DAF3_CAEEL
ID DAF3_CAEEL Reviewed; 892 AA.
AC Q95QI7; A0A0K3ASQ1; A0A0K3ATA0; A0A0K3AVK9; A0A0K3AVL3; A0A0K3AWB3;
AC A0A0K3AYJ4; O17532; Q95QI8;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Smad protein daf-3 {ECO:0000303|PubMed:9334330, ECO:0000303|PubMed:9834189};
DE AltName: Full=Abnormal dauer formation protein 3 {ECO:0000312|WormBase:F25E2.5a};
GN Name=daf-3 {ECO:0000312|WormBase:F25E2.5a};
GN ORFNames=F25E2.5 {ECO:0000312|WormBase:F25E2.5a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2] {ECO:0000312|EMBL:AAB61748.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-105 (ISOFORM B), FUNCTION, SUBCELLULAR
RP LOCATION, AND MUTAGENESIS OF PRO-296 AND GLY-716.
RX PubMed=9334330; DOI=10.1101/gad.11.20.2679;
RA Patterson G.I., Koweek A., Wong A., Liu Y., Ruvkun G.;
RT "The DAF-3 Smad protein antagonizes TGF-beta-related receptor signaling in
RT the Caenorhabditis elegans dauer pathway.";
RL Genes Dev. 11:2679-2690(1997).
RN [3] {ECO:0000305}
RP FUNCTION.
RX PubMed=9834189; DOI=10.1242/dev.126.1.97;
RA Thatcher J.D., Haun C., Okkema P.G.;
RT "The DAF-3 Smad binds DNA and represses gene expression in the
RT Caenorhabditis elegans pharynx.";
RL Development 126:97-107(1999).
RN [4] {ECO:0000305}
RP FUNCTION, AND INTERACTION WITH DAF-5.
RX PubMed=14681186; DOI=10.1242/dev.00922;
RA da Graca L.S., Zimmerman K.K., Mitchell M.C., Kozhan-Gorodetska M.,
RA Sekiewicz K., Morales Y., Patterson G.I.;
RT "DAF-5 is a Ski oncoprotein homolog that functions in a neuronal TGF beta
RT pathway to regulate C. elegans dauer development.";
RL Development 131:435-446(2004).
RN [5] {ECO:0000305}
RP FUNCTION, AND INTERACTION WITH DAF-8 AND DAF-14.
RX PubMed=20081192; DOI=10.1242/dev.043752;
RA Park D., Estevez A., Riddle D.L.;
RT "Antagonistic Smad transcription factors control the dauer/non-dauer switch
RT in C. elegans.";
RL Development 137:477-485(2010).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=28811311; DOI=10.1242/dev.147660;
RA Pekar O., Ow M.C., Hui K.Y., Noyes M.B., Hall S.E., Hubbard E.J.A.;
RT "Linking the environment, DAF-7/TGFbeta signaling and LAG-2/DSL ligand
RT expression in the germline stem cell niche.";
RL Development 144:2896-2906(2017).
CC -!- FUNCTION: Transcriptional regulator and common SMAD (co-SMAD), required
CC to regulate entry into a developmentally arrested larval state known as
CC dauer, in response to harsh environmental conditions (PubMed:9334330).
CC Probable component of transcriptional regulatory complex with SMAD
CC protein daf-5 (PubMed:14681186). Acts antagonistically to SMAD
CC signaling downstream of TGF-beta-like daf-7 signaling
CC (PubMed:20081192). Binds to the 5'-GTCTG-3' motif found in regulatory
CC regions and may modulate the expression of genes involved in TGF-beta-
CC like daf-7 and Notch lag-2 signaling (PubMed:20081192, PubMed:9834189,
CC PubMed:28811311). May regulate gene expression outside the dauer
CC pathway (PubMed:9834189). {ECO:0000269|PubMed:14681186,
CC ECO:0000269|PubMed:20081192, ECO:0000269|PubMed:28811311,
CC ECO:0000269|PubMed:9334330, ECO:0000269|PubMed:9834189}.
CC -!- SUBUNIT: Interacts with R-SMADs daf-8 and daf-14 (PubMed:20081192).
CC Interacts with daf-14 in a daf-8 dependent manner (PubMed:20081192).
CC May interact with daf-5 (PubMed:14681186).
