DAF19_CAEEL
ID DAF19_CAEEL Reviewed; 805 AA.
AC Q09555; C0P280; G5EBW8; Q9NHC9; S6EZR7;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 27-APR-2001, sequence version 3.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=RFX-like transcription factor daf-19;
DE AltName: Full=Abnormal dauer formation protein 19 {ECO:0000312|WormBase:F33H1.1b};
GN Name=daf-19 {ECO:0000312|WormBase:F33H1.1b};
GN ORFNames=F33H1.1 {ECO:0000312|WormBase:F33H1.1b};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B).
RX PubMed=10882127; DOI=10.1016/s1097-2765(00)80436-0;
RA Swoboda P., Adler H.T., Thomas J.H.;
RT "The RFX-type transcription factor DAF-19 regulates sensory neuron cilium
RT formation in C. elegans.";
RL Mol. Cell 5:411-421(2000).
RN [2] {ECO:0000312|EMBL:ACF17932.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), FUNCTION (ISOFORM C), SUBCELLULAR
RP LOCATION (ISOFORM C), TISSUE SPECIFICITY (ISOFORM C), AND MUTAGENESIS OF
RP 255-ARG--LEU-805.
RX PubMed=20923979; DOI=10.1534/genetics.110.122879;
RA Wang J., Schwartz H.T., Barr M.M.;
RT "Functional specialization of sensory cilia by an RFX transcription factor
RT isoform.";
RL Genetics 186:1295-1307(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP 255-ARG--LEU-805.
RX PubMed=12954713; DOI=10.1242/dev.00678;
RA Yu H., Pretot R.F., Burglin T.R., Sternberg P.W.;
RT "Distinct roles of transcription factors EGL-46 and DAF-19 in specifying
RT the functionality of a polycystin-expressing sensory neuron necessary for
RT C. elegans male vulva location behavior.";
RL Development 130:5217-5227(2003).
RN [5]
RP FUNCTION.
RX PubMed=23505381; DOI=10.1371/journal.pgen.1003324;
RA Xie Y., Moussaif M., Choi S., Xu L., Sze J.Y.;
RT "RFX transcription factor DAF-19 regulates 5-HT and innate immune responses
RT to pathogenic bacteria in Caenorhabditis elegans.";
RL PLoS Genet. 9:e1003324-e1003324(2013).
CC -!- FUNCTION: Probable transcription factor (PubMed:10882127,
CC PubMed:12954713). May regulate some genes of ciliated sensory neurons
CC (PubMed:10882127, PubMed:12954713). May activate the expression of the
CC shared components of sensory cilia, but not the cell-type-specific
CC expression (PubMed:10882127, PubMed:12954713). Together with
CC transcription factor atf-7, involved in regulation of the serotonergic
CC response of ADF neurons to pathogenic food (PubMed:23505381).
CC {ECO:0000269|PubMed:10882127, ECO:0000269|PubMed:12954713,
CC ECO:0000269|PubMed:23505381}.
CC -!- FUNCTION: [Isoform c]: Involved in male mating behavior; may play a
CC role in functional specialization of PKD ciliated sensory neurons.
CC {ECO:0000269|PubMed:20923979}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00858,
CC ECO:0000269|PubMed:12954713}.
CC -!- SUBCELLULAR LOCATION: [Isoform c]: Nucleus
CC {ECO:0000269|PubMed:20923979}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Name=b;
CC IsoId=Q09555-1; Sequence=Displayed;
CC Name=a;
CC IsoId=Q09555-2; Sequence=VSP_005661;
CC Name=c {ECO:0000312|WormBase:F33H1.1c}; Synonyms=daf-19m
CC {ECO:0000303|PubMed:20923979};
CC IsoId=Q09555-3; Sequence=VSP_061389;
CC Name=d {ECO:0000312|WormBase:F33H1.1d}; Synonyms=daf-19c
CC {ECO:0000303|PubMed:20923979};
CC IsoId=Q09555-4; Sequence=VSP_061391;
CC Name=e {ECO:0000312|WormBase:F33H1.1e};
CC IsoId=Q09555-5; Sequence=VSP_061390;
CC -!- TISSUE SPECIFICITY: Ciliated sensory neurons.
