DARA_DICDI
ID DARA_DICDI Reviewed; 766 AA.
AC O76187; Q54IE6;
DT 20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Darlin;
DE AltName: Full=Armadillo-like protein A;
GN Name=darA; ORFNames=DDB_G0288771;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20-33; 62-79 AND
RP 235-256, FUNCTION, INTERACTION WITH RACE AND RACC, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=9802899; DOI=10.1091/mbc.9.11.3095;
RA Vithalani K.K., Parent C.A., Thorn E.M., Penn M., Larochelle D.A.,
RA Devreotes P.N., De Lozanne A.;
RT "Identification of darlin, a Dictyostelium protein with Armadillo-like
RT repeats that binds to small GTPases and is important for the proper
RT aggregation of developing cells.";
RL Mol. Biol. Cell 9:3095-3106(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Part of a signaling pathway that initiates the aggregation
CC and leads to the formation of aggregation centers or streams. Not
CC essential for cytokinesis, pinocytosis or phagocytosis. Not essential
CC for development, except in starvation-induced aggregation.
CC {ECO:0000269|PubMed:9802899}.
CC -!- SUBUNIT: Binds to small GTPases racE, racC but not rab21. Binds
CC preferentially to GDP-bound racE.
CC -!- INTERACTION:
CC O76187; Q23862: racE; NbExp=2; IntAct=EBI-2889579, EBI-2118748;
CC -!- DISRUPTION PHENOTYPE: Not able to initiate development when placed in
CC starvation buffer. Defect in ability of cells to aggregate in response
CC to starvation. They were unable to form aggregation centers and
CC streams, possibly because of a defect in cAMP relay signaling.
CC {ECO:0000269|PubMed:9802899}.
CC -!- SIMILARITY: Belongs to the RAP1GDS1 family. {ECO:0000305}.
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DR EMBL; AF006055; AAC34253.1; -; Genomic_DNA.
DR EMBL; AAFI02000125; EAL63007.1; -; Genomic_DNA.
DR PIR; T03218; T03218.
DR RefSeq; XP_636531.1; XM_631439.1.
DR AlphaFoldDB; O76187; -.
DR IntAct; O76187; 4.
DR STRING; 44689.DDB0191300; -.
DR PaxDb; O76187; -.
DR EnsemblProtists; EAL63007; EAL63007; DDB_G0288771.
DR GeneID; 8626817; -.
DR KEGG; ddi:DDB_G0288771; -.
DR dictyBase; DDB_G0288771; darA.
DR eggNOG; ENOG502REAH; Eukaryota.
DR HOGENOM; CLU_364653_0_0_1; -.
DR InParanoid; O76187; -.
DR OMA; FIQLEFF; -.
DR PhylomeDB; O76187; -.
DR PRO; PR:O76187; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0031267; F:small GTPase binding; IPI:UniProtKB.
DR GO; GO:0031152; P:aggregation involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR040144; RAP1GDS1.
DR PANTHER; PTHR10957; PTHR10957; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Reference proteome; Repeat.
FT CHAIN 1..766
FT /note="Darlin"
FT /id="PRO_0000392078"
FT REPEAT 82..119
FT /note="ARM 1"
FT REPEAT 167..208
FT /note="ARM 2"
FT REPEAT 423..464
FT /note="ARM 3"
FT REPEAT 465..537
FT /note="ARM 4"
FT REPEAT 610..649
FT /note="ARM 5"
FT REGION 561..585
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 766 AA; 87693 MW; 70256FF12B213EC9 CRC64;
MEEIQKLINE LGGSQYKDKD TEHYSEEAVE LLIKKIDNKS KGKTVIESNT LIYKTLADWS
KVEDNRETII RSPSNVIEKT FQLFEFLLPN GVETLNTDTT VEQSELFSMV CRLLGNLTYE
NAPNREFIFD KTPSILKYIS SFVSQTKYQS LQRTSCAAIA NLSSETDFIQ LEFFKLGVVS
ILIDLICREG TTDEVNQMAI KAFNNLVDNE NTQVDIHFKE IHRLLGQLKK SLNKEGYYEN
SFANDLVSSL STLSLNKDLQ KEILKEGFLN DLIELIEESN VHREMINQFE DDDIEKDKDI
SIAPSTSELI FKLADNDDYR QYFYNKERDG NDANNILDRM IKIMTSPPPA YKDGDSKAQL
KALDVAKVKK NITKTIALCS LEDDVIDKFI KTPKIFVDLL VDTEDTERIV NGEMIIGNLA
RSEPNCRLLN SYNVIELIAD IMKKFPNFQP IQHLGLSSIR NLTLPTINKG FKPSSTLMED
VVFNSKVHNQ VIQFAALSLI KNFIGCDQSN LKLFLEFPNA LQSLLDLANG RVPASMDDES
DQQEQLQLDD AKIQEIEEKF EEKEKTIEKT DEKTDEKTNE KKQSKKDMRV IYEATRLLLR
FLDNSELSNH QEKMKQLIEE SVEPFFSLLQ SPFPILQVEG AKGLVLLIKH DKQLFLSRPS
WVKDLVEVLS LSIIPFQQLS REQSTITTDP NHQKLIAQIQ SNCNFSTELQ NVILSNIFYN
FSLDESICKK MKDQNVISEL KTLKSKQDAP QSLTNLIQKI LVQLTI
