DAP1_MOUSE
ID DAP1_MOUSE Reviewed; 102 AA.
AC Q91XC8;
DT 07-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Death-associated protein 1;
DE Short=DAP-1;
GN Name=Dap;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=129, FVB/N, and FVB/N-3; TISSUE=Colon, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=19131326; DOI=10.1074/mcp.m800451-mcp200;
RA Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.;
RT "Large scale localization of protein phosphorylation by use of electron
RT capture dissociation mass spectrometry.";
RL Mol. Cell. Proteomics 8:904-912(2009).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-29, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: Negative regulator of autophagy. Involved in mediating
CC interferon-gamma-induced cell death (By similarity). {ECO:0000250}.
CC -!- PTM: Phosphorylated. Phosphorylation by MTOR inhibits the suppressive
CC activity of DAP toward autophagy (By similarity). {ECO:0000250}.
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DR EMBL; BC010828; AAH10828.1; -; mRNA.
DR EMBL; BC024876; AAH24876.1; -; mRNA.
DR EMBL; BC057669; AAH57669.1; -; mRNA.
DR CCDS; CCDS27406.1; -.
DR RefSeq; NP_666169.1; NM_146057.3.
DR AlphaFoldDB; Q91XC8; -.
DR BioGRID; 230149; 2.
DR STRING; 10090.ENSMUSP00000047186; -.
DR iPTMnet; Q91XC8; -.
DR PhosphoSitePlus; Q91XC8; -.
DR EPD; Q91XC8; -.
DR jPOST; Q91XC8; -.
DR MaxQB; Q91XC8; -.
DR PaxDb; Q91XC8; -.
DR PRIDE; Q91XC8; -.
DR ProteomicsDB; 279275; -.
DR DNASU; 223453; -.
DR Ensembl; ENSMUST00000044524; ENSMUSP00000047186; ENSMUSG00000039168.
DR GeneID; 223453; -.
DR KEGG; mmu:223453; -.
DR UCSC; uc007vkc.2; mouse.
DR CTD; 1611; -.
DR MGI; MGI:1918190; Dap.
DR VEuPathDB; HostDB:ENSMUSG00000039168; -.
DR eggNOG; ENOG502S4ST; Eukaryota.
DR GeneTree; ENSGT00940000154574; -.
DR HOGENOM; CLU_150759_2_0_1; -.
DR InParanoid; Q91XC8; -.
DR OMA; PTHDARP; -.
DR OrthoDB; 1620337at2759; -.
DR PhylomeDB; Q91XC8; -.
DR TreeFam; TF329716; -.
DR BioGRID-ORCS; 223453; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Dap; mouse.
DR PRO; PR:Q91XC8; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q91XC8; protein.
DR Bgee; ENSMUSG00000039168; Expressed in urinary bladder urothelium and 248 other tissues.
DR ExpressionAtlas; Q91XC8; baseline and differential.
DR Genevisible; Q91XC8; MM.
DR GO; GO:0070513; F:death domain binding; ISO:MGI.
DR GO; GO:0006919; P:activation of cysteine-type endopeptidase activity involved in apoptotic process; ISO:MGI.
DR GO; GO:0006915; P:apoptotic process; ISO:MGI.
DR GO; GO:0097190; P:apoptotic signaling pathway; ISO:MGI.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0034198; P:cellular response to amino acid starvation; ISO:MGI.
DR GO; GO:0010507; P:negative regulation of autophagy; ISO:MGI.
DR GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; ISO:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:MGI.
DR InterPro; IPR024130; DAP1/DAPL1.
DR PANTHER; PTHR13177; PTHR13177; 1.
DR Pfam; PF15228; DAP; 1.
PE 1: Evidence at protein level;
KW Acetylation; Apoptosis; Autophagy; Phosphoprotein; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:P51397"
FT CHAIN 2..102
FT /note="Death-associated protein 1"
FT /id="PRO_0000079783"
FT REGION 1..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..49
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:P51397"
FT MOD_RES 3
FT /note="Phosphoserine; by MTOR"
FT /evidence="ECO:0000250|UniProtKB:P51397"
FT MOD_RES 29
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 49
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P51397"
FT MOD_RES 51
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:19131326, ECO:0007744|PubMed:21183079"
FT MOD_RES 91
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P51397"
SQ SEQUENCE 102 AA; 11155 MW; E6ECD12BEA21A557 CRC64;
MSSPPEGKLE TKAGHPPAVK AGGMRIVQKH PHTGDGKEER DKDDQEWEST SPPKPTVFIS
GVIARGDKDF PPAAAQVAHQ KPHASMDKHV SPRTQHIQQP RK
