DAF7_CAEEL
ID DAF7_CAEEL Reviewed; 350 AA.
AC P92172;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Dauer larva development regulatory growth factor daf-7;
DE AltName: Full=Abnormal dauer formation protein 7;
DE Flags: Precursor;
GN Name=daf-7 {ECO:0000312|WormBase:B0412.2};
GN ORFNames=B0412.2 {ECO:0000312|WormBase:B0412.2};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, INDUCTION, AND MUTAGENESIS OF PRO-271 AND GLY-280.
RC STRAIN=Bristol N2;
RX PubMed=8910282; DOI=10.1126/science.274.5291.1389;
RA Ren P., Lim C.-S., Johnsen R., Albert P.S., Pilgrim D., Riddle D.L.;
RT "Control of C. elegans larval development by neuronal expression of a TGF-
RT beta homolog.";
RL Science 274:1389-1391(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION.
RX PubMed=8413626; DOI=10.1038/365644a0;
RA Estevez M., Attisano L., Wrana J.L., Albert P.S., Massague J., Riddle D.L.;
RT "The daf-4 gene encodes a bone morphogenetic protein receptor controlling
RT C. elegans dauer larva development.";
RL Nature 365:644-649(1993).
RN [4]
RP FUNCTION.
RX PubMed=18524955; DOI=10.1073/pnas.0707469105;
RA Hallem E.A., Sternberg P.W.;
RT "Acute carbon dioxide avoidance in Caenorhabditis elegans.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:8038-8043(2008).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF 105-GLN--SER-350.
RX PubMed=20713521; DOI=10.1101/gad.1932610;
RA Lesch B.J., Bargmann C.I.;
RT "The homeodomain protein hmbx-1 maintains asymmetric gene expression in
RT adult C. elegans olfactory neurons.";
RL Genes Dev. 24:1802-1815(2010).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=30024377; DOI=10.7554/elife.36547;
RA Hilbert Z.A., Kim D.H.;
RT "PDF-1 neuropeptide signaling regulates sexually dimorphic gene expression
RT in shared sensory neurons of C. elegans.";
RL Elife 7:0-0(2018).
RN [7]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=31264582; DOI=10.7554/elife.43660;
RA Lawson H., Vuong E., Miller R.M., Kiontke K., Fitch D.H., Portman D.S.;
RT "The Makorin lep-2 and the lncRNA lep-5 regulate lin-28 to schedule sexual
RT maturation of the C. elegans nervous system.";
RL Elife 8:0-0(2019).
CC -!- FUNCTION: Under harsh environmental conditions, larvae enter a
CC developmentally arrested state known as dauer; TGF-beta-like daf-7 acts
CC to inhibit dauer larva formation and promote growth (PubMed:8910282).
CC May be a ligand to cell surface receptor daf-4 (PubMed:8413626). May
CC act as a negative regulator of dauer larva development by transducing
CC chemosensory information from ASI neurons (PubMed:8910282). Involved in
CC sensitivity to CO2 levels (PubMed:18524955). Involved in mate searching
CC behavior of males, acting in concert with the neuropeptide pdf-1
CC (PubMed:30024377). In AWC neurons, acts to promote expression of srsx-
CC 3, a member of the GPCR family (PubMed:20713521).
CC {ECO:0000269|PubMed:18524955, ECO:0000269|PubMed:20713521,
CC ECO:0000269|PubMed:30024377, ECO:0000269|PubMed:8413626,
CC ECO:0000269|PubMed:8910282}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the chemosensory neurons, including in
CC the ASJ neurons in males. {ECO:0000269|PubMed:30024377,
CC ECO:0000269|PubMed:31264582, ECO:0000269|PubMed:8910282}.
CC -!- DEVELOPMENTAL STAGE: When the food/pheromone ratio is high, its level
CC peaks during the L1 larval stage (PubMed:8910282). Expression is
CC detected in larvae beginning 4 to 5 hours after hatching, through the
CC four larval stages, and in adults (PubMed:8910282). Expressed in ASJ
CC chemosensory neurons in males from the L4 larval stage
CC (PubMed:31264582). {ECO:0000269|PubMed:30024377,
CC ECO:0000269|PubMed:8910282}.
CC -!- INDUCTION: Dauer-inducing pheromone inhibits its expression and
CC promotes dauer formation, whereas food reactivates its expression and
CC promotes recovery from the dauer state. {ECO:0000269|PubMed:8910282}.
CC -!- SIMILARITY: Belongs to the TGF-beta family. {ECO:0000305}.
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DR EMBL; U72883; AAC47390.1; -; mRNA.
DR EMBL; U72884; AAC47389.1; -; Genomic_DNA.
DR EMBL; BX284603; CCD61866.1; -; Genomic_DNA.
DR PIR; T25451; T25451.
DR RefSeq; NP_497265.1; NM_064864.4.
DR AlphaFoldDB; P92172; -.
DR BioGRID; 40506; 1.
DR STRING; 6239.B0412.2; -.
DR EPD; P92172; -.
DR PaxDb; P92172; -.
DR PeptideAtlas; P92172; -.
DR EnsemblMetazoa; B0412.2.1; B0412.2.1; WBGene00000903.
