ID   DAD4_SCHPO              Reviewed;          72 AA.
AC   Q50HP4;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 2.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=DASH complex subunit dad4;
DE   AltName: Full=Outer kinetochore protein dad4;
GN   Name=dad4; ORFNames=SPBC3B9.22c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   FUNCTION, AND SUBUNIT.
RX   PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA   Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT   "Molecular analysis of kinetochore architecture in fission yeast.";
RL   EMBO J. 24:2919-2930(2005).
CC   -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC       subcomplex of the outer kinetochore that is essential for proper
CC       chromosome segregation. The DASH complex mediates the formation and
CC       maintenance of bipolar kinetochore-microtubule attachments by forming
CC       closed rings around spindle microtubules and establishing interactions
CC       with proteins from the central kinetochore. May be necessary for growth
CC       under high osmotic stress. {ECO:0000269|PubMed:16079914}.
CC   -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC       microtubules. {ECO:0000269|PubMed:16079914}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton,
CC       spindle {ECO:0000250}. Chromosome, centromere, kinetochore
CC       {ECO:0000250}. Note=Associates with the mitotic spindle and the
CC       kinetochore. Kinetochore association occurs only during mitosis (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DASH complex DAD4 family. {ECO:0000305}.
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DR   EMBL; CU329671; CAI94506.2; -; Genomic_DNA.
DR   RefSeq; XP_001713149.1; XM_001713097.2.
DR   AlphaFoldDB; Q50HP4; -.
DR   SMR; Q50HP4; -.
DR   BioGRID; 857966; 126.
DR   STRING; 4896.SPBC3B9.22c.1; -.
DR   MaxQB; Q50HP4; -.
DR   PaxDb; Q50HP4; -.
DR   EnsemblFungi; SPBC3B9.22c.1; SPBC3B9.22c.1:pep; SPBC3B9.22c.
DR   PomBase; SPBC3B9.22c; dad4.
DR   VEuPathDB; FungiDB:SPBC3B9.22c; -.
DR   eggNOG; ENOG502S890; Eukaryota.
DR   HOGENOM; CLU_177920_1_1_1; -.
DR   InParanoid; Q50HP4; -.
DR   OMA; SQMWANY; -.
DR   PhylomeDB; Q50HP4; -.
DR   PRO; PR:Q50HP4; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0042729; C:DASH complex; IDA:PomBase.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0072686; C:mitotic spindle; IEA:InterPro.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:1990758; P:mitotic sister chromatid biorientation; TAS:PomBase.
DR   GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IC:PomBase.
DR   InterPro; IPR013959; DASH_Dad4.
DR   PANTHER; PTHR28222; PTHR28222; 1.
DR   Pfam; PF08650; DASH_Dad4; 1.
PE   1: Evidence at protein level;
KW   Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW   Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..72
FT                   /note="DASH complex subunit dad4"
FT                   /id="PRO_0000176051"
FT   COILED          15..35
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   72 AA;  8351 MW;  F1518CABB8D2FCA9 CRC64;
     MNNPMEEQQS ALLGRIISNV EKLNESITRL NHSLQLINMS NMNVELASQM WANYARNVKF
     HLEETHTLKD PI