CC {ECO:0000269|PubMed:14681186, ECO:0000269|PubMed:20081192}.
CC -!- INTERACTION:
CC Q95QI7; G5EDM7: daf-5; NbExp=4; IntAct=EBI-326363, EBI-360260;
CC Q95QI7; Q9XVS0: fath-1; NbExp=2; IntAct=EBI-326363, EBI-360227;
CC Q95QI7; G5EF87: swsn-1; NbExp=2; IntAct=EBI-326363, EBI-311928;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|RuleBase:RU361195,
CC ECO:0000269|PubMed:9334330}. Nucleus {ECO:0000255|RuleBase:RU361195,
CC ECO:0000269|PubMed:9334330}. Chromosome {ECO:0000269|PubMed:9334330}.
CC Note=Localization observed during mitosis.
CC {ECO:0000269|PubMed:9334330}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=9;
CC Name=a {ECO:0000312|WormBase:F25E2.5a};
CC IsoId=Q95QI7-1; Sequence=Displayed;
CC Name=d {ECO:0000312|WormBase:F25E2.5d};
CC IsoId=Q95QI7-2; Sequence=VSP_060998;
CC Name=f {ECO:0000312|WormBase:F25E2.5f};
CC IsoId=Q95QI7-3; Sequence=VSP_060996;
CC Name=e {ECO:0000312|WormBase:F25E2.5e};
CC IsoId=Q95QI7-4; Sequence=VSP_060996, VSP_060998;
CC Name=b {ECO:0000312|WormBase:F25E2.5b};
CC IsoId=Q95QI7-5; Sequence=VSP_060996, VSP_060997;
CC Name=c {ECO:0000312|WormBase:F25E2.5c};
CC IsoId=Q95QI7-6; Sequence=VSP_060995;
CC Name=g {ECO:0000312|WormBase:F25E2.5g};
CC IsoId=Q95QI7-7; Sequence=VSP_060994, VSP_060998;
CC Name=h {ECO:0000312|WormBase:F25E2.5h};
CC IsoId=Q95QI7-8; Sequence=VSP_060994;
CC Name=i {ECO:0000312|WormBase:F25E2.5i};
CC IsoId=Q95QI7-9; Sequence=VSP_060993;
CC -!- SIMILARITY: Belongs to the dwarfin/SMAD family.
CC {ECO:0000255|RuleBase:RU361195}.
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DR EMBL; AF005205; AAB61748.1; -; mRNA.
DR EMBL; BX284606; CCD69986.1; -; Genomic_DNA.
DR EMBL; BX284606; CCD69987.1; -; Genomic_DNA.
DR EMBL; BX284606; CCD69988.1; -; Genomic_DNA.
DR EMBL; BX284606; CTQ87052.1; -; Genomic_DNA.
DR EMBL; BX284606; CTQ87053.1; -; Genomic_DNA.
DR EMBL; BX284606; CTQ87054.1; -; Genomic_DNA.
DR EMBL; BX284606; CTQ87055.1; -; Genomic_DNA.
DR EMBL; BX284606; CTQ87056.1; -; Genomic_DNA.
DR EMBL; BX284606; CTQ87057.1; -; Genomic_DNA.
DR RefSeq; NP_001024604.1; NM_001029433.3. [Q95QI7-5]
DR RefSeq; NP_001024605.1; NM_001029434.2. [Q95QI7-6]
DR RefSeq; NP_001300341.1; NM_001313412.1. [Q95QI7-2]
DR RefSeq; NP_001300342.1; NM_001313413.1. [Q95QI7-4]
DR RefSeq; NP_001300343.1; NM_001313414.1. [Q95QI7-3]
DR RefSeq; NP_001300344.1; NM_001313415.1. [Q95QI7-7]
DR RefSeq; NP_001300345.1; NM_001313416.1.
DR RefSeq; NP_001300346.1; NM_001313417.1. [Q95QI7-9]
DR RefSeq; NP_508161.3; NM_075760.4. [Q95QI7-1]
DR AlphaFoldDB; Q95QI7; -.
DR SMR; Q95QI7; -.
DR DIP; DIP-26559N; -.
DR IntAct; Q95QI7; 15.
DR STRING; 6239.F25E2.5a; -.
DR EPD; Q95QI7; -.
DR PaxDb; Q95QI7; -.
