ID   DAPAL_PYRNV             Reviewed;         301 AA.
AC   B1YB29;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Uncharacterized DapA-like lyase Tneu_1812;
DE            EC=4.-.-.-;
GN   Name=dapAL; OrderedLocusNames=Tneu_1812;
OS   Pyrobaculum neutrophilum (strain DSM 2338 / JCM 9278 / NBRC 100436 /
OS   V24Sta) (Thermoproteus neutrophilus).
OC   Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae;
OC   Pyrobaculum.
OX   NCBI_TaxID=444157;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 2338 / JCM 9278 / NBRC 100436 / V24Sta;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Sims D., Brettin T., Detter J.C., Han C.,
RA   Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Biddle J.F., Zhang Z., Fitz-Gibbon S.T., Lowe T.M.,
RA   Saltikov C., House C.H., Richardson P.;
RT   "Complete sequence of Thermoproteus neutrophilus V24Sta.";
RL   Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the DapA family. {ECO:0000305}.
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DR   EMBL; CP001014; ACB40729.1; -; Genomic_DNA.
DR   AlphaFoldDB; B1YB29; -.
DR   SMR; B1YB29; -.
DR   STRING; 444157.Tneu_1812; -.
DR   EnsemblBacteria; ACB40729; ACB40729; Tneu_1812.
DR   KEGG; tne:Tneu_1812; -.
DR   eggNOG; arCOG04172; Archaea.
DR   HOGENOM; CLU_049343_5_1_2; -.
DR   OMA; LPPMRYK; -.
DR   Proteomes; UP000001694; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008675; F:2-dehydro-3-deoxy-phosphogluconate aldolase activity; IEA:UniProt.
DR   Gene3D; 3.20.20.70; -; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR002220; DapA-like.
DR   PANTHER; PTHR12128; PTHR12128; 1.
DR   Pfam; PF00701; DHDPS; 1.
DR   PIRSF; PIRSF001365; DHDPS; 1.
DR   PRINTS; PR00146; DHPICSNTHASE.
DR   SMART; SM01130; DHDPS; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Lyase; Schiff base.
FT   CHAIN           1..301
FT                   /note="Uncharacterized DapA-like lyase Tneu_1812"
FT                   /id="PRO_1000124073"
FT   ACT_SITE        44
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        107
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        133
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        162
FT                   /note="Schiff-base intermediate with substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   301 AA;  33177 MW;  8FE491F6C485BF98 CRC64;
     MRIEGVVVAT VTPFAKDGVN YEGLRALLSK IVEEGYQGVF PTSSTGEVTK LTFEERVKAM
     EVAKEVAGGR ALVVAGTGTG DHLSTIEIAR RYRDVGVDVL LITPPYYIQY DWAAVYAFYK
     RVLDKVDMPV VLYTIPLATG YNIPVEVFEL VANEYSQVVG VKDSSGDFRY HLDLIHLLGK
     RLSVLQGLDL LFVPSLLMGA HGGVLAGPNF LGRITLEQYR LVKEGKTAEA VALHNKLMPL
     WRFMGGCGLV GKLGGKWPTL YKVATQMVRG IDMGPPREPL PPIDDRDRKE LEKLLKDLGL
     I