CC -!- TISSUE SPECIFICITY: [Isoform c]: Expressed in the male tail HOB and RnB
CC neurons but not in male-specific CEM head neurons or other ciliated
CC neurons. {ECO:0000269|PubMed:20923979}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the male-specific ciliated sensory
CC neurons, including the two hook neurons, and the 36 ray neurons at the
CC L4 larval stage. {ECO:0000269|PubMed:12954713}.
CC -!- SIMILARITY: Belongs to the RFX family. {ECO:0000255|PROSITE-
CC ProRule:PRU00858}.
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DR EMBL; AF226156; AAF61564.1; -; mRNA.
DR EMBL; AF233652; AAF63475.1; -; mRNA.
DR EMBL; EU812221; ACF17932.1; -; mRNA.
DR EMBL; Z48783; CAA88701.1; -; Genomic_DNA.
DR EMBL; Z48783; CAC42295.1; -; Genomic_DNA.
DR EMBL; BX284602; CAR97827.1; -; Genomic_DNA.
DR EMBL; BX284602; CAX51691.2; -; Genomic_DNA.
DR EMBL; BX284602; CDG24134.1; -; Genomic_DNA.
DR PIR; T21708; T21708.
DR RefSeq; NP_001022139.1; NM_001026968.3. [Q09555-2]
DR RefSeq; NP_001022140.1; NM_001026969.3. [Q09555-1]
DR AlphaFoldDB; Q09555; -.
DR SMR; Q09555; -.
DR BioGRID; 39896; 4.
DR IntAct; Q09555; 2.
DR STRING; 6239.F33H1.1b; -.
DR EPD; Q09555; -.
DR PaxDb; Q09555; -.
DR PRIDE; Q09555; -.
DR EnsemblMetazoa; F33H1.1a.1; F33H1.1a.1; WBGene00000914. [Q09555-2]
DR EnsemblMetazoa; F33H1.1b.1; F33H1.1b.1; WBGene00000914. [Q09555-1]
DR EnsemblMetazoa; F33H1.1c.1; F33H1.1c.1; WBGene00000914. [Q09555-3]
DR EnsemblMetazoa; F33H1.1d.1; F33H1.1d.1; WBGene00000914. [Q09555-4]
DR EnsemblMetazoa; F33H1.1e.1; F33H1.1e.1; WBGene00000914. [Q09555-5]
DR GeneID; 174577; -.
DR KEGG; cel:CELE_F33H1.1; -.
DR UCSC; F33H1.1b; c. elegans. [Q09555-1]
DR CTD; 174577; -.
DR WormBase; F33H1.1a; CE17763; WBGene00000914; daf-19. [Q09555-2]
DR WormBase; F33H1.1b; CE28019; WBGene00000914; daf-19. [Q09555-1]
DR WormBase; F33H1.1c; CE01567; WBGene00000914; daf-19.
DR WormBase; F33H1.1d; CE43609; WBGene00000914; daf-19.
DR WormBase; F33H1.1e; CE02205; WBGene00000914; daf-19.
DR eggNOG; KOG3712; Eukaryota.
DR GeneTree; ENSGT01050000244879; -.
DR HOGENOM; CLU_010393_2_0_1; -.
DR InParanoid; Q09555; -.
DR OMA; HCEEHRM; -.
DR OrthoDB; 346630at2759; -.
DR PhylomeDB; Q09555; -.
DR PRO; PR:Q09555; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00000914; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR ExpressionAtlas; Q09555; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:UniProtKB.
DR GO; GO:0060179; P:male mating behavior; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0042427; P:serotonin biosynthetic process; IMP:UniProtKB.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR003150; DNA-bd_RFX.
