DAPP1_MOUSE
ID DAPP1_MOUSE Reviewed; 280 AA.
AC Q9QXT1; Q9R178;
DT 05-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide;
DE Short=mDAPP1;
DE AltName: Full=B lymphocyte adapter protein Bam32;
DE AltName: Full=B-cell adapter molecule of 32 kDa;
GN Name=Dapp1; Synonyms=Bam32;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10432293; DOI=10.1042/bj3420007;
RA Dowler S., Currie R.A., Downes C.P., Alessi D.R.;
RT "DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides.";
RL Biochem. J. 342:7-12(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/cJ;
RX PubMed=10770799; DOI=10.1084/jem.191.8.1319;
RA Marshall A.J., Niiro H., Lerner C.G., Yun T.J., Thomas S., Disteche C.M.,
RA Clark E.A.;
RT "A novel B lymphocyte-associated adaptor protein, Bam32, regulates antigen
RT receptor signaling downstream of phosphatidylinositol 3-kinase.";
RL J. Exp. Med. 191:1319-1332(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-139 AND SER-141, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Mast cell;
RX PubMed=17947660; DOI=10.4049/jimmunol.179.9.5864;
RA Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y.,
RA Kawakami T., Salomon A.R.;
RT "Quantitative time-resolved phosphoproteomic analysis of mast cell
RT signaling.";
RL J. Immunol. 179:5864-5876(2007).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-139, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
CC -!- FUNCTION: May act as a B-cell-associated adapter that regulates B-cell
CC antigen receptor (BCR)-signaling downstream of PI3K.
CC -!- SUBUNIT: Interacts with PtdIns(3,4,5)P3 and PLCG2. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}. Note=Membrane-associated
CC after cell stimulation leading to its translocation. {ECO:0000250}.
CC -!- INDUCTION: Upon B-cell activation. {ECO:0000250}.
CC -!- PTM: Phosphorylated on tyrosine residues. {ECO:0000250}.
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DR EMBL; AF163255; AAD49698.1; -; mRNA.
DR EMBL; AF186023; AAF14579.1; -; mRNA.
DR EMBL; BC014759; AAH14759.1; -; mRNA.
DR CCDS; CCDS17864.1; -.
DR RefSeq; NP_036062.1; NM_011932.3.
DR AlphaFoldDB; Q9QXT1; -.
DR SMR; Q9QXT1; -.
DR STRING; 10090.ENSMUSP00000029806; -.
DR iPTMnet; Q9QXT1; -.
DR PhosphoSitePlus; Q9QXT1; -.
DR EPD; Q9QXT1; -.
DR MaxQB; Q9QXT1; -.
DR PaxDb; Q9QXT1; -.
DR PRIDE; Q9QXT1; -.
DR ProteomicsDB; 279827; -.
DR Antibodypedia; 25934; 445 antibodies from 37 providers.
DR DNASU; 26377; -.
DR Ensembl; ENSMUST00000029806; ENSMUSP00000029806; ENSMUSG00000028159.
DR GeneID; 26377; -.
DR KEGG; mmu:26377; -.
DR UCSC; uc008rmt.1; mouse.
DR CTD; 27071; -.
DR MGI; MGI:1347063; Dapp1.
DR VEuPathDB; HostDB:ENSMUSG00000028159; -.
DR eggNOG; KOG0017; Eukaryota.
DR GeneTree; ENSGT00910000144274; -.
DR HOGENOM; CLU_099070_0_0_1; -.
DR InParanoid; Q9QXT1; -.
DR OMA; ILRWKMS; -.
DR OrthoDB; 1061200at2759; -.
DR PhylomeDB; Q9QXT1; -.
DR TreeFam; TF105418; -.
DR Reactome; R-MMU-983695; Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
DR BioGRID-ORCS; 26377; 1 hit in 75 CRISPR screens.
DR PRO; PR:Q9QXT1; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q9QXT1; protein.
DR Bgee; ENSMUSG00000028159; Expressed in epithelium of small intestine and 159 other tissues.
DR ExpressionAtlas; Q9QXT1; baseline and differential.
DR Genevisible; Q9QXT1; MM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005547; F:phosphatidylinositol-3,4,5-trisphosphate binding; ISO:MGI.
DR GO; GO:0043325; F:phosphatidylinositol-3,4-bisphosphate binding; ISO:MGI.
DR CDD; cd10355; SH2_DAPP1_BAM32_like; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR Gene3D; 3.30.505.10; -; 1.
DR InterPro; IPR035843; DAPP1_SH2.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR000980; SH2.
DR InterPro; IPR036860; SH2_dom_sf.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00017; SH2; 1.
DR PRINTS; PR00401; SH2DOMAIN.
DR SMART; SM00233; PH; 1.
DR SMART; SM00252; SH2; 1.
DR SUPFAM; SSF55550; SSF55550; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50001; SH2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Membrane; Phosphoprotein; Reference proteome; SH2 domain.
FT CHAIN 1..280
FT /note="Dual adapter for phosphotyrosine and 3-
FT phosphotyrosine and 3-phosphoinositide"
FT /id="PRO_0000079786"
FT DOMAIN 35..129
FT /note="SH2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00191"
FT DOMAIN 164..259
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT MOD_RES 139
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:17947660,
FT ECO:0007744|PubMed:19144319"
FT MOD_RES 141
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17947660"
FT CONFLICT 90
FT /note="Y -> C (in Ref. 1; AAD49698)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 280 AA; 32167 MW; D15CF545A710F7F0 CRC64;
MGRAELLGGN MSTQDPSELW GRADGGTDLL QDLGWYHGNL TRHAAEALLL SNGRDGSYLL
RDSNEQTGLY SLSVRAKDSV KHFHVEYTGY SFKFGFNEYS SLKDFVKHFA NQPLIGSETG
TLMVLKHPYP REVEEPCIYE SVRVHTAMQT GRTENDLVPT APSLGTKEGY LTKQGGLVKT
WKTRWFTLQR NELKYFKDQM SPEPIRILDL TECSAVQFDY SQERVNCFCL VFPFRTFYLC
AKTGVEADEW IKILRWKLSK IRKQLDQGED TVRSRSFIFK
