ID   ACTO_ACACA              Reviewed;          88 AA.
AC   P18281;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   25-MAY-2022, entry version 51.
DE   RecName: Full=Actobindin;
OS   Acanthamoeba castellanii (Amoeba).
OC   Eukaryota; Amoebozoa; Discosea; Longamoebia; Centramoebida;
OC   Acanthamoebidae; Acanthamoeba.
OX   NCBI_TaxID=5755;
RN   [1]
RP   PROTEIN SEQUENCE, ACETYLATION AT MET-1, AND METHYLATION AT LYS-35 AND
RP   LYS-72.
RX   PubMed=2376577; DOI=10.1016/s0021-9258(19)38230-4;
RA   Vandekerckhove J., van Damme J., Vancompernolle K., Bubb M.R.,
RA   Lambooy P.K., Korn E.D.;
RT   "The covalent structure of Acanthamoeba actobindin.";
RL   J. Biol. Chem. 265:12801-12805(1990).
CC   -!- FUNCTION: Is able to bind two actin monomers at high concentrations of
CC       G-actin.
CC   -!- SUBUNIT: Monomer.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; A36614; A36614.
DR   AlphaFoldDB; P18281; -.
DR   ELM; P18281; -.
DR   iPTMnet; P18281; -.
DR   VEuPathDB; AmoebaDB:ACA1_256510; -.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   InterPro; IPR016365; Actobindin.
DR   InterPro; IPR003124; WH2_dom.
DR   Pfam; PF02205; WH2; 1.
DR   PIRSF; PIRSF002724; Actobindin; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Actin-binding; Direct protein sequencing; Methylation.
FT   CHAIN           1..88
FT                   /note="Actobindin"
FT                   /id="PRO_0000064444"
FT   DOMAIN          37..54
FT                   /note="WH2"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         1
FT                   /note="N-acetylmethionine"
FT                   /evidence="ECO:0000269|PubMed:2376577"
FT   MOD_RES         35
FT                   /note="N6,N6,N6-trimethyllysine"
FT                   /evidence="ECO:0000269|PubMed:2376577"
FT   MOD_RES         72
FT                   /note="N6,N6,N6-trimethyllysine"
FT                   /evidence="ECO:0000269|PubMed:2376577"
SQ   SEQUENCE   88 AA;  9554 MW;  E814A5C521603DA6 CRC64;
     MNPELQSAIG QGAALKHAET VDKSAPQIEN VTVKKVDRSS FLEEVAKPHE LKHAETVDKS
     GPAIPEDVHV KKVDRGAFLS EIEKAAKQ