ACT4_ARATH
ID ACT4_ARATH Reviewed; 377 AA.
AC P53494;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Actin-4;
GN Name=ACT4; OrderedLocusNames=At5g59370; ORFNames=F2O15.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC STRAIN=cv. Columbia;
RX PubMed=8771777; DOI=10.1046/j.1365-313x.1996.10020189.x;
RA Huang S., An Y.-Q., McDowell J.M., McKinney E.C., Meagher R.B.;
RT "The Arabidopsis thaliana ACT4/ACT12 actin gene subclass is strongly
RT expressed throughout pollen development.";
RL Plant J. 10:189-202(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY ORGANIZATION, AND CHARACTERIZATION.
RC STRAIN=cv. Columbia;
RX PubMed=8852856; DOI=10.1093/genetics/142.2.587;
RA McDowell J.M., Huang S., McKinney E.C., An Y.-Q., Meagher R.B.;
RT "Structure and evolution of the actin gene family in Arabidopsis
RT thaliana.";
RL Genetics 142:587-602(1996).
CC -!- FUNCTION: Actins are highly conserved proteins that are involved in
CC various types of cell motility and are ubiquitously expressed in all
CC eukaryotic cells. Essential component of cell cytoskeleton; plays an
CC important role in cytoplasmic streaming, cell shape determination, cell
CC division, organelle movement and extension growth. This is considered
CC as one of the reproductive actins.
CC -!- SUBUNIT: Polymerization of globular actin (G-actin) leads to a
CC structural filament (F-actin) in the form of a two-stranded helix. The
CC binding of profilin to monomeric G-actin cause the sequestration of
CC actin into profilactin complexes, and prevents the polymerization.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton.
CC -!- TISSUE SPECIFICITY: Little or no reproductive-gene expression is
CC detected in vegetative organs, such as root, stems, leaves, sepals and
CC petals.
CC -!- DEVELOPMENTAL STAGE: Expressed primarily in pollen.
CC -!- MISCELLANEOUS: There are 8 actin genes in A.thaliana.
CC -!- SIMILARITY: Belongs to the actin family. {ECO:0000305}.
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DR EMBL; U27980; AAB39403.1; -; Genomic_DNA.
DR EMBL; AB025604; BAA97473.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97176.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97177.1; -; Genomic_DNA.
DR EMBL; AY088111; AAM65657.1; -; mRNA.
DR PIR; S68108; S68108.
DR RefSeq; NP_001078769.1; NM_001085300.1.
DR RefSeq; NP_200745.1; NM_125328.4.
DR AlphaFoldDB; P53494; -.
DR SMR; P53494; -.
DR STRING; 3702.AT5G59370.2; -.
DR PaxDb; P53494; -.
DR PRIDE; P53494; -.
DR ProteomicsDB; 244794; -.
DR EnsemblPlants; AT5G59370.1; AT5G59370.1; AT5G59370.
DR EnsemblPlants; AT5G59370.2; AT5G59370.2; AT5G59370.
DR GeneID; 836056; -.
DR Gramene; AT5G59370.1; AT5G59370.1; AT5G59370.
DR Gramene; AT5G59370.2; AT5G59370.2; AT5G59370.
DR KEGG; ath:AT5G59370; -.
DR Araport; AT5G59370; -.
DR TAIR; locus:2148298; AT5G59370.
DR eggNOG; KOG0676; Eukaryota.
DR HOGENOM; CLU_027965_0_2_1; -.
DR InParanoid; P53494; -.
DR OMA; GRCCELK; -.
DR OrthoDB; 649708at2759; -.
DR PhylomeDB; P53494; -.
DR PRO; PR:P53494; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P53494; baseline and differential.
DR Genevisible; P53494; AT.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005200; F:structural constituent of cytoskeleton; ISS:TAIR.
DR InterPro; IPR004000; Actin.
DR InterPro; IPR020902; Actin/actin-like_CS.
DR InterPro; IPR004001; Actin_CS.
DR InterPro; IPR043129; ATPase_NBD.
DR PANTHER; PTHR11937; PTHR11937; 1.
DR Pfam; PF00022; Actin; 1.
DR PRINTS; PR00190; ACTIN.
DR SMART; SM00268; ACTIN; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00406; ACTINS_1; 1.
DR PROSITE; PS00432; ACTINS_2; 1.
DR PROSITE; PS01132; ACTINS_ACT_LIKE; 1.
PE 1: Evidence at protein level;
KW Acetylation; ATP-binding; Cytoplasm; Cytoskeleton; Nucleotide-binding;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q96292"
FT CHAIN 2..377
FT /note="Actin-4"
FT /id="PRO_0000088890"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q96292"
FT CONFLICT 154
FT /note="V -> F (in Ref. 4; AAM65657)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 377 AA; 41779 MW; 3BC5B633D2BB088A CRC64;
MADGEDIQPL VCDNGTGMVK AGFAGDDAPR AVFPSIVGRP RHTGVMVGMG QKDAYVGDEA
QSKRGILTLK YPIEHGIVNN WDDMEKIWHH TFYNELRVAP EEHPVLLTEA PLNPKANREK
MTQIMFETFN TPAMYVAIQA VLSLYASGRT TGIVLDSGDG VSHTVPIYEG YALPHAILRL
DLAGRDLTDH LMKILTERGY SFTTTAEREI VRDMKEKLSY IALDFEQELE TSKTSSSVEK
SFELPDGQVI TIGAERFRCP EVLFQPSMIG MENPGIHETT YNSIMKCDVD IRKDLYGNIV
LSGGTTMFGG IGDRMSKEIT ALAPSSMKIK VVAPPERKYS VWIGGSILAS LSTFQQMWIA
KAEYDESGPS IVHRKCF
