DAF14_CAEEL
ID DAF14_CAEEL Reviewed; 331 AA.
AC F5GUE5; G5EEP2;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 81.
DE RecName: Full=Smad-related protein daf-14 {ECO:0000305};
DE AltName: Full=Abnormal dauer formation protein 14 {ECO:0000312|WormBase:F01G10.8b};
GN Name=daf-14 {ECO:0000312|WormBase:F01G10.8b};
GN ORFNames=F01G10.8 {ECO:0000312|WormBase:F01G10.8b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAF03892.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, DEVELOPMENTAL STAGE, AND
RP MUTAGENESIS OF 236-GLN--SER-331; GLY-286 AND 302-TRP--SER-331.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAF03892.1};
RX PubMed=10625546; DOI=10.1006/dbio.1999.9545;
RA Inoue T., Thomas J.H.;
RT "Targets of TGF-beta signaling in Caenorhabditis elegans dauer formation.";
RL Dev. Biol. 217:192-204(2000).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, AND INTERACTION WITH DAF-8 AND DAF-3.
RX PubMed=20081192; DOI=10.1242/dev.043752;
RA Park D., Estevez A., Riddle D.L.;
RT "Antagonistic Smad transcription factors control the dauer/non-dauer switch
RT in C. elegans.";
RL Development 137:477-485(2010).
CC -!- FUNCTION: Probably an atypical receptor-regulated SMAD (R-SMAD) that is
CC an intracellular signal transducer and transcriptional modulator
CC activated by TGF-beta-like daf-7 signaling (PubMed:10625546). Plays a
CC role in TGF-beta-like daf-7 signaling in regulating entry into a
CC developmentally arrested larval state known as dauer, in response to
CC harsh environmental conditions; partially redundant with R-SMAD daf-8
CC (PubMed:10625546, PubMed:20081192). {ECO:0000269|PubMed:10625546,
CC ECO:0000269|PubMed:20081192}.
CC -!- SUBUNIT: Interacts with R-SMAD daf-8 and co-SMAD daf-3
CC (PubMed:20081192). Interacts with daf-3 in a daf-8 dependent manner
CC (PubMed:20081192). {ECO:0000269|PubMed:20081192}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b {ECO:0000312|WormBase:F01G10.8b};
CC IsoId=F5GUE5-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:F01G10.8a};
CC IsoId=F5GUE5-2; Sequence=VSP_060999;
CC -!- DEVELOPMENTAL STAGE: Widely expressed in all stages from the embryo to
CC the adult in a complex and dynamic pattern (PubMed:10625546). In L1 and
CC L2 stage larvae, expressed in intestinal cells, lateral hypodermal
CC cells, the anal sphincter muscle, phasmid sheath cells, neurons in the
CC lateral ganglia, and the excretory duct cell (PubMed:10625546).
CC {ECO:0000269|PubMed:10625546}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF190910; AAF03892.1; -; mRNA.
DR EMBL; BX284604; CAB02890.3; -; Genomic_DNA.
DR EMBL; BX284604; CCA65544.1; -; Genomic_DNA.
DR PIR; T20474; T20474.
DR RefSeq; NP_001255475.1; NM_001268546.1. [F5GUE5-1]
DR RefSeq; NP_001255476.1; NM_001268547.1. [F5GUE5-2]
DR AlphaFoldDB; F5GUE5; -.
DR SMR; F5GUE5; -.
DR STRING; 6239.F01G10.8b; -.
DR PaxDb; F5GUE5; -.
DR EnsemblMetazoa; F01G10.8a.1; F01G10.8a.1; WBGene00000910. [F5GUE5-2]
DR EnsemblMetazoa; F01G10.8b.1; F01G10.8b.1; WBGene00000910. [F5GUE5-1]
DR GeneID; 177908; -.
DR KEGG; cel:CELE_F01G10.8; -.
DR CTD; 177908; -.
DR WormBase; F01G10.8a; CE29090; WBGene00000910; daf-14.
DR WormBase; F01G10.8b; CE45907; WBGene00000910; daf-14.
DR eggNOG; KOG3701; Eukaryota.
DR GeneTree; ENSGT00940000171601; -.
DR HOGENOM; CLU_052086_0_0_1; -.
DR InParanoid; F5GUE5; -.
DR OMA; INDWLED; -.
DR PhylomeDB; F5GUE5; -.
DR Reactome; R-CEL-1181150; Signaling by NODAL.
