DAP_CERS4
ID DAP_CERS4 Reviewed; 516 AA.
AC Q3IX78;
DT 03-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=D-aminopeptidase {ECO:0000255|HAMAP-Rule:MF_01960};
DE EC=3.4.11.19 {ECO:0000255|HAMAP-Rule:MF_01960};
GN Name=dap {ECO:0000255|HAMAP-Rule:MF_01960}; OrderedLocusNames=RHOS4_32880;
GN ORFNames=RSP_3246;
OS Cereibacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / CCUG
OS 31486 / LMG 2827 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Rhodobacter
OS sphaeroides).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
OC Rhodobacteraceae; Cereibacter.
OX NCBI_TaxID=272943;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 17023 / DSM 158 / JCM 6121 / CCUG 31486 / LMG 2827 / NBRC 12203
RC / NCIMB 8253 / ATH 2.4.1.;
RA Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA Hammon N., Israni S., Pitluck S., Richardson P., Mackenzie C.,
RA Choudhary M., Larimer F., Hauser L.J., Land M., Donohue T.J., Kaplan S.;
RT "Complete sequence of chromosome 2 of Rhodobacter sphaeroides 2.4.1.";
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Hydrolyzes N-terminal residues in D-amino acid-containing
CC peptides. {ECO:0000255|HAMAP-Rule:MF_01960}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Release of an N-terminal D-amino acid from a peptide, Xaa-|-
CC Yaa-, in which Xaa is preferably D-Ala, D-Ser or D-Thr. D-amino acid
CC amides and methyl esters also are hydrolyzed, as is glycine amide.;
CC EC=3.4.11.19; Evidence={ECO:0000255|HAMAP-Rule:MF_01960};
CC -!- ACTIVITY REGULATION: Inhibited by beta-lactam compounds such as 6-
CC aminopenicillic acid, 7-aminocephalosporanic acid, benzylpenicillin and
CC ampicillin. Inhibited by p-chloromercuribenzoate. {ECO:0000255|HAMAP-
CC Rule:MF_01960}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01960}.
CC -!- SIMILARITY: Belongs to the peptidase S12 family. {ECO:0000255|HAMAP-
CC Rule:MF_01960}.
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DR EMBL; CP000144; ABA80856.1; -; Genomic_DNA.
DR RefSeq; WP_011339153.1; NZ_CP030272.1.
DR RefSeq; YP_354757.1; NC_007494.2.
DR AlphaFoldDB; Q3IX78; -.
DR SMR; Q3IX78; -.
DR STRING; 272943.RSP_3246; -.
DR MEROPS; S12.002; -.
DR EnsemblBacteria; ABA80856; ABA80856; RSP_3246.
DR KEGG; rsp:RSP_3246; -.
DR PATRIC; fig|272943.9.peg.3676; -.
DR eggNOG; COG1680; Bacteria.
DR OMA; RDYWAMT; -.
DR PhylomeDB; Q3IX78; -.
DR Proteomes; UP000002703; Chromosome 2.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 2.40.128.50; -; 2.
DR Gene3D; 3.40.710.10; -; 1.
DR HAMAP; MF_01960; D_aminopeptidase; 1.
DR InterPro; IPR001466; Beta-lactam-related.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR027279; D_amino_pept/lipop_sf.
DR InterPro; IPR023645; DAP.
DR InterPro; IPR012856; DAP_B_dom.
DR Pfam; PF00144; Beta-lactamase; 1.
DR Pfam; PF07930; DAP_B; 1.
DR SUPFAM; SSF50886; SSF50886; 2.
DR SUPFAM; SSF56601; SSF56601; 1.
PE 3: Inferred from homology;
KW Aminopeptidase; Hydrolase; Protease; Reference proteome.
FT CHAIN 1..516
FT /note="D-aminopeptidase"
FT /id="PRO_0000250697"
FT REGION 476..486
FT /note="Important for specificity"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01960"
FT ACT_SITE 61
FT /note="Nucleophile"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01960"
FT ACT_SITE 64
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01960"
FT BINDING 480
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01960"
SQ SEQUENCE 516 AA; 55379 MW; BDA00A1D0FF7DD54 CRC64;
MTLDLDALDR ALDALPNLFR GPGGVAGVVK DGQVVASRAW GYADLTRRRP METGTRLPIC
SISKQFTCGA LLDTLGDTAA YDARVAEFLP QFEGPLPTLR QLCDNQSGLR DYWALTVLQG
AEAAQTFRRE DALPLIARMK TGHFPPGTAY SYCNCNFRIV SEILESETGR ALRDLYAERI
FGPAGMRTAE LTSDTRHPAD EVVGYEGSDA VGFFPADNGI FWIGDAGISA SLQDMLAYES
WIDATRNDEN SIYRRISVPP AYVCGTPASY GFGLSHETVA GVKVTGHGGA LRGFRAQRFH
AADERLSVVV IFNHEASAHA AASSLLAAAL GHEAPKGARP EGWAGQWLDP ESGLLLRVGE
DAEGLTLRFA TGPDRLTAGE DGVPRGAGVS LAREGAMLVM NRTSDNLTVR AEPLPVVAVA
DAGEIAGRYH ARELEADLVI EARDGGAYAG FEGLLGAGPM ERLHPVGPDV WIVTTRRSMD
APAPGDWTLQ VRREGGAVTG LRLGCWLARR IDYARV
