DARA_BACSU
ID DARA_BACSU Reviewed; 109 AA.
AC P37538;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Cyclic di-AMP receptor A {ECO:0000303|PubMed:25433025};
DE Short=c-di-AMP receptor A {ECO:0000303|PubMed:25433025};
DE AltName: Full=PII-like signal transduction protein DarA {ECO:0000303|PubMed:25433025};
GN Name=darA {ECO:0000303|PubMed:25433025}; Synonyms=yaaQ;
GN OrderedLocusNames=BSU00290;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=7584024; DOI=10.1093/dnares/1.1.1;
RA Ogasawara N., Nakai S., Yoshikawa H.;
RT "Systematic sequencing of the 180 kilobase region of the Bacillus subtilis
RT chromosome containing the replication origin.";
RL DNA Res. 1:1-14(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3] {ECO:0007744|PDB:4RLE}
RP X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) IN COMPLEX WITH C-DI-AMP, FUNCTION,
RP SUBUNIT, DOMAIN, AND DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=25433025; DOI=10.1074/jbc.m114.619619;
RA Gundlach J., Dickmanns A., Schroder-Tittmann K., Neumann P., Kaesler J.,
RA Kampf J., Herzberg C., Hammer E., Schwede F., Kaever V., Tittmann K.,
RA Stulke J., Ficner R.;
RT "Identification, characterization, and structure analysis of the cyclic di-
RT AMP-binding PII-like signal transduction protein DarA.";
RL J. Biol. Chem. 290:3069-3080(2015).
CC -!- FUNCTION: Binds cyclic di-AMP (c-di-AMP) and is probably involved in c-
CC di-AMP-mediated signaling pathways. In vitro, can also bind cyclic GMP-
CC AMP (3'3'-cGAMP), with lower affinity, but not c-di-GMP or 2'3'-cGAMP.
CC {ECO:0000269|PubMed:25433025}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:25433025}.
CC -!- DOMAIN: Binds three molecules of c-di-AMP, each in a pocket located
CC between two subunits. c-di-AMP binding is accompanied by conformational
CC changes of both the fold and the position of the B-loop.
CC {ECO:0000269|PubMed:25433025}.
CC -!- DISRUPTION PHENOTYPE: Not essential. {ECO:0000269|PubMed:25433025}.
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DR EMBL; D26185; BAA05265.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB11805.1; -; Genomic_DNA.
DR PIR; S66059; S66059.
DR RefSeq; NP_387910.1; NC_000964.3.
DR RefSeq; WP_003242755.1; NZ_JNCM01000028.1.
DR PDB; 4RLE; X-ray; 1.30 A; A=1-109.
DR PDBsum; 4RLE; -.
DR AlphaFoldDB; P37538; -.
DR SMR; P37538; -.
DR STRING; 224308.BSU00290; -.
DR jPOST; P37538; -.
DR PaxDb; P37538; -.
DR PRIDE; P37538; -.
DR EnsemblBacteria; CAB11805; CAB11805; BSU_00290.
DR GeneID; 938610; -.
DR KEGG; bsu:BSU00290; -.
DR PATRIC; fig|224308.179.peg.29; -.
DR eggNOG; COG3870; Bacteria.
DR InParanoid; P37538; -.
DR OMA; TFMIGIE; -.
DR PhylomeDB; P37538; -.
DR BioCyc; BSUB:BSU00290-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR Gene3D; 3.30.70.120; -; 1.
DR InterPro; IPR010375; CdAMP_rec.
DR InterPro; IPR011322; N-reg_PII-like_a/b.
DR InterPro; IPR015867; N-reg_PII/ATP_PRibTrfase_C.
DR PANTHER; PTHR38456; PTHR38456; 1.
DR Pfam; PF06153; CdAMP_rec; 1.
DR SUPFAM; SSF54913; SSF54913; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome.
FT CHAIN 1..109
FT /note="Cyclic di-AMP receptor A"
FT /id="PRO_0000049435"
FT BINDING 21
FT /ligand="3',3'-c-di-AMP"
FT /ligand_id="ChEBI:CHEBI:71500"
FT /evidence="ECO:0000269|PubMed:25433025"
FT BINDING 25..28
FT /ligand="3',3'-c-di-AMP"
FT /ligand_id="ChEBI:CHEBI:71500"
FT /evidence="ECO:0000269|PubMed:25433025"
FT BINDING 35..37
FT /ligand="3',3'-c-di-AMP"
FT /ligand_id="ChEBI:CHEBI:71500"
FT /evidence="ECO:0000269|PubMed:25433025"
FT BINDING 41
FT /ligand="3',3'-c-di-AMP"
FT /ligand_id="ChEBI:CHEBI:71500"
FT /evidence="ECO:0000269|PubMed:25433025"
FT BINDING 47
FT /ligand="3',3'-c-di-AMP"
FT /ligand_id="ChEBI:CHEBI:71500"
FT /evidence="ECO:0000269|PubMed:25433025"
FT BINDING 92..94
FT /ligand="3',3'-c-di-AMP"
FT /ligand_id="ChEBI:CHEBI:71500"
FT /evidence="ECO:0000269|PubMed:25433025"
FT STRAND 1..8
FT /evidence="ECO:0007829|PDB:4RLE"
FT HELIX 10..22
FT /evidence="ECO:0007829|PDB:4RLE"
FT STRAND 28..33
FT /evidence="ECO:0007829|PDB:4RLE"
FT TURN 35..37
FT /evidence="ECO:0007829|PDB:4RLE"
FT STRAND 39..49
FT /evidence="ECO:0007829|PDB:4RLE"
FT HELIX 50..52
FT /evidence="ECO:0007829|PDB:4RLE"
FT HELIX 53..63
FT /evidence="ECO:0007829|PDB:4RLE"
FT STRAND 67..70
FT /evidence="ECO:0007829|PDB:4RLE"
FT TURN 76..83
FT /evidence="ECO:0007829|PDB:4RLE"
FT STRAND 90..93
FT /evidence="ECO:0007829|PDB:4RLE"
FT STRAND 95..102
FT /evidence="ECO:0007829|PDB:4RLE"
SQ SEQUENCE 109 AA; 11967 MW; 15827F78355C0981 CRC64;
MKLIVAVVQD QDSNRLLKTL TDHNFRVTKL ATTGGFLKSG NTTFMIGVED IRVNKALSLI
KENGQKRDQM IAPVSPMGGN ADSYVPYPVE VEVGGATVFV LPVDEFHQF