DR PeptideAtlas; Q95QI7; -.
DR EnsemblMetazoa; F25E2.5a.1; F25E2.5a.1; WBGene00000899. [Q95QI7-1]
DR EnsemblMetazoa; F25E2.5b.1; F25E2.5b.1; WBGene00000899. [Q95QI7-5]
DR EnsemblMetazoa; F25E2.5c.1; F25E2.5c.1; WBGene00000899. [Q95QI7-6]
DR EnsemblMetazoa; F25E2.5d.1; F25E2.5d.1; WBGene00000899. [Q95QI7-2]
DR EnsemblMetazoa; F25E2.5e.1; F25E2.5e.1; WBGene00000899. [Q95QI7-4]
DR EnsemblMetazoa; F25E2.5f.1; F25E2.5f.1; WBGene00000899. [Q95QI7-3]
DR EnsemblMetazoa; F25E2.5g.1; F25E2.5g.1; WBGene00000899. [Q95QI7-7]
DR EnsemblMetazoa; F25E2.5h.1; F25E2.5h.1; WBGene00000899. [Q95QI7-8]
DR EnsemblMetazoa; F25E2.5i.1; F25E2.5i.1; WBGene00000899. [Q95QI7-9]
DR GeneID; 180431; -.
DR KEGG; cel:CELE_F25E2.5; -.
DR UCSC; F25E2.5c; c. elegans.
DR CTD; 180431; -.
DR WormBase; F25E2.5a; CE28002; WBGene00000899; daf-3.
DR WormBase; F25E2.5b; CE30963; WBGene00000899; daf-3.
DR WormBase; F25E2.5c; CE26364; WBGene00000899; daf-3.
DR WormBase; F25E2.5d; CE50706; WBGene00000899; daf-3.
DR WormBase; F25E2.5e; CE50732; WBGene00000899; daf-3.
DR WormBase; F25E2.5f; CE28003; WBGene00000899; daf-3.
DR WormBase; F25E2.5g; CE50852; WBGene00000899; daf-3.
DR WormBase; F25E2.5h; CE50691; WBGene00000899; daf-3.
DR WormBase; F25E2.5i; CE50737; WBGene00000899; daf-3.
DR eggNOG; KOG3701; Eukaryota.
DR HOGENOM; CLU_323960_0_0_1; -.
DR InParanoid; Q95QI7; -.
DR OMA; HCKHAFE; -.
DR OrthoDB; 949229at2759; -.
DR PhylomeDB; Q95QI7; -.
DR SignaLink; Q95QI7; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00000899; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR ExpressionAtlas; Q95QI7; baseline and differential.
DR GO; GO:0000793; C:condensed chromosome; IDA:WormBase.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0005667; C:transcription regulator complex; IEA:InterPro.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0071444; P:cellular response to pheromone; IGI:UniProtKB.
DR GO; GO:0040024; P:dauer larval development; IMP:WormBase.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IGI:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IGI:WormBase.
DR GO; GO:0061066; P:positive regulation of dauer larval development; IMP:WormBase.
DR GO; GO:0061065; P:regulation of dauer larval development; IGI:UniProtKB.
DR GO; GO:0043051; P:regulation of pharyngeal pumping; IGI:WormBase.
DR GO; GO:0007179; P:transforming growth factor beta receptor signaling pathway; IEA:InterPro.
DR Gene3D; 2.60.200.10; -; 1.
DR Gene3D; 3.90.520.10; -; 1.
DR InterPro; IPR013790; Dwarfin.
DR InterPro; IPR003619; MAD_homology1_Dwarfin-type.
DR InterPro; IPR013019; MAD_homology_MH1.
DR InterPro; IPR017855; SMAD-like_dom_sf.
DR InterPro; IPR001132; SMAD_dom_Dwarfin-type.
DR InterPro; IPR008984; SMAD_FHA_dom_sf.
DR InterPro; IPR036578; SMAD_MH1_sf.
DR PANTHER; PTHR13703; PTHR13703; 1.
DR Pfam; PF03165; MH1; 1.
DR Pfam; PF03166; MH2; 1.
DR SMART; SM00523; DWA; 1.
DR SMART; SM00524; DWB; 1.
DR SUPFAM; SSF49879; SSF49879; 1.
DR SUPFAM; SSF56366; SSF56366; 1.