DR InterPro; IPR039779; RFX-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12619; PTHR12619; 1.
DR Pfam; PF02257; RFX_DNA_binding; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS51526; RFX_DBD; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..805
FT /note="RFX-like transcription factor daf-19"
FT /id="PRO_0000215294"
FT DNA_BIND 260..334
FT /note="RFX-type winged-helix"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00858"
FT REGION 1..113
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..89
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..104
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..194
FT /note="MTNEEPVPSTSSVLSAAEKNVKIETPSRKRKGLDIDALWRKKFKDVENQKSP
FT EKSEKSEKTIKEEPLETHPSGERKLRSASKDSKSLSKETHNTISTRSSSSGTPRKKMEP
FT EDVKPNIKMLKKSLPVSFQCSNLNDGTVGDASVMLDPTKISLHSSSVAYGEESQDEMEV
FT IQHSTDDPNGTREEFDYNQIEYGN -> MYLPQCNTMYS (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_061389"
FT VAR_SEQ 1..167
FT /note="Missing (in isoform e)"
FT /evidence="ECO:0000305"
FT /id="VSP_061390"
FT VAR_SEQ 1..144
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000305"
FT /id="VSP_061391"
FT VAR_SEQ 142..166
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000303|PubMed:10882127"
FT /id="VSP_005661"
FT MUTAGEN 255..805
FT /note="Missing: In m86; abolishes expression of ceh-26 and
FT nlp-8 in the male-specific hook neuron HOB. Substantially
FT reduces non-sex-specific expression of ceh-26 in some head
FT neurons. Reduces male-specific expression of pkd-2 in the
FT four ciliated CEM neurons in the head, and the HOB and B-
FT type ray neurons in the tail. Causes constitutive formation
FT of dauer larvae."
FT /evidence="ECO:0000269|PubMed:12954713,
FT ECO:0000269|PubMed:20923979"
SQ SEQUENCE 805 AA; 90908 MW; AAA6D16E7DF768F4 CRC64;
MTNEEPVPST SSVLSAAEKN VKIETPSRKR KGLDIDALWR KKFKDVENQK SPEKSEKSEK
TIKEEPLETH PSGERKLRSA SKDSKSLSKE THNTISTRSS SSGTPRKKME PEDVKPNIKM
LKKSLPVSFQ CSNLNDGTVG DASVMLDPTK ISLHSSSVAY GEESQDEMEV IQHSTDDPNG
TREEFDYNQI EYGNAVTPNG TYTLYAPDSN YAPYYQYSNN MQSSPQDNAP YLVPAPQSPV
SIDDADSINL PNNQRASPAT VNWLFENYEI GEGSLPRCEL YDHYKKHCAE HRMDPVNAAS
FGKLIRSVFH NLKTRRLGTR GNSKYHYYGI RLKDSSTLHS MQHPQPQIIQ NTYTPPMQLP
QRDAYADTVN QVAAIKYMDV EIPDKRARKD NSSSSSSCRD SVSPCMDLPA QQTIQHTPVH
SVIPSTQQPA PLHNNNVVSY VVTESDKAAM GKIDLPIVPF PDKDALMATI GFRKLGVGEE
ELNSLIDIYE ILCREILALI KNIDFASVED TWSKFWSGNF GVDRDHISAL CTLDQVQDYI
IEVDLALYQT IVDTLIPNVL LSELSTGMTQ TCRTFAKNID VYLRKSLMLA NLGEFFVKKK
IQAIKYLQQG LKRYTSLNHL AHASRGVLMK PEQVQQMYQD YIRVDINTVH QQAGWICGCD
SVMVHHVNNA FKHNLQRMSA MEVWAEWLES IVDQVLAKYH DKPANVIANV GKQFLLNWSF
YTSMIIRDLT LRSAMSFGSF TLIRLLADDY MYYLIESKIA KAGKQQLITV IRADKDWPLT
TNPQEYIVVA NDNDEDLDLE KAGLL
