DAND5_MOUSE
ID DAND5_MOUSE Reviewed; 185 AA.
AC Q76LW6;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=DAN domain family member 5;
DE AltName: Full=Cerberus-like protein 2;
DE Short=Cerl-2;
DE Flags: Precursor;
GN Name=Dand5; Synonyms=Cerl2, Sp1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=C57BL/6J;
RX PubMed=15466485; DOI=10.1101/gad.306504;
RA Marques S., Borges A.C., Silva A.C., Freitas S., Cordenonsi M., Belo J.A.;
RT "The activity of the Nodal antagonist Cerl-2 in the mouse node is required
RT for correct L/R body axis.";
RL Genes Dev. 18:2342-2347(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Itoh N.;
RT "Cysteine knot protein (SP1).";
RL Submitted (AUG-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Seems to play a role in the correct specification of the
CC left-right axis. May antagonize NODAL and BMP4 signaling. Cystine knot-
CC containing proteins play important roles during development,
CC organogenesis, and tissue growth and differentiation.
CC {ECO:0000269|PubMed:15466485}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: At 7.0 dpc (early head-fold stage) expressed in
CC perinodal region. At 7.5 dpc (late head-fold stage) expression begins
CC to decrease in intensity on the left side and starts to be
CC asymmetrically up-regulated on the right side of the node. By early 8.0
CC dpc (somitogenesis), highly expressed in the right side of the node. No
CC expression seen in later stages of development.
CC {ECO:0000269|PubMed:15466485}.
CC -!- DISRUPTION PHENOTYPE: Mice display multiple laterality defects
CC including randomization of the L/R axis, left pulmonary isomerism
CC thoracic situs inversus and cardiovascular malformations.
CC {ECO:0000269|PubMed:15466485}.
CC -!- SIMILARITY: Belongs to the DAN family. {ECO:0000305}.
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DR EMBL; AY387409; AAR25615.1; -; mRNA.
DR EMBL; AB070694; BAC82439.1; -; mRNA.
DR EMBL; BC115659; AAI15660.1; -; mRNA.
DR CCDS; CCDS22477.1; -.
DR RefSeq; NP_957679.1; NM_201227.3.
DR AlphaFoldDB; Q76LW6; -.
DR SMR; Q76LW6; -.
DR BioGRID; 204764; 1.
DR STRING; 10090.ENSMUSP00000070057; -.
DR GlyGen; Q76LW6; 1 site.
DR PhosphoSitePlus; Q76LW6; -.
DR PaxDb; Q76LW6; -.
DR PRIDE; Q76LW6; -.
DR Antibodypedia; 26335; 288 antibodies from 22 providers.
DR DNASU; 23863; -.
DR Ensembl; ENSMUST00000065539; ENSMUSP00000070057; ENSMUSG00000053226.
DR GeneID; 23863; -.
DR KEGG; mmu:23863; -.
DR UCSC; uc009mnh.1; mouse.
DR CTD; 199699; -.
DR MGI; MGI:1344365; Dand5.
DR VEuPathDB; HostDB:ENSMUSG00000053226; -.
DR eggNOG; ENOG502RZ3T; Eukaryota.
DR GeneTree; ENSGT00530000063926; -.
DR HOGENOM; CLU_122900_0_0_1; -.
DR InParanoid; Q76LW6; -.
DR OMA; PVVLWCR; -.
DR OrthoDB; 1134947at2759; -.
DR PhylomeDB; Q76LW6; -.
DR TreeFam; TF106445; -.
DR BioGRID-ORCS; 23863; 6 hits in 71 CRISPR screens.
DR ChiTaRS; Dand5; mouse.
DR PRO; PR:Q76LW6; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q76LW6; protein.
DR Bgee; ENSMUSG00000053226; Expressed in primitive knot and 105 other tissues.
DR Genevisible; Q76LW6; MM.
DR GO; GO:0005576; C:extracellular region; NAS:BHF-UCL.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0016015; F:morphogen activity; IMP:BHF-UCL.
DR GO; GO:0003283; P:atrial septum development; IMP:BHF-UCL.
DR GO; GO:0061371; P:determination of heart left/right asymmetry; IMP:BHF-UCL.
DR GO; GO:0003140; P:determination of left/right asymmetry in lateral mesoderm; IMP:BHF-UCL.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:MGI.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; IDA:BHF-UCL.
DR GO; GO:1900108; P:negative regulation of nodal signaling pathway; IDA:BHF-UCL.
DR GO; GO:1900176; P:negative regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry; IBA:GO_Central.
DR GO; GO:1900146; P:negative regulation of nodal signaling pathway involved in determination of left/right asymmetry; ISS:BHF-UCL.
DR GO; GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IGI:MGI.
DR GO; GO:1900164; P:nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry; NAS:BHF-UCL.
DR GO; GO:0017015; P:regulation of transforming growth factor beta receptor signaling pathway; IDA:MGI.
DR GO; GO:0035582; P:sequestering of BMP in extracellular matrix; IBA:GO_Central.
DR GO; GO:0038101; P:sequestering of nodal from receptor via nodal binding; IDA:BHF-UCL.
DR GO; GO:0023019; P:signal transduction involved in regulation of gene expression; IBA:GO_Central.
DR GO; GO:0003281; P:ventricular septum development; IMP:BHF-UCL.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR016860; Cerberus.
DR InterPro; IPR006207; Cys_knot_C.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR004133; DAN.
DR PANTHER; PTHR15273; PTHR15273; 1.
DR Pfam; PF03045; DAN; 1.
DR PIRSF; PIRSF027807; Cerberus; 1.
DR SMART; SM00041; CT; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..185
FT /note="DAN domain family member 5"
FT /id="PRO_0000311805"
FT DOMAIN 97..182
FT /note="CTCK"
FT CARBOHYD 39
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 97..144
FT /evidence="ECO:0000250|UniProtKB:O60565"
FT DISULFID 111..158
FT /evidence="ECO:0000250|UniProtKB:O60565"
FT DISULFID 121..179
FT /evidence="ECO:0000250|UniProtKB:O60565"
FT DISULFID 125..181
FT /evidence="ECO:0000250|UniProtKB:O60565"
SQ SEQUENCE 185 AA; 19772 MW; 520A745E246AF4DA CRC64;
MFRSQFTTLL GLFSGAWLPT GSGRPGAPAT PVQSGTAINQ SWTLDPLVPI SALGSWEAFL
GLQNKQQGTG ELQGGGQRVA AGVPLPLAPQ EVLQETCKAL SFVQVISRPG CTSARVLNHL
CFGRCSSFYI PSSDPTPVVF CNSCVPARKR WTSVTLWCGA GQLASPRRVR ISTVLVQKCQ
CRPKL