DR PROSITE; PS51075; MH1; 1.
DR PROSITE; PS51076; MH2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; Cytoplasm; DNA-binding; Metal-binding;
KW Nucleus; Reference proteome; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..892
FT /note="Smad protein daf-3"
FT /id="PRO_0000452403"
FT DOMAIN 198..347
FT /note="MH1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00438"
FT DOMAIN 657..880
FT /note="MH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00439"
FT REGION 1..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 135..161
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 505..552
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..29
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..152
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 514..541
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..609
FT /note="Missing (in isoform i)"
FT /evidence="ECO:0000305"
FT /id="VSP_060993"
FT VAR_SEQ 1..112
FT /note="Missing (in isoform g and isoform h)"
FT /evidence="ECO:0000305"
FT /id="VSP_060994"
FT VAR_SEQ 1..105
FT /note="MGDHHNLTGLPGTSIPPQFNYSQPGTSTGGPLYGGKPSHGLEDIPDVEEYER
FT NLLGAGAGFNLLNVGNMANEFKPIITLDTKPPRDANKSLAFNGGLKLITPKTE -> MK
FT LIATSLL (in isoform c)"
FT /id="VSP_060995"
FT VAR_SEQ 72..105
FT /note="Missing (in isoform f, isoform e and isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060996"
FT VAR_SEQ 330
FT /note="M -> MVGEALQ (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060997"
FT VAR_SEQ 330
FT /note="M -> MVGE (in isoform d, isoform e and isoform g)"
FT /evidence="ECO:0000305"
FT /id="VSP_060998"
FT MUTAGEN 296
FT /note="P->L: In mg125; suppresses the constitutive dauer
FT arrest phenotype on a daf-7 mutant background."
FT /evidence="ECO:0000269|PubMed:9334330"
FT MUTAGEN 716
FT /note="G->E: In mg132; suppresses the constitutive dauer
FT arrest phenotype on a daf-7 mutant background."
FT /evidence="ECO:0000269|PubMed:9334330"
SQ SEQUENCE 892 AA; 101059 MW; 088B4E7B6359961F CRC64;
MGDHHNLTGL PGTSIPPQFN YSQPGTSTGG PLYGGKPSHG LEDIPDVEEY ERNLLGAGAG
FNLLNVGNMA NEFKPIITLD TKPPRDANKS LAFNGGLKLI TPKTEVPDEH TPMMSPVNTT
TKILQRSGIK MEIPPYLDPD SQDDDPEDGV NYPDPDLFDT KNTNMTEYDL DVLKLGKPAV
DEARKKIEVP DASAPPNKIV EYLMYYRTLK ESELIQLNAY RTKRNRLSLN LVKNNIDREF
DQKACESLVK KLKDKKNDLQ NLIDVVLSKG TKYTGCITIP RTLDGRLQVH GRKGFPHVVY
GKLWRFNEMT KNETRHVDHC KHAFEMKSDM VCVNPYHYEI VIGTMIVGQR DHDNRDMPPP
HQRYHTPGRQ DPVDDMSRFI PPASIRPPPM NMHTRPQPMP QQLPSVGATF AHPLPHQAPH
NPGVSHPYSI APQTHYPLNM NPIPQMPQMP QMPPPLHQGY GMNGPSCSSE NNNPFHQNHH
YNDISHPNHY SYDCGPNLYG FPTPYPDFHH PFNQQPHQPP QLSQNHTSQQ GSHQPGHQGQ
VPNDPPISRP VLQPSTVTLD VFRRYCRQTF GNRFFEGESE QSGAIIRSSN KFIEEFDSPI
CGVTVVRPRM TDGEVLENIM PEDAPYHDIC KFILRLTSES VTFSGEGPEV SDLNEKWGTI
VYYEKNLQIG EKKCSRGNFH VDGGFICSEN RYSLGLEPNP IREPVAFKVR KAIVDGIRFS
YKKDGSVWLQ NRMKYPVFVT SGYLDEQSGG LKKDKVHKVY GCASIKTFGF NVSKQIIRDA
LLSKQMATMY LQGKLTPMNY IYEKKTQEEL RREATRTTDS LAKYCCVRVS FCKGFGEAYP
ERPSIHDCPV WIELKINIAY DFMDSICQYI TNCFEPLGME DFAKLGINVS DD
