ID   DAPB_PHANO              Reviewed;         911 AA.
AC   Q0UVK7;
DT   27-JUL-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Probable dipeptidyl-aminopeptidase B;
DE            Short=DPAP B;
DE            EC=3.4.14.5;
GN   Name=DAPB; ORFNames=SNOG_04207;
OS   Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume
OS   blotch fungus) (Parastagonospora nodorum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC   Pleosporomycetidae; Pleosporales; Pleosporineae; Phaeosphaeriaceae;
OC   Parastagonospora.
OX   NCBI_TaxID=321614;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SN15 / ATCC MYA-4574 / FGSC 10173;
RX   PubMed=18024570; DOI=10.1105/tpc.107.052829;
RA   Hane J.K., Lowe R.G.T., Solomon P.S., Tan K.-C., Schoch C.L.,
RA   Spatafora J.W., Crous P.W., Kodira C.D., Birren B.W., Galagan J.E.,
RA   Torriani S.F.F., McDonald B.A., Oliver R.P.;
RT   "Dothideomycete-plant interactions illuminated by genome sequencing and EST
RT   analysis of the wheat pathogen Stagonospora nodorum.";
RL   Plant Cell 19:3347-3368(2007).
CC   -!- FUNCTION: Type IV dipeptidyl-peptidase which removes N-terminal
CC       dipeptides sequentially from polypeptides having unsubstituted N-
CC       termini provided that the penultimate residue is proline.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a
CC         polypeptide, preferentially when Yaa is Pro, provided Zaa is neither
CC         Pro nor hydroxyproline.; EC=3.4.14.5;
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Single-pass type
CC       II membrane protein {ECO:0000250}. Note=Lysosome-like vacuoles.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S9B family. {ECO:0000305}.
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DR   EMBL; CH445330; EAT87967.1; -; Genomic_DNA.
DR   RefSeq; XP_001794630.1; XM_001794578.1.
DR   AlphaFoldDB; Q0UVK7; -.
DR   SMR; Q0UVK7; -.
DR   STRING; 13684.SNOT_04207; -.
DR   ESTHER; phano-dapb; DPP4N_Peptidase_S9.
DR   MEROPS; S09.006; -.
DR   EnsemblFungi; SNOT_04207; SNOT_04207; SNOG_04207.
DR   GeneID; 5971498; -.
DR   KEGG; pno:SNOG_04207; -.
DR   eggNOG; KOG2100; Eukaryota.
DR   HOGENOM; CLU_006105_0_1_1; -.
DR   InParanoid; Q0UVK7; -.
DR   OMA; MRTPQEN; -.
DR   OrthoDB; 269253at2759; -.
DR   Proteomes; UP000001055; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0008239; F:dipeptidyl-peptidase activity; IBA:GO_Central.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR001375; Peptidase_S9.
DR   InterPro; IPR002469; Peptidase_S9B_N.
DR   Pfam; PF00930; DPPIV_N; 1.
DR   Pfam; PF00326; Peptidase_S9; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase; Glycoprotein; Hydrolase; Membrane; Protease;
KW   Reference proteome; Serine protease; Signal-anchor; Transmembrane;
KW   Transmembrane helix; Vacuole.
FT   CHAIN           1..911
FT                   /note="Probable dipeptidyl-aminopeptidase B"
FT                   /id="PRO_0000412158"
FT   TOPO_DOM        1..97
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        98..118
FT                   /note="Helical; Signal-anchor for type II membrane protein"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        119..911
FT                   /note="Vacuolar"
FT                   /evidence="ECO:0000255"
FT   REGION          1..39
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..17
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        18..39
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        755
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        832
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        865
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        268
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        564
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        809
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   911 AA;  103088 MW;  2B7D140A51D8324E CRC64;
     MPRPRAAKEE ETELLAQHQE SPRPSSDGSE ASASSISTTS LVLEHINDGG FNGARSKVSE
     KYTDDGNGDL GHARERFDIE DGKFHPLTPV DKKARRTLWI VGTICAVGWA LALVSFLMNG
     NYKHSSTRPH DPDASVTKGS GKKITLDNVL GGQFYPQSQS VSWIAGPKGE DGLLLEKGVS
     GKDYLVVEDI RSKGNTEAAG DKFTLMKKGN FQIGEDIFIY PSNVWPSADF KKVLVMSEQQ
     KNWRHSYTGL YWIFDVETQT GEPLDPENQS ARVQYASFSP QSDAVVFTRD NNLYLRKLDS
     QKVVKITHDG GSELFYGVPD WVYEEEVFQD NSATWWSEDG KYVAFLRTDE SMVPTYPVQY
     FVSRPSGKQP DAGKESYPEV REIKYPKAGA PNPIVTLQFY DIEKSEMFRV DIDNDFTDKD
     RLITEIVWAG KSGQVLVRET NRESDILKLI LIDVSKRTGK TIREENVAKL DGGWFEVSHK
     TTFIPADSSL GRANDGYIDS VIHEGYDHIG YFTPLDSDKP ILLTKGEWEV VEAPSRVDLK
     NNLVYYVSTE RGSMERHPYV VALNGTDKRE VMDHSGPAYY DSSFSTGGGY ALMSYQGPGI
     PWQKIVSTPS NTEKFEKVLE ENKALEKMVQ KHELPILKYQ TIDVDGFKLN VLERRPPHFS
     EKRKYPVLFY QYSGPGSQQV QRKFEVDFQS YIAANLGYIV VTVDGRGTGF LGRKLRCITR
     DNIGYYEAYD QIAAAKMWAA KKYVDAEKLA IWGWSYGGFT TLKTIEMDGG RTFKYGMAVA
     PVTDWRFYDS IYTERYMHTP QNNPTGYDNT SITDVHSLSQ NVRFLIMHGV ADDNVHMQNT
     LTLLDKLDVA GVENYDVHVF PDSDHSIYFH NAHKIVYDKL TWWLTNAFNG EWLKIQKVRP
     KAQADARSLG R