DAL81_YEAST
ID DAL81_YEAST Reviewed; 970 AA.
AC P21657; D6VVV4;
DT 01-MAY-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 3.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Transcriptional activator protein DAL81;
DE AltName: Full=Regulatory protein UGA35;
GN Name=DAL81; Synonyms=DURL, UGA35; OrderedLocusNames=YIR023W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1999281; DOI=10.1016/0378-1119(91)90048-g;
RA Coornaert D., Vissers S., Andre B.;
RT "The pleiotropic UGA35(DURL) regulatory gene of Saccharomyces cerevisiae:
RT cloning, sequence and identity with the DAL81 gene.";
RL Gene 97:163-171(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1990272; DOI=10.1128/mcb.11.2.1161-1166.1991;
RA Bricmont P.A., Daugherty J.R., Cooper T.G.;
RT "The DAL81 gene product is required for induced expression of two
RT differently regulated nitrogen catabolic genes in Saccharomyces
RT cerevisiae.";
RL Mol. Cell. Biol. 11:1161-1166(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-833, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Positive regulation of genes required for catabolism of GABA
CC (UGA4, UGA1, and UGA2), urea (DUR1 and DUR2), arginine and allantoin.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 704 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; M63498; AAA35192.1; -; Genomic_DNA.
DR EMBL; M60415; AAA34557.1; -; Genomic_DNA.
DR EMBL; Z38061; CAA86183.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08570.1; -; Genomic_DNA.
DR PIR; S48485; S48485.
DR RefSeq; NP_012289.3; NM_001179545.3.
DR AlphaFoldDB; P21657; -.
DR BioGRID; 35014; 580.
DR DIP; DIP-5733N; -.
DR IntAct; P21657; 5.
DR MINT; P21657; -.
DR STRING; 4932.YIR023W; -.
DR iPTMnet; P21657; -.
DR MaxQB; P21657; -.
DR PaxDb; P21657; -.
DR PRIDE; P21657; -.
DR EnsemblFungi; YIR023W_mRNA; YIR023W; YIR023W.
DR GeneID; 854841; -.
DR KEGG; sce:YIR023W; -.
DR SGD; S000001462; DAL81.
DR VEuPathDB; FungiDB:YIR023W; -.
DR eggNOG; ENOG502QQXX; Eukaryota.
DR HOGENOM; CLU_006632_0_0_1; -.
DR InParanoid; P21657; -.
DR OMA; MQDKWSS; -.
DR BioCyc; YEAST:G3O-31442-MON; -.
DR PRO; PR:P21657; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P21657; protein.
DR GO; GO:0005634; C:nucleus; IC:SGD.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0003713; F:transcription coactivator activity; IDA:SGD.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0001080; P:nitrogen catabolite activation of transcription from RNA polymerase II promoter; IMP:SGD.
DR GO; GO:1901717; P:positive regulation of gamma-aminobutyric acid catabolic process; IMP:SGD.
DR GO; GO:1901714; P:positive regulation of urea catabolic process; IMP:SGD.
DR GO; GO:0051123; P:RNA polymerase II preinitiation complex assembly; IDA:SGD.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR007219; Transcription_factor_dom_fun.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF04082; Fungal_trans; 1.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00906; Fungal_trans; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Metal-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..970
FT /note="Transcriptional activator protein DAL81"
FT /id="PRO_0000114947"
FT DNA_BIND 150..179
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 64..95
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 118..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 807..970
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..95
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 807..909
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 910..935
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 936..970
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 833
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT CONFLICT 77..82
FT /note="Missing (in Ref. 1; AAA35192)"
FT /evidence="ECO:0000305"
FT CONFLICT 465
FT /note="K -> R (in Ref. 1; AAA35192)"
FT /evidence="ECO:0000305"
FT CONFLICT 497
FT /note="S -> L (in Ref. 1; AAA35192)"
FT /evidence="ECO:0000305"
FT CONFLICT 507
FT /note="D -> G (in Ref. 1; AAA35192)"
FT /evidence="ECO:0000305"
FT CONFLICT 760
FT /note="N -> S (in Ref. 1; AAA35192)"
FT /evidence="ECO:0000305"
FT CONFLICT 785
FT /note="T -> A (in Ref. 2; AAA34557)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 970 AA; 109174 MW; 9FEBD271A18AFC38 CRC64;
MDPHQSPADN AASPTKSVKA TTKNSSTNNN VNSNNSNNNS NHDILNFNDN YTTILQHLAN
DHPNILREKG GSQQQQHQQQ QQQQQQQQQQ QQQQSLDTLL HHYQSLLSKS DNAIAFDDNV
SNSADHNGSN SNNNNNNNDI SSPGNLMGSC NQCRLKKTKC NYFPDLGNCL ECETSRTKCT
FSIAPNYLKR TSSGANNNMP TSSNSKRMKN FEDYSNRLPS SMLYRHQQQQ QQQQQQQRIQ
YPRSSFFVGP ASVFDLNLTK HVRLDNVDQI QLSKTLSLRK VSPTAQFILQ DDFDTTLHSK
QEYEVDLVEN LVHPHGHLLV EIFFKLIHPF LPILHERVFL EKYSRSYREL TAPLLASIYS
LALQYWDFHP ALLGFPKPDV TAQLNNIALE TFYARVGRPK LSIIQTGLLI LQCRSECHNN
WVLCSSVVAL AEELGLGVEC NDWKLPKWEK DLRKRLAWAV WLMDKWCALN EGRQSHLILG
RNWMIKLLNF DDFPLNSPTI LNSLQNDQSG SSPSSSNDVK NHQIAFGNLP IFNINPTLED
FKNGTLMFQQ MVSLSIILGE IMDTFYTQGS MTINKSIEQV LKLAKPLQLK LREWYHSLPK
NLSMSYATPQ KLNSNSTLTL AYFATEITLH RKIICALNPQ TPKELVQVCR TAARTRLVAA
IEFIRDLKNE HINAFWYNCS TGNLMLIGTF AALLYVTSAT KEEAMIFRDY VRNYTWVLKI
GSKYFDKLSN ALNNMHLLFA QIPGLLTDEP VVVSPNSNIN SVNPQRSGVQ SQIPIQFNVG
SPAMTEQGSP LNQWKNLPQE ILQQLNSFPN GTTSTTTPVN PTSRQTQLES QGSPAINSAN
NNSNNTPLPF APNKSSKKTS QSSPNVTPSH MSRHPPSNTS SPRVNSSTNV NSNTQMNASP
LTSINETRQE SGDAADEKTA GRERTANEES STELKDDNPN SNQETSATGN QTIKMNDDKN
VTINTRETPL
