DAM1_SCHPO
ID DAM1_SCHPO Reviewed; 155 AA.
AC Q9HDZ6;
DT 21-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=DASH complex subunit dam1;
DE AltName: Full=Outer kinetochore protein dam1;
GN Name=dam1; ORFNames=SPAC589.08c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=16079914; DOI=10.1038/sj.emboj.7600762;
RA Liu X., McLeod I., Anderson S., Yates J.R. III, He X.;
RT "Molecular analysis of kinetochore architecture in fission yeast.";
RL EMBO J. 24:2919-2930(2005).
CC -!- FUNCTION: Component of the DASH complex, a microtubule-binding
CC subcomplex of the outer kinetochore that is essential for proper
CC chromosome segregation. The DASH complex mediates the formation and
CC maintenance of bipolar kinetochore-microtubule attachments by forming
CC closed rings around spindle microtubules and establishing interactions
CC with proteins from the central kinetochore.
CC {ECO:0000269|PubMed:16079914}.
CC -!- SUBUNIT: The DASH complex oligomerizes to form rings that encircle the
CC microtubules. Integrity of the complex and interactions with central
CC kinetochore proteins are regulated by the spindle assembly checkpoint
CC kinase ark1. {ECO:0000269|PubMed:16079914}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16079914}. Cytoplasm,
CC cytoskeleton, spindle {ECO:0000269|PubMed:16079914}. Chromosome,
CC centromere, kinetochore {ECO:0000269|PubMed:16079914}. Note=Associates
CC with the mitotic spindle and the kinetochore. Kinetochore association
CC occurs only during mitosis.
CC -!- SIMILARITY: Belongs to the DASH complex DAM1 family. {ECO:0000305}.
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DR EMBL; CU329670; CAC19765.1; -; Genomic_DNA.
DR RefSeq; NP_594056.1; NM_001019480.2.
DR AlphaFoldDB; Q9HDZ6; -.
DR SMR; Q9HDZ6; -.
DR BioGRID; 278948; 145.
DR IntAct; Q9HDZ6; 3.
DR STRING; 4896.SPAC589.08c.1; -.
DR iPTMnet; Q9HDZ6; -.
DR MaxQB; Q9HDZ6; -.
DR PaxDb; Q9HDZ6; -.
DR EnsemblFungi; SPAC589.08c.1; SPAC589.08c.1:pep; SPAC589.08c.
DR GeneID; 2542489; -.
DR KEGG; spo:SPAC589.08c; -.
DR PomBase; SPAC589.08c; dam1.
DR VEuPathDB; FungiDB:SPAC589.08c; -.
DR eggNOG; ENOG502S08R; Eukaryota.
DR HOGENOM; CLU_1687699_0_0_1; -.
DR InParanoid; Q9HDZ6; -.
DR OMA; QINAFCV; -.
DR PhylomeDB; Q9HDZ6; -.
DR PRO; PR:Q9HDZ6; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0055028; C:cortical microtubule; IDA:PomBase.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0042729; C:DASH complex; IDA:PomBase.
DR GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR GO; GO:0072686; C:mitotic spindle; HDA:PomBase.
DR GO; GO:1990537; C:mitotic spindle polar microtubule; IDA:PomBase.
DR GO; GO:0044732; C:mitotic spindle pole body; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0051315; P:attachment of mitotic spindle microtubules to kinetochore; IGI:PomBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0007019; P:microtubule depolymerization; IMP:PomBase.
DR GO; GO:1990942; P:mitotic metaphase chromosome recapture; IMP:PomBase.
DR GO; GO:1990758; P:mitotic sister chromatid biorientation; IMP:PomBase.
DR GO; GO:0051455; P:monopolar spindle attachment to meiosis I kinetochore; IC:PomBase.
DR InterPro; IPR013962; DASH_Dam1.
DR PANTHER; PTHR28113; PTHR28113; 1.
DR Pfam; PF08653; DASH_Dam1; 1.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Cytoplasm; Cytoskeleton; Kinetochore; Microtubule; Mitosis; Nucleus;
KW Reference proteome.
FT CHAIN 1..155
FT /note="DASH complex subunit dam1"
FT /id="PRO_0000127661"
SQ SEQUENCE 155 AA; 17444 MW; BED2A5612EBE2D85 CRC64;
MEKYQKATQN PLENVDNVKI ESENAIPSNL QAFTKSLAVL DDNVSEFRKR MNHLSATKQI
LDNFNESFSS FLYGLQINAF CVDYENAPLS ESFLLQAKKD QFKATLMTRT GHSISDPPYD
GGVISHDPNF ATADETFATN DTSFIERPET YSASR