DR GeneID; 175237; -.
DR KEGG; cel:CELE_B0412.2; -.
DR UCSC; B0412.2; c. elegans.
DR CTD; 175237; -.
DR WormBase; B0412.2; CE01758; WBGene00000903; daf-7.
DR eggNOG; KOG3900; Eukaryota.
DR HOGENOM; CLU_020515_6_0_1; -.
DR InParanoid; P92172; -.
DR OMA; ETPLCCR; -.
DR OrthoDB; 892873at2759; -.
DR PhylomeDB; P92172; -.
DR Reactome; R-CEL-381426; Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
DR Reactome; R-CEL-8957275; Post-translational protein phosphorylation.
DR SignaLink; P92172; -.
DR PRO; PR:P92172; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00000903; Expressed in sensory neuron and 24 other tissues.
DR GO; GO:0005615; C:extracellular space; IDA:WormBase.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0005160; F:transforming growth factor beta receptor binding; ISS:WormBase.
DR GO; GO:0071321; P:cellular response to cGMP; IMP:UniProtKB.
DR GO; GO:0071444; P:cellular response to pheromone; IGI:UniProtKB.
DR GO; GO:0043053; P:dauer entry; IGI:UniProtKB.
DR GO; GO:0040024; P:dauer larval development; IMP:WormBase.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:WormBase.
DR GO; GO:0016045; P:detection of bacterium; IMP:UniProtKB.
DR GO; GO:0032490; P:detection of molecule of bacterial origin; IMP:UniProtKB.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR GO; GO:0030536; P:larval feeding behavior; IMP:UniProtKB.
DR GO; GO:0061067; P:negative regulation of dauer larval development; IMP:WormBase.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0010862; P:positive regulation of pathway-restricted SMAD protein phosphorylation; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0061065; P:regulation of dauer larval development; IMP:UniProtKB.
DR GO; GO:1903998; P:regulation of eating behavior; IMP:UniProtKB.
DR GO; GO:0043051; P:regulation of pharyngeal pumping; IMP:WormBase.
DR GO; GO:0009266; P:response to temperature stimulus; IMP:UniProtKB.
DR GO; GO:0060395; P:SMAD protein signal transduction; IBA:GO_Central.
DR GO; GO:0007179; P:transforming growth factor beta receptor signaling pathway; ISS:WormBase.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR001839; TGF-b_C.
DR InterPro; IPR016319; TGF-beta.
DR InterPro; IPR015615; TGF-beta-rel.
DR InterPro; IPR017948; TGFb_CS.
DR PANTHER; PTHR11848; PTHR11848; 1.
DR Pfam; PF00019; TGF_beta; 1.
DR PIRSF; PIRSF001787; TGF-beta; 1.
DR SMART; SM00204; TGFB; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
DR PROSITE; PS00250; TGF_BETA_1; 1.
DR PROSITE; PS51362; TGF_BETA_2; 1.
PE 1: Evidence at protein level;
KW Cleavage on pair of basic residues; Developmental protein; Disulfide bond;
KW Glycoprotein; Growth factor; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT PROPEP 22..234
FT /evidence="ECO:0000255"
FT /id="PRO_0000033674"
FT CHAIN 235..350
FT /note="Dauer larva development regulatory growth factor
FT daf-7"
FT /id="PRO_0000033675"
FT CARBOHYD 23
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 241..251
FT /evidence="ECO:0000250"
FT DISULFID 250..315
FT /evidence="ECO:0000250"
FT DISULFID 278..347
FT /evidence="ECO:0000250"
FT DISULFID 282..349
FT /evidence="ECO:0000250"
FT DISULFID 314
FT /note="Interchain"
FT /evidence="ECO:0000250"
FT MUTAGEN 105..350
FT /note="Missing: In ky771; reduces expression of the G
FT protein-coupled receptor (GPCR) srsx-3 in the AWC neuron."
FT /evidence="ECO:0000269|PubMed:20713521"
FT MUTAGEN 271
FT /note="P->S: In E1372; loss of function."
FT /evidence="ECO:0000269|PubMed:8910282"
FT MUTAGEN 280
FT /note="G->R: In M70; loss of function."
FT /evidence="ECO:0000269|PubMed:8910282"
SQ SEQUENCE 350 AA; 39533 MW; F957C70A2B1FDE0A CRC64;
MFMASSLPVF IFLLSLPHGL TFNCTNSGVC IEKMKQHRTE YLKNEILDQL NMKEAPKGLK
PMDPEMKSVY LEMYRDLLEK DEQDMGVEMS FYTAKDPSYG ENPSQLVAKF DVTNDLERSD
ILQATLTVSI EIPAKDSGML QDVQVQVYEK NEDGSMGEMV TSGIFATKGS ERISIQLPID
TVKSWFTISP IQGIFVKAML DGRNVALHPQ QTTADVDNMR LQLSTRPKGS RKRRSHAKPV
CNAEAQSKGC CLYDLEIEFE KIGWDWIVAP PRYNAYMCRG DCHYNAHHFN LAETGHSKIM
RAAHKVSNPE IGYCCHPTEY DYIKLIYVNR DGRVSIANVN GMIAKKCGCS
