ID   DAND5_XENTR             Reviewed;         219 AA.
AC   Q28H35; Q28GV2;
DT   04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   03-AUG-2022, entry version 46.
DE   RecName: Full=DAN domain family member 5;
DE   AltName: Full=Cerberus-like 2 protein;
DE            Short=Cerl-2;
DE   Flags: Precursor;
GN   Name=dand5; Synonyms=cerl2, coco; ORFNames=TEgg007d24.1, TEgg050n09.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Egg;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays an important role in regulating the left-right axis by
CC       blocking a tgfb1 cascade in the right posterior paraxial mesoderm.
CC       Functions as an inhibitor of bmp, tgfb1, nodal, activin and wnt
CC       signaling in the ectoderm. May inhibit mesodermal signals, probably
CC       through an inhibition of nodal/activin pathways. Seems to regulates
CC       cell fate specification and competence before the onset of neural
CC       induction. Expression in the entire ectodermal region prior to
CC       gastrulation might act to prevent fate specification in the ectoderm
CC       and ensure the maintenance of the stem-cell-like properties exhibited
CC       by ectodermal cells (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with nr1-a. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DAN family. {ECO:0000305}.
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DR   EMBL; CR761214; CAJ81707.1; -; mRNA.
DR   EMBL; CR761079; CAJ82131.1; -; mRNA.
DR   RefSeq; NP_001037924.1; NM_001044459.1.
DR   RefSeq; NP_001037962.1; NM_001044497.1.
DR   AlphaFoldDB; Q28H35; -.
DR   GeneID; 733727; -.
DR   KEGG; xtr:733727; -.
DR   CTD; 199699; -.
DR   Xenbase; XB-GENE-480809; dand5.
DR   InParanoid; Q28H35; -.
DR   OrthoDB; 1134947at2759; -.
DR   Proteomes; UP000008143; Chromosome 3.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR   GO; GO:0016015; F:morphogen activity; IBA:GO_Central.
DR   GO; GO:0061371; P:determination of heart left/right asymmetry; IBA:GO_Central.
DR   GO; GO:1900176; P:negative regulation of nodal signaling pathway involved in determination of lateral mesoderm left/right asymmetry; IBA:GO_Central.
DR   GO; GO:0035582; P:sequestering of BMP in extracellular matrix; IBA:GO_Central.
DR   GO; GO:0023019; P:signal transduction involved in regulation of gene expression; IBA:GO_Central.
DR   Gene3D; 2.10.90.10; -; 1.
DR   InterPro; IPR016860; Cerberus.
DR   InterPro; IPR006207; Cys_knot_C.
DR   InterPro; IPR029034; Cystine-knot_cytokine.
DR   InterPro; IPR004133; DAN.
DR   PANTHER; PTHR15273; PTHR15273; 1.
DR   Pfam; PF03045; DAN; 1.
DR   PIRSF; PIRSF027807; Cerberus; 1.
DR   SMART; SM00041; CT; 1.
DR   PROSITE; PS01225; CTCK_2; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..219
FT                   /note="DAN domain family member 5"
FT                   /id="PRO_0000311807"
FT   DOMAIN          118..198
FT                   /note="CTCK"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        118..165
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        132..179
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        142..195
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   DISULFID        146..197
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00039"
FT   CONFLICT        24
FT                   /note="D -> N (in Ref. 1; CAJ81707)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   219 AA;  25031 MW;  3A23F4DE34CD05BB CRC64;
     MLLFRAASLL PLLCFTVGAF PFMDEEGSGS FAQEVLHSRS YPLANAHRGP FVDLPLFRQN
     RRKISQNFIL HSDPREHMDE EALRRKLVWE SAIRREKMRS HPDQVLPIGQ DALKRSRCHA
     LPFIQNVFRK NCFPVRLPNK FCFGQCNSFY VPGWPAGLSQ PCTSCAPSRS RRISLPLRCR
     AGHLAWQEVE LVEECECETR YDRNTVEPAG SGEGFLPVS