DASB_STRCO
ID DASB_STRCO Reviewed; 328 AA.
AC Q9K490;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Diacetylchitobiose uptake system permease protein DasB {ECO:0000305};
GN Name=dasB {ECO:0000303|PubMed:17351098};
GN OrderedLocusNames=SCO5233 {ECO:0000312|EMBL:CAB94618.1};
OS Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces; Streptomyces albidoflavus group.
OX NCBI_TaxID=100226;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=12000953; DOI=10.1038/417141a;
RA Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT A3(2).";
RL Nature 417:141-147(2002).
RN [2]
RP FUNCTION, AND INDUCTION.
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=17351098; DOI=10.1128/aem.02612-06;
RA Saito A., Shinya T., Miyamoto K., Yokoyama T., Kaku H., Minami E.,
RA Shibuya N., Tsujibo H., Nagata Y., Ando A., Fujii T., Miyashita K.;
RT "The dasABC gene cluster, adjacent to dasR, encodes a novel ABC transporter
RT for the uptake of N,N'-diacetylchitobiose in Streptomyces coelicolor
RT A3(2).";
RL Appl. Environ. Microbiol. 73:3000-3008(2007).
RN [3]
RP SUBUNIT.
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=18957589; DOI=10.1099/mic.0.2008/019612-0;
RA Saito A., Fujii T., Shinya T., Shibuya N., Ando A., Miyashita K.;
RT "The msiK gene, encoding the ATP-hydrolysing component of N,N'-
RT diacetylchitobiose ABC transporters, is essential for induction of
RT chitinase production in Streptomyces coelicolor A3(2).";
RL Microbiology 154:3358-3365(2008).
CC -!- FUNCTION: Part of the ABC transporter complex DasABC-MsiK involved in
CC N,N'-diacetylchitobiose ((GlcNAc)2) uptake. Responsible for the
CC translocation of the substrate across the membrane.
CC {ECO:0000305|PubMed:17351098}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (MsiK),
CC two transmembrane proteins (DasB and DasC) and a solute-binding protein
CC (DasA). {ECO:0000305|PubMed:18957589}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- INDUCTION: Induced by (GlcNAc)2 and (GlcNAc)3.
CC {ECO:0000269|PubMed:17351098}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. {ECO:0000305}.
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DR EMBL; AL939123; CAB94618.1; -; Genomic_DNA.
DR RefSeq; NP_629380.1; NC_003888.3.
DR RefSeq; WP_003973738.1; NZ_VNID01000008.1.
DR AlphaFoldDB; Q9K490; -.
DR SMR; Q9K490; -.
DR STRING; 100226.SCO5233; -.
DR GeneID; 1100674; -.
DR KEGG; sco:SCO5233; -.
DR PATRIC; fig|100226.15.peg.5316; -.
DR eggNOG; COG1175; Bacteria.
DR HOGENOM; CLU_016047_0_3_11; -.
DR InParanoid; Q9K490; -.
DR OMA; LGWHSMA; -.
DR PhylomeDB; Q9K490; -.
DR Proteomes; UP000001973; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008643; P:carbohydrate transport; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IEA:InterPro.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Sugar transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..328
FT /note="Diacetylchitobiose uptake system permease protein
FT DasB"
FT /id="PRO_0000447874"
FT TRANSMEM 36..56
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 100..316
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 328 AA; 36132 MW; 1E117FD3992483D7 CRC64;
MTVQTERPPS GPSDVRKADG GGTGGTRARA ASRAGALAPY LLLLPAAAAT VLLLGWPLVK
DGLLSFQNLN MAQLIQHVTE WTGFDNYKEV LTGEDFWRVT VRSIIFTAVN VVLTMVVGGL
IGLLLARLGR VMRFVLMIGL VLAWAMPVVA ATTVYQWLFA QRFGVVNWVL DKLGWHSMAD
FSWTGSQFST FFVVTVLIVW MSVPFVAINL YAATTTIPDE LYEAAALDGA GMWRSFTSVT
LPFLRPFLYA TTFLEVIWIF KAFVQVYTFN GGGPDRLTEI LPVYAYIEGV GNQHYGMGAA
IAVLTILILL GLTAYYLRIV LKQEEDEL
