ID   DAPAL_PYRFU             Reviewed;         298 AA.
AC   Q8U319;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Uncharacterized DapA-like lyase PF0657;
DE            EC=4.-.-.-;
GN   Name=dapAL; OrderedLocusNames=PF0657;
OS   Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=186497;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
RX   PubMed=10430560; DOI=10.1093/genetics/152.4.1299;
RA   Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M.,
RA   DiRuggiero J., Robb F.T.;
RT   "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P.
RT   horikoshii inferred from complete genomic sequences.";
RL   Genetics 152:1299-1305(1999).
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DapA family. {ECO:0000305}.
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DR   EMBL; AE009950; AAL80781.1; -; Genomic_DNA.
DR   RefSeq; WP_011011778.1; NZ_CP023154.1.
DR   AlphaFoldDB; Q8U319; -.
DR   SMR; Q8U319; -.
DR   STRING; 186497.PF0657; -.
DR   EnsemblBacteria; AAL80781; AAL80781; PF0657.
DR   GeneID; 41712459; -.
DR   KEGG; pfu:PF0657; -.
DR   PATRIC; fig|186497.12.peg.690; -.
DR   eggNOG; arCOG04172; Archaea.
DR   HOGENOM; CLU_049343_5_1_2; -.
DR   OMA; YYGYGGF; -.
DR   OrthoDB; 65776at2157; -.
DR   PhylomeDB; Q8U319; -.
DR   Proteomes; UP000001013; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008675; F:2-dehydro-3-deoxy-phosphogluconate aldolase activity; IEA:UniProt.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   InterPro; IPR020625; Schiff_base-form_aldolases_AS.
DR   PANTHER; PTHR12128; PTHR12128; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   PRINTS; PR00146; DHPICSNTHASE.
DR   SMART; SM01130; DHDPS; 1.
DR   PROSITE; PS00666; DHDPS_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lyase; Reference proteome; Schiff base.
FT   CHAIN           1..298
FT                   /note="Uncharacterized DapA-like lyase PF0657"
FT                   /id="PRO_0000103204"
FT   ACT_SITE        43
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        105
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        131
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        159
FT                   /note="Schiff-base intermediate with substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   298 AA;  33751 MW;  90D285C5D03C09E9 CRC64;
     MEGVIVPLVT PFKEDYSIDF EALEWHIEFL EDKGVHGIFT NSTTGEFTSL SFEEKKLLAE
     KGRELTSKAY YLVGTGSTNT FEVIELTKHA KDIGADATVI VSPYYCKLKD EAIFRHFSIV
     AERADIPIIL YAIPSCANPI NVEIVRKLAL EHSNIIGIKA SVDSLTYLGE LLEVKEERKD
     FKVFTGLDQY FFTLLTLGGD GGIMACANFA PEIHLEIWNA FKEKNFRRAI ELARELVRIT
     KIYKIASSFA SAVKLAMVAK GFPIRPVLRP PYVIDGEEVF NEIREIVEWR QMLRKESL