DAF5_CAEEL
ID DAF5_CAEEL Reviewed; 627 AA.
AC G5EDM7;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Ski protein homolog {ECO:0000303|PubMed:14681186};
DE AltName: Full=Abnormal dauer formation protein 5 {ECO:0000312|WormBase:W01G7.1};
GN Name=daf-5 {ECO:0000312|WormBase:W01G7.1};
GN ORFNames=W01G7.1 {ECO:0000312|WormBase:W01G7.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAR00670.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH DAF-3, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND MUTAGENESIS OF GLU-162.
RX PubMed=14681186; DOI=10.1242/dev.00922;
RA da Graca L.S., Zimmerman K.K., Mitchell M.C., Kozhan-Gorodetska M.,
RA Sekiewicz K., Morales Y., Patterson G.I.;
RT "DAF-5 is a Ski oncoprotein homolog that functions in a neuronal TGF beta
RT pathway to regulate C. elegans dauer development.";
RL Development 131:435-446(2004).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Probable component of transcriptional regulatory complex with
CC SMAD protein daf-3 (PubMed:14681186). Required to regulate entry into a
CC developmentally arrested larval state known as dauer, in response to
CC harsh environmental conditions (PubMed:14681186). Involved in larvae
CC undergoing cell-cycle arrest during the dauer stage (PubMed:14681186).
CC {ECO:0000269|PubMed:14681186}.
CC -!- SUBUNIT: May interact with daf-3. {ECO:0000269|PubMed:14681186}.
CC -!- INTERACTION:
CC G5EDM7; Q95QI7: daf-3; NbExp=4; IntAct=EBI-360260, EBI-326363;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14681186}.
CC -!- TISSUE SPECIFICITY: Expressed in ganglia in the head and tail and in
CC the anterior pharynx. {ECO:0000269|PubMed:14681186}.
CC -!- SIMILARITY: Belongs to the SKI family. {ECO:0000305}.
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DR EMBL; AY438643; AAR00670.1; -; mRNA.
DR EMBL; BX284602; CAB03453.1; -; Genomic_DNA.
DR PIR; T26064; T26064.
DR RefSeq; NP_496941.1; NM_064540.5.
DR AlphaFoldDB; G5EDM7; -.
DR IntAct; G5EDM7; 1.
DR STRING; 6239.W01G7.1; -.
DR EPD; G5EDM7; -.
DR PaxDb; G5EDM7; -.
DR EnsemblMetazoa; W01G7.1.1; W01G7.1.1; WBGene00000901.
DR EnsemblMetazoa; W01G7.1.2; W01G7.1.2; WBGene00000901.
DR GeneID; 175054; -.
DR KEGG; cel:CELE_W01G7.1; -.
DR CTD; 175054; -.
DR WormBase; W01G7.1; CE18985; WBGene00000901; daf-5.
DR eggNOG; ENOG502TFH6; Eukaryota.
DR HOGENOM; CLU_385063_0_0_1; -.
DR InParanoid; G5EDM7; -.
DR OMA; GYWYKNR; -.
DR OrthoDB; 1358876at2759; -.
DR Reactome; R-CEL-201451; Signaling by BMP.
DR Reactome; R-CEL-2173795; Downregulation of SMAD2/3:SMAD4 transcriptional activity.
DR SignaLink; G5EDM7; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00000901; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0046332; F:SMAD binding; IBA:GO_Central.
DR GO; GO:0040024; P:dauer larval development; IGI:WormBase.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IGI:WormBase.
DR GO; GO:0061066; P:positive regulation of dauer larval development; IMP:WormBase.
DR GO; GO:0007179; P:transforming growth factor beta receptor signaling pathway; IGI:WormBase.
DR Gene3D; 3.10.390.10; -; 1.
DR InterPro; IPR014890; c-SKI_SMAD4-bd_dom.
DR InterPro; IPR010919; SAND-like_dom_sf.
DR InterPro; IPR023216; Tscrpt_reg_SKI_SnoN.
DR PANTHER; PTHR10005; PTHR10005; 2.
DR Pfam; PF08782; c-SKI_SMAD_bind; 1.
DR SUPFAM; SSF63763; SSF63763; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome.
FT CHAIN 1..627
FT /note="Ski protein homolog"
FT /id="PRO_0000452406"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 34..58
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..318
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..15
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 162
FT /note="E->K: In sa310; suppression of constitutive dauer
FT formation phenotype on a daf-8; daf-14 double mutant
FT background."
FT /evidence="ECO:0000269|PubMed:14681186"
SQ SEQUENCE 627 AA; 71099 MW; 69409A5F6968EDEF CRC64;
MSDSPIGSSQ QVEPEHRTPD LMDIDPLIAN LKALHEETRS DDDDDGQPST SAKRKDSRAD
GIVIHQKKYS DPGRFLWIWL LGVRVPALSI DGEPHLPIEI LDDMLTKKDK KDQMSFQNLL
RYKNVYIRMA SPSQFRAVME KSKECENLNI TSLSLMSRSD IERIMGELRL ESMLTLAEHD
NWDISDRVHV VHVNFIDYCS EWLESDDLEE DVMQSGTHGY WYKNRRNMRC IECQHCEGKF
TPTDFIMHHH YPIKPSGFVH TGCNSFQWIR LIEVFDKSNE NLEAWNKFVL NSHRAGKREY
DEAAPHQAPP KRPAMETPVP VAADNGWEAD EEEEGEEIVD RDADIEKCKL RNKKKMENLH
IADFLGPSGS KGLKPRNKFE AVIIEQLNKM DDAALEALFL KSPEEYNLWV KESDFTHKVV
TQQQEWKAKM KDPNFKSRAS ANFDVSKGEF DNMRHFDNAS KATRQEIQQL AEQFANLDRD
AKLLTPMEFV LREHALLKNV SADAIRVLCN RPPLPPLPPP PPPPKPKPAP VQPISLGNIN
FVALAQQLIA SGIKLPLPIV TPPVVSTPAP VITPIPAALP ISPNSDFLKQ QLSTAMSSPA
LLSLYPKLTA GAYEQLAQFI KTTTVKN