DR Reactome; R-CEL-1502540; Signaling by Activin.
DR Reactome; R-CEL-2173788; Downregulation of TGF-beta receptor signaling.
DR Reactome; R-CEL-2173789; TGF-beta receptor signaling activates SMADs.
DR Reactome; R-CEL-2173795; Downregulation of SMAD2/3:SMAD4 transcriptional activity.
DR Reactome; R-CEL-2173796; SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription.
DR Reactome; R-CEL-5689880; Ub-specific processing proteases.
DR Reactome; R-CEL-9617828; FOXO-mediated transcription of cell cycle genes.
DR SignaLink; F5GUE5; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00000910; Expressed in larva and 3 other tissues.
DR ExpressionAtlas; F5GUE5; baseline and differential.
DR GO; GO:0071144; C:heteromeric SMAD protein complex; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0070411; F:I-SMAD binding; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009653; P:anatomical structure morphogenesis; IBA:GO_Central.
DR GO; GO:0030509; P:BMP signaling pathway; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0040024; P:dauer larval development; IMP:WormBase.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR GO; GO:0061067; P:negative regulation of dauer larval development; IMP:WormBase.
DR GO; GO:0018991; P:oviposition; IMP:WormBase.
DR GO; GO:0043476; P:pigment accumulation; IMP:WormBase.
DR GO; GO:0061065; P:regulation of dauer larval development; IMP:UniProtKB.
DR GO; GO:0060395; P:SMAD protein signal transduction; IBA:GO_Central.
DR GO; GO:0035176; P:social behavior; IMP:WormBase.
DR GO; GO:0007179; P:transforming growth factor beta receptor signaling pathway; IBA:GO_Central.
DR Gene3D; 2.60.200.10; -; 1.
DR InterPro; IPR013790; Dwarfin.
DR InterPro; IPR017855; SMAD-like_dom_sf.
DR InterPro; IPR001132; SMAD_dom_Dwarfin-type.
DR InterPro; IPR008984; SMAD_FHA_dom_sf.
DR PANTHER; PTHR13703; PTHR13703; 1.
DR Pfam; PF03166; MH2; 1.
DR SMART; SM00524; DWB; 1.
DR SUPFAM; SSF49879; SSF49879; 1.
DR PROSITE; PS51076; MH2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..331
FT /note="Smad-related protein daf-14"
FT /id="PRO_0000452405"
FT DOMAIN 134..331
FT /note="MH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00439"
FT REGION 168..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..44
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_060999"
FT MUTAGEN 236..331
FT /note="Missing: In m77; constitutive dauer formation at 25
FT degrees Celsius, an egg laying defect (Egl), abnormal
FT social aggregation - also known as clumping behavior (Cpy),
FT and a dark intestine (Din)."
FT /evidence="ECO:0000269|PubMed:10625546,
FT ECO:0000269|PubMed:20081192"
FT MUTAGEN 286
FT /note="G->E: In sa340; constitutive dauer formation at 25
FT degrees Celsius, an egg laying defect (Egl), abnormal
FT social aggregation - also known as clumping behavior (Cpy),
FT and a dark intestine (Din)."
FT /evidence="ECO:0000269|PubMed:10625546"
FT MUTAGEN 302..331
FT /note="Missing: In sa535; constitutive dauer formation at
FT 25 degrees Celsius, an egg laying defect (Egl), abnormal
FT social aggregation - also known as clumping behavior (Cpy),
FT and a dark intestine (Din)."
FT /evidence="ECO:0000269|PubMed:10625546"
SQ SEQUENCE 331 AA; 37590 MW; 5F827F3E45FAC185 CRC64;
MSNEQEDFGS LFNNQGELGI MDDFAEFGFQ TTTTPTNWAA AGNYMYPDQV HLPASINNPN
MPINDWLEDA PMPDCYNVPS TSTDENNDPF PFSNISSQSS LKPKTPEKAV VEVRPTGNEM
LDPEPKYPKE EKPWCTIFYY ELTVRLGKAF EAKVPTITID GATGASDECR MSLTSQPSSR
NSKSSQIRNT VGAGIQLAYE NGELWLTVLT DQIVFVQCPF LNQTLNKPLK YVFRLQNKGD
QKRMKIFDKE QFEQEKTLAL GPLTEKEVAD ERMRIFSNIR VSFCKGFGET YSRLKVVNLP
CWIEIILHEP ADEYDTVFRI NNERPEIGSR S
