPLQR SVLDKYRFLA ESLRELSETL SDLNNTHEDS KPEAILQEMR EIEVKISLVS
     TLLKGSVYSL VIQRRMDHQQ KPSDL