DAM1_ASHGO
ID DAM1_ASHGO Reviewed; 339 AA.
AC Q75EQ0;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 2.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=DASH complex subunit DAM1;
DE AltName: Full=Outer kinetochore protein DAM1;
GN Name=DAM1; OrderedLocusNames=AAR029W;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO C-TERMINUS.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC subcomplex of the outer kinetochore that is essential for proper
CC chromosome segregation. The DASH complex mediates the formation and
CC maintenance of bipolar kinetochore-microtubule attachments by forming
CC closed rings around spindle microtubules and establishing interactions
CC with proteins from the central kinetochore (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC microtubules. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Cytoplasm, cytoskeleton,
CC spindle {ECO:0000250}. Chromosome, centromere, kinetochore
CC {ECO:0000250}. Note=Associates with the mitotic spindle and the
CC kinetochore. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DASH complex DAM1 family. {ECO:0000305}.
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DR EMBL; AE016814; AAS50394.2; -; Genomic_DNA.
DR RefSeq; NP_982570.2; NM_207923.2.
DR AlphaFoldDB; Q75EQ0; -.
DR STRING; 33169.AAS50394; -.
DR EnsemblFungi; AAS50394; AAS50394; AGOS_AAR029W.
DR GeneID; 4618458; -.
DR KEGG; ago:AGOS_AAR029W; -.
DR eggNOG; ENOG502S08R; Eukaryota.
DR HOGENOM; CLU_065404_0_0_1; -.
DR InParanoid; Q75EQ0; -.
DR OMA; LYGLMCN; -.
DR Proteomes; UP000000591; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0042729; C:DASH complex; IBA:GO_Central.
DR GO; GO:1990537; C:mitotic spindle polar microtubule; IBA:GO_Central.
DR GO; GO:0044732; C:mitotic spindle pole body; IBA:GO_Central.
DR GO; GO:0042802; F:identical protein binding; IEA:EnsemblFungi.
DR GO; GO:0051010; F:microtubule plus-end binding; IEA:EnsemblFungi.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:1990758; P:mitotic sister chromatid biorientation; IBA:GO_Central.
DR GO; GO:0051987; P:positive regulation of attachment of spindle microtubules to kinetochore; IEA:EnsemblFungi.
DR GO; GO:0031116; P:positive regulation of microtubule polymerization; IEA:EnsemblFungi.
DR InterPro; IPR013962; DASH_Dam1.
DR PANTHER; PTHR28113; PTHR28113; 1.
DR Pfam; PF08653; DASH_Dam1; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Coiled coil; Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis;
KW Nucleus; Reference proteome.
FT CHAIN 1..339
FT /note="DASH complex subunit DAM1"
FT /id="PRO_0000127656"
FT REGION 1..48
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 157..179
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 205..224
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 286..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 120..150
FT /evidence="ECO:0000255"
FT COMPBIAS 14..48
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..329
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 339 AA; 36698 MW; 23F92A4389C3F0AE CRC64;
MSKESVRESK AATEYRLSIS SNPGSRRSSF GGSSEHPAGS GIGNNKASNG ELREATLVDT
LLLPQVQELK DSMITLDANL THMNFIHESL VDLNESVSAL LYGLMCNSWC VDFPNMPHHT
ARELGISKEL ARLKEEKQQL LADLQGTAQA PSLVLKEKEP NTSKQKFQLP KPPMVSTRSV
VAPVRTISEE EEDDNTAASF VSNPTVMGQP PHAPPPVARS NNAKGRRRYS ILQQIRNHDL
TGQKHMVANL GGTVKARAAT HPIPESAGEK RKSLAVSAVR IGNNKRLQTS RPPSGGRDVT
MARKRSGTQP AILNTNSITH SSTGAVPASS AQGRRPPFR
