ID   3609L_ASFM2             Reviewed;         353 AA.
AC   Q65121;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=Protein MGF 360-9L;
GN   OrderedLocusNames=Mal-028; ORFNames=LMW3CL;
OS   African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10500;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8030263; DOI=10.1006/viro.1994.1426;
RA   Yozawa T., Kutish G.F., Afonso C.L., Lu Z., Rock D.L.;
RT   "Two novel multigene families, 530 and 300, in the terminal variable
RT   regions of African swine fever virus genome.";
RL   Virology 202:997-1002(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role in virus cell tropism, and may be required for
CC       efficient virus replication in macrophages. In addition, inhibits IFN-
CC       beta-induced IFN-stimulated genes (ISGs) transcription.
CC       Mechanistically, degrades host STAT1 and STAT2 through apoptosis and
CC       ubiquitin-proteasome pathways respectively.
CC       {ECO:0000250|UniProtKB:Q65137}.
CC   -!- SUBUNIT: Interacts with host STAT1; this interaction mediates STAT1
CC       degradation through apoptosis. Interacts with host STAT2; this
CC       interaction mediates STAT2 degradation through the proteasome.
CC       {ECO:0000250|UniProtKB:Q65137}.
CC   -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000250|UniProtKB:Q65137}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the asfivirus MGF 360 family. {ECO:0000305}.
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DR   EMBL; U03762; AAA50534.1; -; Genomic_DNA.
DR   EMBL; AY261361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   SMR; Q65121; -.
DR   Proteomes; UP000000860; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0039563; P:suppression by virus of host JAK-STAT cascade via inhibition of STAT1 activity; IEA:UniProtKB-KW.
DR   GO; GO:0039564; P:suppression by virus of host JAK-STAT cascade via inhibition of STAT2 activity; IEA:UniProtKB-KW.
DR   GO; GO:0039502; P:suppression by virus of host type I interferon-mediated signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0042330; P:taxis; IEA:InterPro.
DR   InterPro; IPR002595; ASFV_MGF360.
DR   Pfam; PF01671; ASFV_360; 1.
PE   3: Inferred from homology;
KW   Early protein; Host cytoplasm; Host-virus interaction;
KW   Inhibition of host innate immune response by virus;
KW   Inhibition of host interferon signaling pathway by virus;
KW   Inhibition of host STAT1 by virus; Inhibition of host STAT2 by virus;
KW   Viral immunoevasion.
FT   CHAIN           1..353
FT                   /note="Protein MGF 360-9L"
FT                   /id="PRO_0000373271"
SQ   SEQUENCE   353 AA;  41636 MW;  613BBDD22197B6D3 CRC64;
     MVLSLQTLTK KVLARQYPAQ CHHHFLKCCG LWWHDGPIVY HQNQKKIWSS FFTDGVNINA
     ALVKAVKENN YDLIVLFTEW GANIDYSLLS VNTECTRDLC RELGAKEQLK QQEVLHFFNM
     VKRDLTGSNI ILCHEVFSHN PILETINRTK LRGIIYEQLE ALMENTDILS ELLTKYWYGM
     AVEFNLTKAI HYFYQRYAHL HQWRLMCALF YNNVFDLHEL YTKEKVRMDM DEMLKWACRK
     NYNYLTIYYC CIVLGADLNK AMFHSIQFYN LGNIFFCIDL GADAFEEGKT VAYQNDKSFI
     ASILSLNCYS KNDSLSLKET DPEVIKRMLK DYHSKNMSMA HKYYIKYGFD NID