ID   DAIP_STRMB              Reviewed;         374 AA.
AC   P84908; N1NV52;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2013, sequence version 2.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Dispase autolysis-inducing protein {ECO:0000303|PubMed:19420706};
DE   Flags: Precursor;
GN   Name=daip;
OS   Streptomyces mobaraensis (Streptoverticillium mobaraense).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=35621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29032 / CBS 199.75 / DSM 40847 / NBRC 13819 / NCIMB 11159 /
RC   NRRL B-3729 / VKM Ac-928;
RA   Zindel S., Froels S., Kletzin A., Fiebig D., Pfeifer F., Fuchsbauer H.L.;
RT   "Gene structure of the dispase autolysis-inducing protein from Streptomyces
RT   mobraensis.";
RL   Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PROTEIN SEQUENCE OF 27-49, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=19420706; DOI=10.1271/bbb.80769;
RA   Sarafeddinov A., Schmidt S., Adolf F., Mainusch M., Bender A.,
RA   Fuchsbauer H.-L.;
RT   "A novel transglutaminase substrate from Streptomyces mobaraensis triggers
RT   autolysis of neutral metalloproteases.";
RL   Biosci. Biotechnol. Biochem. 73:993-999(2009).
CC   -!- FUNCTION: Induces autolysis of dispase and thermolysin.
CC       {ECO:0000269|PubMed:19420706}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19420706}.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 7.1,
CC       its MW is: 39 kDa. {ECO:0000269|PubMed:19420706}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; HF968455; CCW72537.1; -; Genomic_DNA.
DR   PDB; 5FZP; X-ray; 1.70 A; A/B=27-374.
DR   PDB; 6FHP; X-ray; 1.70 A; A/B=31-372.
DR   PDBsum; 5FZP; -.
DR   PDBsum; 6FHP; -.
DR   AlphaFoldDB; P84908; -.
DR   SMR; P84908; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   Gene3D; 2.130.10.10; -; 2.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000269|PubMed:19420706"
FT   CHAIN           27..374
FT                   /note="Dispase autolysis-inducing protein"
FT                   /id="PRO_0000370231"
FT   STRAND          39..52
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          60..62
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          66..68
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          72..75
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          82..86
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          89..95
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          101..105
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          112..115
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   TURN            117..119
FT                   /evidence="ECO:0007829|PDB:6FHP"
FT   STRAND          120..124
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          131..135
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          138..141
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          147..154
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          162..166
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          171..176
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          182..185
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          195..201
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          204..215
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          217..222
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          231..236
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          241..248
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          256..262
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   TURN            263..266
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          275..286
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          288..292
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          295..297
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          305..307
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          314..321
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   HELIX           323..325
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          327..334
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   TURN            335..337
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          340..355
FT                   /evidence="ECO:0007829|PDB:5FZP"
FT   STRAND          362..369
FT                   /evidence="ECO:0007829|PDB:5FZP"
SQ   SEQUENCE   374 AA;  38811 MW;  BCF16F8484A9EE36 CRC64;
     MKRMGWAVTA AVTTIVLAQS SLAAQAADST SGWRAPSCTK VTGDGAVTFT TDDGATLAPT
     TGTLQSVSYT HGLVALDTPN TLLATHNDEL QRSTDAGCTW TKVATLGSGS TWLTAATGGR
     AFAWEKNGGY LARVDGRTVT KLSSPSADIV GVGTDKARRD HVRLAGSDGQ LYDSTDAGAT
     WKPLGKLAFG PGASVYTVSF DPADLDHAVA GGMTTGGAVT TDGGATWTAA TGLSATAGGK
     SNLFAASVSP ADRNVVYALG IDLVEAAPNS GAEGRHLYRS TDGGRTYTRI VDDTPDTELT
     NSTLLAPSPV DPNVLYFEYG TYFQAYGTDL YRYDARTGKV GKTHNAHDGI SAIAFNPARP
     SVMYLGLEEV QIHH