ID   3609L_ASFB7             Reviewed;         125 AA.
AC   Q65137;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Protein MGF 360-9L;
GN   OrderedLocusNames=BA71V-024; ORFNames=A125L;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   FUNCTION.
RX   PubMed=11238833; DOI=10.1128/jvi.75.7.3066-3076.2001;
RA   Zsak L., Lu Z., Burrage T.G., Neilan J.G., Kutish G.F., Moore D.M.,
RA   Rock D.L.;
RT   "African swine fever virus multigene family 360 and 530 genes are novel
RT   macrophage host range determinants.";
RL   J. Virol. 75:3066-3076(2001).
RN   [3]
RP   INDUCTION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
RN   [4]
RP   FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH HOST STAT1 AND STAT2, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=CN/GS/2018;
RX   PubMed=35076286; DOI=10.1128/mbio.02330-21;
RA   Zhang K., Yang B., Shen C., Zhang T., Hao Y., Zhang D., Liu H., Shi X.,
RA   Li G., Yang J., Li D., Zhu Z., Tian H., Yang F., Ru Y., Cao W.J., Guo J.,
RA   He J., Zheng H., Liu X.;
RT   "MGF360-9L Is a Major Virulence Factor Associated with the African Swine
RT   Fever Virus by Antagonizing the JAK/STAT Signaling Pathway.";
RL   MBio 0:0-0(2022).
CC   -!- FUNCTION: Plays a role in virus cell tropism, and may be required for
CC       efficient virus replication in macrophages (PubMed:11238833). In
CC       addition, inhibits IFN-beta-induced IFN-stimulated genes (ISGs)
CC       transcription. Mechanistically, degrades host STAT1 and STAT2 through
CC       apoptosis and ubiquitin-proteasome pathways respectively
CC       (PubMed:35076286). {ECO:0000269|PubMed:11238833,
CC       ECO:0000269|PubMed:35076286}.
CC   -!- SUBUNIT: Interacts with host STAT1; this interaction mediates STAT1
CC       degradation through apoptosis (PubMed:35076286). Interacts with host
CC       STAT2; this interaction mediates STAT2 degradation through the
CC       proteasome (PubMed:35076286). {ECO:0000269|PubMed:35076286}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000269|PubMed:35076286}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:32075923}.
CC   -!- DISRUPTION PHENOTYPE: Deletion causes mild pathological injury in pigs
CC       compared with WT. {ECO:0000269|PubMed:35076286}.
CC   -!- SIMILARITY: Belongs to the asfivirus MGF 360 family. {ECO:0000305}.
CC   -!- CAUTION: A error in sequencing might explain the fact that the this
CC       protein is shorter than any other ASFV MGF360-9L proteins.
CC       {ECO:0000305}.
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DR   EMBL; U18466; AAA65255.1; -; Genomic_DNA.
DR   RefSeq; NP_042719.1; NC_001659.2.
DR   GeneID; 22220407; -.
DR   KEGG; vg:22220407; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0039563; P:suppression by virus of host JAK-STAT cascade via inhibition of STAT1 activity; IEA:UniProtKB-KW.
DR   GO; GO:0039564; P:suppression by virus of host JAK-STAT cascade via inhibition of STAT2 activity; IEA:UniProtKB-KW.
DR   GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0042330; P:taxis; IEA:InterPro.
DR   InterPro; IPR002595; ASFV_MGF360.
DR   Pfam; PF01671; ASFV_360; 1.
PE   1: Evidence at protein level;
KW   Early protein; Host cytoplasm; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host interferon signaling pathway by virus;
KW   Inhibition of host STAT1 by virus; Inhibition of host STAT2 by virus;
KW   Reference proteome; Viral immunoevasion.
FT   CHAIN           1..125
FT                   /note="Protein MGF 360-9L"
FT                   /id="PRO_0000373267"
SQ   SEQUENCE   125 AA;  14501 MW;  711A3C9B7A1C3C3C CRC64;
     MDMDEMLRWA CRKNYNYLTI YYCCVALGAD INQAMFHSIQ FYNIGNIFFC IDLGANAFEE
     GKTLAHQKDN SFIASMLSLN CYSMNDSLSL KETDPEVIKR MLKDYHSKNL SIAHKHYIND
     GFNDI