ID   DAPAL_PYRIL             Reviewed;         301 AA.
AC   A1RRA5;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 2.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=Uncharacterized DapA-like lyase Pisl_0309;
DE            EC=4.-.-.-;
GN   Name=dapAL; OrderedLocusNames=Pisl_0309;
OS   Pyrobaculum islandicum (strain DSM 4184 / JCM 9189 / GEO3).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Pyrobaculum.
OX   NCBI_TaxID=384616;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 4184 / JCM 9189 / GEO3;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T.,
RA   Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Lowe T., Richardson P.;
RT   "Complete sequence of Pyrobaculum islandicum DSM 4184.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DapA family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABL87487.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; CP000504; ABL87487.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_053240262.1; NC_008701.1.
DR   AlphaFoldDB; A1RRA5; -.
DR   SMR; A1RRA5; -.
DR   STRING; 384616.Pisl_0309; -.
DR   EnsemblBacteria; ABL87487; ABL87487; Pisl_0309.
DR   GeneID; 4616637; -.
DR   KEGG; pis:Pisl_0309; -.
DR   eggNOG; arCOG04172; Archaea.
DR   HOGENOM; CLU_049343_5_1_2; -.
DR   OrthoDB; 65776at2157; -.
DR   Proteomes; UP000002595; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008675; F:2-dehydro-3-deoxy-phosphogluconate aldolase activity; IEA:UniProt.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   PANTHER; PTHR12128; PTHR12128; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   PRINTS; PR00146; DHPICSNTHASE.
DR   SMART; SM01130; DHDPS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lyase; Schiff base.
FT   CHAIN           1..301
FT                   /note="Uncharacterized DapA-like lyase Pisl_0309"
FT                   /id="PRO_0000340997"
FT   ACT_SITE        44
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        107
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        133
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        162
FT                   /note="Schiff-base intermediate with substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   301 AA;  33226 MW;  4199D275E1F7CD9A CRC64;
     MRIEGVIVAT VTPFTKDGVN YESLRTLLSK IVSEGYQGVF PTSSTGEVTK LTFEERVKVM
     EVAKEVAGGR ALVVAGTGTG DHLSTIEIAR RYKDVGVDAL LITPPYYIQY DWAAIYAFYK
     RVLDKVDMPT ILYTIPLATG YNIPVEVFEL VANEYSQVVG VKDSSGDFRY HLDLIHLLGK
     RLSVLQGLDL LFVPSLLMGA QGGVLAGPNF LGRITLEQYR LVKEGKIAEA VALHNKLMPL
     WRFMGGCGLV GKLGGKWPTL YKVATQIVRG IDMGPPREPL PPIDDKDRRE LEKLLKDLGL
     I