DAP1_YEAST
ID DAP1_YEAST Reviewed; 152 AA.
AC Q12091; D6W3J8;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Damage response protein 1;
GN Name=DAP1; OrderedLocusNames=YPL170W; ORFNames=P2515;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204511 / S288c / AB972;
RX PubMed=8948103;
RX DOI=10.1002/(sici)1097-0061(199611)12:14<1483::aid-yea34>3.0.co;2-o;
RA Purnelle B., Coster F., Goffeau A.;
RT "The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a
RT small nuclear RNA, a new putative protein kinase and two new putative
RT regulators.";
RL Yeast 12:1483-1492(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP CHARACTERIZATION.
RX PubMed=12684380; DOI=10.1128/ec.2.2.306-317.2003;
RA Hand R.A., Jia N., Bard M., Craven R.J.;
RT "Saccharomyces cerevisiae Dap1p, a novel DNA damage response protein
RT related to the mammalian membrane-associated progesterone receptor.";
RL Eukaryot. Cell 2:306-317(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- DOMAIN: The cytochrome b5 heme-binding domain lacks the conserved iron-
CC binding His residues at positions 81 and 105. {ECO:0000250}.
CC -!- MISCELLANEOUS: Present with 6300 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the cytochrome b5 family. MAPR subfamily.
CC {ECO:0000305}.
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DR EMBL; X96770; CAA65551.1; -; Genomic_DNA.
DR EMBL; Z73526; CAA97876.1; -; Genomic_DNA.
DR EMBL; AY558521; AAS56847.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11264.1; -; Genomic_DNA.
DR PIR; S65181; S65181.
DR RefSeq; NP_015155.1; NM_001183984.1.
DR AlphaFoldDB; Q12091; -.
DR SMR; Q12091; -.
DR BioGRID; 36013; 106.
DR DIP; DIP-5226N; -.
DR IntAct; Q12091; 4.
DR MINT; Q12091; -.
DR STRING; 4932.YPL170W; -.
DR iPTMnet; Q12091; -.
DR MaxQB; Q12091; -.
DR PaxDb; Q12091; -.
DR PRIDE; Q12091; -.
DR TopDownProteomics; Q12091; -.
DR EnsemblFungi; YPL170W_mRNA; YPL170W; YPL170W.
DR GeneID; 855933; -.
DR KEGG; sce:YPL170W; -.
DR SGD; S000006091; DAP1.
DR VEuPathDB; FungiDB:YPL170W; -.
DR eggNOG; KOG1110; Eukaryota.
DR GeneTree; ENSGT00940000169479; -.
DR HOGENOM; CLU_042860_1_2_1; -.
DR InParanoid; Q12091; -.
DR OMA; YSNFAGH; -.
DR BioCyc; YEAST:G3O-34066-MON; -.
DR Reactome; R-SCE-6798695; Neutrophil degranulation.
DR Reactome; R-SCE-9013405; RHOD GTPase cycle.
DR PRO; PR:Q12091; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; Q12091; protein.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005768; C:endosome; IDA:SGD.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0020037; F:heme binding; IDA:SGD.
DR GO; GO:0032443; P:regulation of ergosterol biosynthetic process; IMP:SGD.
DR Gene3D; 3.10.120.10; -; 1.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR Pfam; PF00173; Cyt-b5; 1.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF55856; SSF55856; 1.
PE 1: Evidence at protein level;
KW Reference proteome.
FT CHAIN 1..152
FT /note="Damage response protein 1"
FT /id="PRO_0000121747"
FT DOMAIN 46..145
FT /note="Cytochrome b5 heme-binding"
FT REGION 18..37
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 152 AA; 16758 MW; 3E3CAC2403C3246F CRC64;
MSFIKNLLFG GVKTSEDPTG LTGNGASNTN DSNKGSEPVV AGNFFPRTLS KFNGHDDEKI
FIAIRGKVYD CTRGRQFYGP SGPYTNFAGH DASRGLALNS FDLDVIKDWD QPIDPLDDLT
KEQIDALDEW QEHFENKYPC IGTLIPEPGV NV
