ID   ACT22_STRCO             Reviewed;         578 AA.
AC   P46105;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 116.
DE   RecName: Full=Probable actinorhodin transporter;
GN   Name=actII-2; OrderedLocusNames=SCO5083; ORFNames=SCBAC28G1.09;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1878971; DOI=10.1016/0092-8674(91)90120-n;
RA   Fernandez-Moreno M.A., Caballero J.L., Hopwood D.A., Malpartida F.;
RT   "The act cluster contains regulatory and antibiotic export genes, direct
RT   targets for translational control by the bldA tRNA gene of Streptomyces.";
RL   Cell 66:769-780(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
CC   -!- FUNCTION: Promotes the efflux of actinorhodin.
CC   -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. EmrB family.
CC       {ECO:0000305}.
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DR   EMBL; M64683; AAA26690.1; -; Genomic_DNA.
DR   EMBL; AL939122; CAC44196.1; -; Genomic_DNA.
DR   PIR; B40046; B40046.
DR   RefSeq; NP_629233.1; NC_003888.3.
DR   RefSeq; WP_011030046.1; NZ_VNID01000008.1.
DR   AlphaFoldDB; P46105; -.
DR   SMR; P46105; -.
DR   STRING; 100226.SCO5083; -.
DR   TCDB; 2.A.1.3.25; the major facilitator superfamily (mfs).
DR   GeneID; 1100524; -.
DR   KEGG; sco:SCO5083; -.
DR   PATRIC; fig|100226.15.peg.5163; -.
DR   eggNOG; COG0477; Bacteria.
DR   HOGENOM; CLU_000960_28_2_11; -.
DR   InParanoid; P46105; -.
DR   OMA; GMFGPVM; -.
DR   PhylomeDB; P46105; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016020; C:membrane; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..578
FT                   /note="Probable actinorhodin transporter"
FT                   /id="PRO_0000173313"
FT   TRANSMEM        78..98
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        109..129
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        135..155
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        202..222
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        232..252
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        259..279
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        306..326
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        341..361
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        369..389
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        444..464
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        546..566
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..33
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   578 AA;  59772 MW;  E6C1DC75E6038B92 CRC64;
     MSSVEADEPD RATAPPSALL PEDGPGPDGT AAGPPPYARR WAALGVILGA EIMDLLDGTV
     MNVAAPAVRA DLGGSLSVIQ WITVGYTLAF AVLLVVGGRL GDIYGRKRMF VVGAVGFTAA
     SVLCSVAAGP EMLTAARFLQ GGLGALMIPQ GLGLIKQMFP PKETAAAFGA FGPAIGLGAV
     LGPIVAGFLV DADLFGTGWR SVFLINLPIG VAVIVGAVLL LPEGKAPVRP KFDVVGMALV
     TSGLTLLIFP LVQGRERGWP AWAFVLMLAG AAVLVGFVAH ELRQERRGGA TLIELSLLRR
     SRYAAGLAVA LVFFTGVSGM SLLLALHLQI GLGFSPTRAA LTMTPWSVFL VVGAILTGAV
     LGSKFGRKAL HGGLVVLALG VLIMLLTIGD QAGGLTSWEL VPGIAVAGLG MGIMIGLLFD
     IALADVDKQE AGTASGVLTA VQQLGFTVGV AVLGTLFFGL LGSQATASVD DGASRARTEL
     AAAGASTTEQ DRLLADLRVC LRESASQQDS ERTPDSCRNL QQARPAVAEA TARAWRTAHT
     ENFSTAMVRT LWVVIALLAV SFALAFRLPP KPREEEGF