ID   DAPAL_PYRHO             Reviewed;         287 AA.
AC   O58577;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Uncharacterized DapA-like lyase PH0847;
DE            EC=4.-.-.-;
GN   Name=dapAL; OrderedLocusNames=PH0847;
OS   Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS   100139 / OT-3).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=70601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX   PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA   Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA   Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA   Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA   Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA   Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT   "Complete sequence and gene organization of the genome of a hyper-
RT   thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL   DNA Res. 5:55-76(1998).
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DapA family. {ECO:0000305}.
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DR   EMBL; BA000001; BAA29941.1; -; Genomic_DNA.
DR   PIR; C71135; C71135.
DR   RefSeq; WP_010884940.1; NC_000961.1.
DR   AlphaFoldDB; O58577; -.
DR   SMR; O58577; -.
DR   STRING; 70601.3257258; -.
DR   PRIDE; O58577; -.
DR   EnsemblBacteria; BAA29941; BAA29941; BAA29941.
DR   GeneID; 1443175; -.
DR   KEGG; pho:PH0847; -.
DR   eggNOG; arCOG04172; Archaea.
DR   OMA; YYGYGGF; -.
DR   OrthoDB; 65776at2157; -.
DR   Proteomes; UP000000752; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008675; F:2-dehydro-3-deoxy-phosphogluconate aldolase activity; IEA:UniProt.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   InterPro; IPR020625; Schiff_base-form_aldolases_AS.
DR   PANTHER; PTHR12128; PTHR12128; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   PRINTS; PR00146; DHPICSNTHASE.
DR   SMART; SM01130; DHDPS; 1.
DR   PROSITE; PS00666; DHDPS_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lyase; Schiff base.
FT   CHAIN           1..287
FT                   /note="Uncharacterized DapA-like lyase PH0847"
FT                   /id="PRO_0000103205"
FT   ACT_SITE        43
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        104
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        130
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        158
FT                   /note="Schiff-base intermediate with substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   287 AA;  31960 MW;  B33D7F2D33180223 CRC64;
     MEGVIVPLIT PFKEDHSIDF EALEWLIKFL EEKGVHGIFI NSTTGEFTSL SLEERKILAE
     KGREVTSRTY LVGTGSTSTF EVIELTKHAK EIGADGVVIV SPYYCRLKED AIFKHFSMVA
     EKVDIPIILY AIPSCANPIS LEVVRKLALE YSNVIGVKAS VDSLTYLSGL IEIKEERKDF
     KVFTGLDQYF LPNLILGGDG GIMACANFVP EIHLEVWNAF KKGNLEKAMN SARKLVKITK
     IYSIASSFAS AVKLAMVARG FPIKPILRPP YMMDGEEVFK KIKEIVS