ID   DAS2_YEAST              Reviewed;         232 AA.
AC   Q12084; D6VS06;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 171.
DE   RecName: Full=Putative uridine kinase DAS2;
DE            EC=2.7.1.48;
DE   AltName: Full=DST1-delta 6-azauracil sensitivity protein 2;
DE   AltName: Full=Regulator of rDNA transcription 3;
GN   Name=DAS2; Synonyms=RRT3; OrderedLocusNames=YDR020C; ORFNames=PZE232;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8896275;
RX   DOI=10.1002/(sici)1097-0061(199609)12:10b<1085::aid-yea9>3.3.co;2-s;
RA   Eide L.G., Sander C., Prydz H.;
RT   "Sequencing and analysis of a 35.4 kb region on the right arm of chromosome
RT   IV from Saccharomyces cerevisiae reveal 23 open reading frames.";
RL   Yeast 12:1085-1090(1996).
RN   [2]
RP   ERRATUM OF PUBMED:8896275.
RA   Eide L.G., Sander C., Prydz H.;
RL   Yeast 13:189-189(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   FUNCTION.
RX   PubMed=19270272; DOI=10.1534/genetics.108.100313;
RA   Hontz R.D., Niederer R.O., Johnson J.M., Smith J.S.;
RT   "Genetic identification of factors that modulate ribosomal DNA
RT   transcription in Saccharomyces cerevisiae.";
RL   Genetics 182:105-119(2009).
CC   -!- FUNCTION: Putative uridine kinase identified in a screen for mutants
CC       with increased levels of rDNA transcription.
CC       {ECO:0000269|PubMed:19270272}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + uridine = ADP + H(+) + UMP; Xref=Rhea:RHEA:16825,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16704, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57865, ChEBI:CHEBI:456216; EC=2.7.1.48;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + cytidine = ADP + CMP + H(+); Xref=Rhea:RHEA:24674,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:17562, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:60377, ChEBI:CHEBI:456216; EC=2.7.1.48;
CC   -!- PATHWAY: Pyrimidine metabolism; CTP biosynthesis via salvage pathway;
CC       CTP from cytidine: step 1/3.
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via salvage pathway;
CC       UMP from uridine: step 1/1.
CC   -!- INTERACTION:
CC       Q12084; P27515: URK1; NbExp=4; IntAct=EBI-35824, EBI-20151;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}. Nucleus
CC       {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 4280 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the uridine kinase family. {ECO:0000305}.
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DR   EMBL; X95966; CAA65212.1; -; Genomic_DNA.
DR   EMBL; Z49770; CAA89845.1; -; Genomic_DNA.
DR   EMBL; Z74316; CAA98841.1; -; Genomic_DNA.
DR   EMBL; AY558571; AAS56897.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA11866.1; -; Genomic_DNA.
DR   PIR; S54643; S54643.
DR   RefSeq; NP_010303.3; NM_001180328.3.
DR   AlphaFoldDB; Q12084; -.
DR   SMR; Q12084; -.
DR   BioGRID; 32070; 81.
DR   DIP; DIP-1721N; -.
DR   IntAct; Q12084; 1.
DR   MINT; Q12084; -.
DR   STRING; 4932.YDR020C; -.
DR   iPTMnet; Q12084; -.
DR   MaxQB; Q12084; -.
DR   PaxDb; Q12084; -.
DR   PRIDE; Q12084; -.
DR   EnsemblFungi; YDR020C_mRNA; YDR020C; YDR020C.
DR   GeneID; 851583; -.
DR   KEGG; sce:YDR020C; -.
DR   SGD; S000002427; DAS2.
DR   VEuPathDB; FungiDB:YDR020C; -.
DR   eggNOG; KOG4203; Eukaryota.
DR   GeneTree; ENSGT01020000230412; -.
DR   HOGENOM; CLU_021278_1_0_1; -.
DR   InParanoid; Q12084; -.
DR   OMA; HLDFQAE; -.
DR   BioCyc; YEAST:G3O-29637-MON; -.
DR   Reactome; R-SCE-73614; Pyrimidine salvage.
DR   UniPathway; UPA00574; UER00637.
DR   UniPathway; UPA00579; UER00640.
DR   PRO; PR:Q12084; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; Q12084; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004849; F:uridine kinase activity; IBA:GO_Central.
DR   GO; GO:0044211; P:CTP salvage; IEA:UniProtKB-UniPathway.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0008655; P:pyrimidine-containing compound salvage; IBA:GO_Central.
DR   GO; GO:0044206; P:UMP salvage; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR006083; PRK/URK.
DR   Pfam; PF00485; PRK; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Cytoplasm; Kinase; Nucleotide-binding; Nucleus;
KW   Reference proteome; Transferase.
FT   CHAIN           1..232
FT                   /note="Putative uridine kinase DAS2"
FT                   /id="PRO_0000242489"
FT   BINDING         17..24
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   232 AA;  26595 MW;  4171B063EE750613 CRC64;
     MDRKAVEEKR IVISIGGGHA TGVGAIALDL QNTFKSLYNS INIRVINLDN MIEGNIKSYN
     NNDYDFDNIL NLVYEKHAVT SQNDMIQHDY EDPIDLIIVC GCYALYDKRI NEISQLKVFL
     DSDADKRLIS LIKKKNVGSN EQLAQLITEY MDHLRPEMQQ YIEPTRTFAD LIIPSTNENL
     GRAVLVDGIV KAIEDTKSQI EGNNTNNKIR PRLWDFEAET MDLEKDRYYD LS